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A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae

An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular aging in the budding yeast Saccharomyces cerevisiae; however, the underlying mechanism remains unknown. Preferential accumulation of Pma1p, which pumps cytoplasmic protons out of cells, at the plasma mem...

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Autores principales: Yoon, So-Young, Jang, Eunhong, Ko, Naho, Kim, Minseok, Kim, Su Yoon, Moon, Yeojin, Nam, Jeong-Seok, Lee, Sunjae, Jun, Youngsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874555/
https://www.ncbi.nlm.nih.gov/pubmed/35216480
http://dx.doi.org/10.3390/ijms23042364
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author Yoon, So-Young
Jang, Eunhong
Ko, Naho
Kim, Minseok
Kim, Su Yoon
Moon, Yeojin
Nam, Jeong-Seok
Lee, Sunjae
Jun, Youngsoo
author_facet Yoon, So-Young
Jang, Eunhong
Ko, Naho
Kim, Minseok
Kim, Su Yoon
Moon, Yeojin
Nam, Jeong-Seok
Lee, Sunjae
Jun, Youngsoo
author_sort Yoon, So-Young
collection PubMed
description An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular aging in the budding yeast Saccharomyces cerevisiae; however, the underlying mechanism remains unknown. Preferential accumulation of Pma1p, which pumps cytoplasmic protons out of cells, at the plasma membrane of mother cells, but not of their newly-formed daughter cells, is believed to be responsible for the pH increase in mother cells by reducing the level of cytoplasmic protons. This, in turn, decreases the acidity of vacuoles, which is well correlated with aging of yeast cells. In this study, to identify genes that regulate the preferential accumulation of Pma1p in mother cells, we performed a genome-wide screen using a collection of single gene deletion yeast strains. A subset of genes involved in the endocytic pathway, such as VPS8, VPS9, and VPS21, was important for Pma1p accumulation. Unexpectedly, however, there was little correlation between deletion of each of these genes and the replicative lifespan of yeast, suggesting that Pma1p accumulation in mother cells is not the key determinant that underlies aging of mother cells.
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spelling pubmed-88745552022-02-26 A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae Yoon, So-Young Jang, Eunhong Ko, Naho Kim, Minseok Kim, Su Yoon Moon, Yeojin Nam, Jeong-Seok Lee, Sunjae Jun, Youngsoo Int J Mol Sci Article An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular aging in the budding yeast Saccharomyces cerevisiae; however, the underlying mechanism remains unknown. Preferential accumulation of Pma1p, which pumps cytoplasmic protons out of cells, at the plasma membrane of mother cells, but not of their newly-formed daughter cells, is believed to be responsible for the pH increase in mother cells by reducing the level of cytoplasmic protons. This, in turn, decreases the acidity of vacuoles, which is well correlated with aging of yeast cells. In this study, to identify genes that regulate the preferential accumulation of Pma1p in mother cells, we performed a genome-wide screen using a collection of single gene deletion yeast strains. A subset of genes involved in the endocytic pathway, such as VPS8, VPS9, and VPS21, was important for Pma1p accumulation. Unexpectedly, however, there was little correlation between deletion of each of these genes and the replicative lifespan of yeast, suggesting that Pma1p accumulation in mother cells is not the key determinant that underlies aging of mother cells. MDPI 2022-02-21 /pmc/articles/PMC8874555/ /pubmed/35216480 http://dx.doi.org/10.3390/ijms23042364 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoon, So-Young
Jang, Eunhong
Ko, Naho
Kim, Minseok
Kim, Su Yoon
Moon, Yeojin
Nam, Jeong-Seok
Lee, Sunjae
Jun, Youngsoo
A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title_full A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title_fullStr A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title_full_unstemmed A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title_short A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
title_sort genome-wide screen reveals that endocytic genes are important for pma1p asymmetry during cell division in saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874555/
https://www.ncbi.nlm.nih.gov/pubmed/35216480
http://dx.doi.org/10.3390/ijms23042364
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