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The Removal of Time–Concentration Data Points from Progress Curves Improves the Determination of K(m): The Example of Paraoxonase 1
Several approaches for determining an enzyme’s kinetic parameter K(m) (Michaelis constant) from progress curves have been developed in recent decades. In the present article, we compare different approaches on a set of experimental measurements of lactonase activity of paraoxonase 1 (PON1): (1) a di...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874660/ https://www.ncbi.nlm.nih.gov/pubmed/35209091 http://dx.doi.org/10.3390/molecules27041306 |