No Impact of Biocontrol Agent’s Predation Cues on Development Time or Size of Surviving Aedes albopictus under Optimal Nutritional Availability

SIMPLE SUMMARY: Aedes albopictus is a highly invasive species of mosquito that can infect humans with chikungunya, dengue, yellow fever, and Zika. Within the next few decades, this mosquito species is predicted to invade South East England. Cyclopoid copepods are small crustaceans that have previously been used as biocontrol agents due to their high efficiency at killing small early instar mosquito larvae. We assessed the effect of Megacyclops viridis, a copepod species local to South East England, on the survival and traits of mosquito larvae exposed to these predators during the larger late instar stage. Our experiment was designed to measure the impact of copepod predation on both the development time and adult body size of Ae. albopictus. These traits can shape mosquito population dynamics and disease transmission. While we found that copepod attacks cause a small increase in late instar mortality, our methods did not detect a significant difference in either development time or size between the predator and control treatments. The lack of a strong sublethal effect on these traits supports the use of M. viridis copepods as biocontrol agents against Ae. albopictus in the UK. This information may be useful for guiding public health measures that aim to prevent outbreaks of mosquito-borne disease. ABSTRACT: Cyclopoid copepods have been applied successfully to limit populations of highly invasive Aedes albopictus mosquitoes that can transmit diseases of public health importance. However, there is concern that changes in certain mosquito traits, induced by exposure to copepod predation, might increase the risk of disease transmission. In this study, third instar Ae. albopictus larvae (focal individuals) were exposed to Megacyclops viridis predator cues associated with both the consumption of newly hatched mosquito larvae and attacks on focal individuals. The number of newly hatched larvae surrounding each focal larva was held constant to control for density effects on size, and the focal individual’s day of pupation and wing length were recorded for each replicate. Exposing late instar Ae. albopictus to predation decreased their chances of surviving to adulthood, and three focal larvae that died in the predator treatment showed signs of melanisation, indicative of wounding. Among surviving focal Ae. albopictus, no significant difference in either pupation day or wing length was observed due to copepod predation. The absence of significant sublethal impacts from M. viridis copepod predation on surviving later stage larvae in this analysis supports the use of M. viridis as a biocontrol agent against Ae. albopictus.