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Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka

Coronaviruses (CoV) are divided into the genera α-CoVs, β-CoVs, γ-CoVs and δ-CoVs. Of these, α-CoVs and β-CoVs are solely capable of causing infections in humans, resulting in mild to severe respiratory symptoms. Bats have been identified as natural reservoir hosts for CoVs belonging to these two ge...

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Autores principales: Muzeniek, Therese, Perera, Thejanee, Siriwardana, Sahan, Bas, Dilara, Kaplan, Fatimanur, Öruc, Mizgin, Becker-Ziaja, Beate, Perera, Inoka, Weerasena, Jagathpriya, Handunnetti, Shiroma, Schwarz, Franziska, Premawansa, Gayani, Premawansa, Sunil, Yapa, Wipula, Nitsche, Andreas, Kohl, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874963/
https://www.ncbi.nlm.nih.gov/pubmed/35215931
http://dx.doi.org/10.3390/v14020337
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author Muzeniek, Therese
Perera, Thejanee
Siriwardana, Sahan
Bas, Dilara
Kaplan, Fatimanur
Öruc, Mizgin
Becker-Ziaja, Beate
Perera, Inoka
Weerasena, Jagathpriya
Handunnetti, Shiroma
Schwarz, Franziska
Premawansa, Gayani
Premawansa, Sunil
Yapa, Wipula
Nitsche, Andreas
Kohl, Claudia
author_facet Muzeniek, Therese
Perera, Thejanee
Siriwardana, Sahan
Bas, Dilara
Kaplan, Fatimanur
Öruc, Mizgin
Becker-Ziaja, Beate
Perera, Inoka
Weerasena, Jagathpriya
Handunnetti, Shiroma
Schwarz, Franziska
Premawansa, Gayani
Premawansa, Sunil
Yapa, Wipula
Nitsche, Andreas
Kohl, Claudia
author_sort Muzeniek, Therese
collection PubMed
description Coronaviruses (CoV) are divided into the genera α-CoVs, β-CoVs, γ-CoVs and δ-CoVs. Of these, α-CoVs and β-CoVs are solely capable of causing infections in humans, resulting in mild to severe respiratory symptoms. Bats have been identified as natural reservoir hosts for CoVs belonging to these two genera. Consequently, research on bat populations, CoV prevalence in bats and genetic characterization of bat CoVs is of special interest to investigate the potential transmission risks. We present the genome sequence of a novel α-CoV strain detected in rectal swab samples of Miniopterus fuliginosus bats from a colony in the Wavul Galge cave (Koslanda, Sri Lanka). The novel strain is highly similar to Miniopterus bat coronavirus 1, an α-CoV located in the subgenus of Minunacoviruses. Phylogenetic reconstruction revealed a high identity of the novel strain to other α-CoVs derived from Miniopterus bats, while human-pathogenic α-CoV strains like HCoV-229E and HCoV-NL63 were more distantly related. Comparison with selected bat-related and human-pathogenic strains of the β-CoV genus showed low identities of ~40%. Analyses of the different genes on nucleotide and amino acid level revealed that the non-structural ORF1a/1b are more conserved among α-CoVs and β-CoVs, while there are higher variations in the structural proteins known to be important for host specificity. The novel strain was named batCoV/MinFul/2018/SriLanka and had a prevalence of 50% (66/130) in rectal swab samples and 58% (61/104) in feces samples that were collected from Miniopterus bats in Wavul Galge cave. Based on the differences between strain batCoV/MinFul/2018/SriLanka and human-pathogenic α-CoVs and β-CoVs, we conclude that there is a rather low transmission risk to humans. Further studies in the Wavul Galge cave and at other locations in Sri Lanka will give more detailed information about the prevalence of this virus.
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spelling pubmed-88749632022-02-26 Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka Muzeniek, Therese Perera, Thejanee Siriwardana, Sahan Bas, Dilara Kaplan, Fatimanur Öruc, Mizgin Becker-Ziaja, Beate Perera, Inoka Weerasena, Jagathpriya Handunnetti, Shiroma Schwarz, Franziska Premawansa, Gayani Premawansa, Sunil Yapa, Wipula Nitsche, Andreas Kohl, Claudia Viruses Article Coronaviruses (CoV) are divided into the genera α-CoVs, β-CoVs, γ-CoVs and δ-CoVs. Of these, α-CoVs and β-CoVs are solely capable of causing infections in humans, resulting in mild to severe respiratory symptoms. Bats have been identified as natural reservoir hosts for CoVs belonging to these two genera. Consequently, research on bat populations, CoV prevalence in bats and genetic characterization of bat CoVs is of special interest to investigate the potential transmission risks. We present the genome sequence of a novel α-CoV strain detected in rectal swab samples of Miniopterus fuliginosus bats from a colony in the Wavul Galge cave (Koslanda, Sri Lanka). The novel strain is highly similar to Miniopterus bat coronavirus 1, an α-CoV located in the subgenus of Minunacoviruses. Phylogenetic reconstruction revealed a high identity of the novel strain to other α-CoVs derived from Miniopterus bats, while human-pathogenic α-CoV strains like HCoV-229E and HCoV-NL63 were more distantly related. Comparison with selected bat-related and human-pathogenic strains of the β-CoV genus showed low identities of ~40%. Analyses of the different genes on nucleotide and amino acid level revealed that the non-structural ORF1a/1b are more conserved among α-CoVs and β-CoVs, while there are higher variations in the structural proteins known to be important for host specificity. The novel strain was named batCoV/MinFul/2018/SriLanka and had a prevalence of 50% (66/130) in rectal swab samples and 58% (61/104) in feces samples that were collected from Miniopterus bats in Wavul Galge cave. Based on the differences between strain batCoV/MinFul/2018/SriLanka and human-pathogenic α-CoVs and β-CoVs, we conclude that there is a rather low transmission risk to humans. Further studies in the Wavul Galge cave and at other locations in Sri Lanka will give more detailed information about the prevalence of this virus. MDPI 2022-02-07 /pmc/articles/PMC8874963/ /pubmed/35215931 http://dx.doi.org/10.3390/v14020337 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muzeniek, Therese
Perera, Thejanee
Siriwardana, Sahan
Bas, Dilara
Kaplan, Fatimanur
Öruc, Mizgin
Becker-Ziaja, Beate
Perera, Inoka
Weerasena, Jagathpriya
Handunnetti, Shiroma
Schwarz, Franziska
Premawansa, Gayani
Premawansa, Sunil
Yapa, Wipula
Nitsche, Andreas
Kohl, Claudia
Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title_full Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title_fullStr Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title_full_unstemmed Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title_short Full Genome of batCoV/MinFul/2018/SriLanka, a Novel Alpha-Coronavirus Detected in Miniopterus fuliginosus, Sri Lanka
title_sort full genome of batcov/minful/2018/srilanka, a novel alpha-coronavirus detected in miniopterus fuliginosus, sri lanka
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874963/
https://www.ncbi.nlm.nih.gov/pubmed/35215931
http://dx.doi.org/10.3390/v14020337
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