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Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection
Point-of-care (POC) tests capable of individual health monitoring, transmission reduction, and contact tracing are especially important in a pandemic such as the coronavirus disease 2019 (COVID-19). We develop a disposable POC cartridge that can be mass produced to detect the SARS-CoV-2 N gene throu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874717/ https://www.ncbi.nlm.nih.gov/pubmed/35208320 http://dx.doi.org/10.3390/mi13020196 |
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author | Ho, Kuan-Lun Liao, Hong-Yu Liu, Helene Minyi Lu, Yen-Wen Yeh, Pin-Kuan Chang, Justin Yu Fan, Shih-Kang |
author_facet | Ho, Kuan-Lun Liao, Hong-Yu Liu, Helene Minyi Lu, Yen-Wen Yeh, Pin-Kuan Chang, Justin Yu Fan, Shih-Kang |
author_sort | Ho, Kuan-Lun |
collection | PubMed |
description | Point-of-care (POC) tests capable of individual health monitoring, transmission reduction, and contact tracing are especially important in a pandemic such as the coronavirus disease 2019 (COVID-19). We develop a disposable POC cartridge that can be mass produced to detect the SARS-CoV-2 N gene through real-time quantitative polymerase chain reaction (qPCR) based on digital microfluidics (DMF). Several critical parameters are studied and improved, including droplet volume consistency, temperature uniformity, and fluorescence intensity linearity on the designed DMF cartridge. The qPCR results showed high accuracy and efficiency for two primer-probe sets of N1 and N2 target regions of the SARS-CoV-2 N gene on the DMF cartridge. Having multiple droplet tracks for qPCR, the presented DMF cartridge can perform multiple tests and controls at once. |
format | Online Article Text |
id | pubmed-8874717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88747172022-02-26 Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection Ho, Kuan-Lun Liao, Hong-Yu Liu, Helene Minyi Lu, Yen-Wen Yeh, Pin-Kuan Chang, Justin Yu Fan, Shih-Kang Micromachines (Basel) Article Point-of-care (POC) tests capable of individual health monitoring, transmission reduction, and contact tracing are especially important in a pandemic such as the coronavirus disease 2019 (COVID-19). We develop a disposable POC cartridge that can be mass produced to detect the SARS-CoV-2 N gene through real-time quantitative polymerase chain reaction (qPCR) based on digital microfluidics (DMF). Several critical parameters are studied and improved, including droplet volume consistency, temperature uniformity, and fluorescence intensity linearity on the designed DMF cartridge. The qPCR results showed high accuracy and efficiency for two primer-probe sets of N1 and N2 target regions of the SARS-CoV-2 N gene on the DMF cartridge. Having multiple droplet tracks for qPCR, the presented DMF cartridge can perform multiple tests and controls at once. MDPI 2022-01-27 /pmc/articles/PMC8874717/ /pubmed/35208320 http://dx.doi.org/10.3390/mi13020196 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ho, Kuan-Lun Liao, Hong-Yu Liu, Helene Minyi Lu, Yen-Wen Yeh, Pin-Kuan Chang, Justin Yu Fan, Shih-Kang Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title | Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title_full | Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title_fullStr | Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title_full_unstemmed | Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title_short | Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection |
title_sort | digital microfluidic qpcr cartridge for sars-cov-2 detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874717/ https://www.ncbi.nlm.nih.gov/pubmed/35208320 http://dx.doi.org/10.3390/mi13020196 |
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