Cargando…

The Removal of Time–Concentration Data Points from Progress Curves Improves the Determination of K(m): The Example of Paraoxonase 1

Several approaches for determining an enzyme’s kinetic parameter K(m) (Michaelis constant) from progress curves have been developed in recent decades. In the present article, we compare different approaches on a set of experimental measurements of lactonase activity of paraoxonase 1 (PON1): (1) a di...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrič, Boštjan, Goličnik, Marko, Bavec, Aljoša
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874660/
https://www.ncbi.nlm.nih.gov/pubmed/35209091
http://dx.doi.org/10.3390/molecules27041306