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The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils

Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi,...

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Autores principales: Reis, Ana Paula Carvalho, Celestrino, Giovanna Azevedo, Igoa, Mariana Villas Bôas, Jesus, Thais Martins, França, Tábata Takahashi, Moreira, Daniel Valério Silva, Rigato, Paula Ordonhez, Sato, Paula Keiko, Condino-Neto, Antonio, Noronha, Irene L., Dias-Melicio, Luciane Alarcão, Lalwani, Pritesh Jaychand, Benard, Gil, Sousa, Maria Gloria Teixeira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874784/
https://www.ncbi.nlm.nih.gov/pubmed/35205902
http://dx.doi.org/10.3390/jof8020147
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author Reis, Ana Paula Carvalho
Celestrino, Giovanna Azevedo
Igoa, Mariana Villas Bôas
Jesus, Thais Martins
França, Tábata Takahashi
Moreira, Daniel Valério Silva
Rigato, Paula Ordonhez
Sato, Paula Keiko
Condino-Neto, Antonio
Noronha, Irene L.
Dias-Melicio, Luciane Alarcão
Lalwani, Pritesh Jaychand
Benard, Gil
Sousa, Maria Gloria Teixeira
author_facet Reis, Ana Paula Carvalho
Celestrino, Giovanna Azevedo
Igoa, Mariana Villas Bôas
Jesus, Thais Martins
França, Tábata Takahashi
Moreira, Daniel Valério Silva
Rigato, Paula Ordonhez
Sato, Paula Keiko
Condino-Neto, Antonio
Noronha, Irene L.
Dias-Melicio, Luciane Alarcão
Lalwani, Pritesh Jaychand
Benard, Gil
Sousa, Maria Gloria Teixeira
author_sort Reis, Ana Paula Carvalho
collection PubMed
description Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi, including Aspergillus, spp., Candida albicans, and Phialophora verrucosa. However, NET production in response to Trichophyton rubrum, the main etiologic agent of dermatophytosis, has yet to be studied. We demonstrated that human neutrophils produce NETs against different morphotypes of T. rubrum in a dose-dependent manner and NET formation is dependent on ROS production. In addition, ROS production by human neutrophils in response to T. rubrum is dependent on NADPH oxidase, but not on fungal viability. NETs mediated killing of T. rubrum. Collectively, these results demonstrate that T. rubrum was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological response to T. rubrum infection.
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spelling pubmed-88747842022-02-26 The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils Reis, Ana Paula Carvalho Celestrino, Giovanna Azevedo Igoa, Mariana Villas Bôas Jesus, Thais Martins França, Tábata Takahashi Moreira, Daniel Valério Silva Rigato, Paula Ordonhez Sato, Paula Keiko Condino-Neto, Antonio Noronha, Irene L. Dias-Melicio, Luciane Alarcão Lalwani, Pritesh Jaychand Benard, Gil Sousa, Maria Gloria Teixeira J Fungi (Basel) Article Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi, including Aspergillus, spp., Candida albicans, and Phialophora verrucosa. However, NET production in response to Trichophyton rubrum, the main etiologic agent of dermatophytosis, has yet to be studied. We demonstrated that human neutrophils produce NETs against different morphotypes of T. rubrum in a dose-dependent manner and NET formation is dependent on ROS production. In addition, ROS production by human neutrophils in response to T. rubrum is dependent on NADPH oxidase, but not on fungal viability. NETs mediated killing of T. rubrum. Collectively, these results demonstrate that T. rubrum was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological response to T. rubrum infection. MDPI 2022-01-31 /pmc/articles/PMC8874784/ /pubmed/35205902 http://dx.doi.org/10.3390/jof8020147 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reis, Ana Paula Carvalho
Celestrino, Giovanna Azevedo
Igoa, Mariana Villas Bôas
Jesus, Thais Martins
França, Tábata Takahashi
Moreira, Daniel Valério Silva
Rigato, Paula Ordonhez
Sato, Paula Keiko
Condino-Neto, Antonio
Noronha, Irene L.
Dias-Melicio, Luciane Alarcão
Lalwani, Pritesh Jaychand
Benard, Gil
Sousa, Maria Gloria Teixeira
The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title_full The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title_fullStr The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title_full_unstemmed The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title_short The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
title_sort dermatophyte trichophyton rubrum induces neutrophil extracellular traps release by human neutrophils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874784/
https://www.ncbi.nlm.nih.gov/pubmed/35205902
http://dx.doi.org/10.3390/jof8020147
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