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Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides
Since the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of Streptococcus mutans, the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate the bacterial prote...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875007/ https://www.ncbi.nlm.nih.gov/pubmed/35208678 http://dx.doi.org/10.3390/microorganisms10020223 |
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author | Marin, Lina Maria Xiao, Yizhi Cury, Jaime Aparecido Siqueira, Walter Luiz |
author_facet | Marin, Lina Maria Xiao, Yizhi Cury, Jaime Aparecido Siqueira, Walter Luiz |
author_sort | Marin, Lina Maria |
collection | PubMed |
description | Since the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of Streptococcus mutans, the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate the bacterial proteome upon adherence. Single-component AEPs were formed onto hydroxyapatite (HAp) discs by incubating them with statherin, histatin-3, DR9, DR9-DR9, DR9-RR14, RR14, and parotid saliva. Then, the discs were inoculated with S. mutans UA159 and the bacteria were allowed to adhere for 2 h, 4 h, and 8 h (n = 12/treatment/time point). The number of bacteria adhered to the HAp discs was determined at each time point and analyzed by two-way ANOVA and Bonferroni tests. Cell-wall proteins were extracted from adhered, planktonic, and inoculum (baseline) bacteria and proteome profiles were obtained after a bottom-up proteomics approach. The number of adhered bacteria significantly increased over time, being the mean values obtained at 8 h, from highest to lowest, as follows: DR9-RR14 > statherin > RR14 = DR9-DR9 > DR9 = histatin3 > saliva (p < 0.05). Treatments modulated the bacterial proteome upon adherence. The findings suggested a potential use of our engineered peptide DR9-DR9 to control S. mutans biofilm development by reducing bacterial colonization. |
format | Online Article Text |
id | pubmed-8875007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88750072022-02-26 Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides Marin, Lina Maria Xiao, Yizhi Cury, Jaime Aparecido Siqueira, Walter Luiz Microorganisms Article Since the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of Streptococcus mutans, the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate the bacterial proteome upon adherence. Single-component AEPs were formed onto hydroxyapatite (HAp) discs by incubating them with statherin, histatin-3, DR9, DR9-DR9, DR9-RR14, RR14, and parotid saliva. Then, the discs were inoculated with S. mutans UA159 and the bacteria were allowed to adhere for 2 h, 4 h, and 8 h (n = 12/treatment/time point). The number of bacteria adhered to the HAp discs was determined at each time point and analyzed by two-way ANOVA and Bonferroni tests. Cell-wall proteins were extracted from adhered, planktonic, and inoculum (baseline) bacteria and proteome profiles were obtained after a bottom-up proteomics approach. The number of adhered bacteria significantly increased over time, being the mean values obtained at 8 h, from highest to lowest, as follows: DR9-RR14 > statherin > RR14 = DR9-DR9 > DR9 = histatin3 > saliva (p < 0.05). Treatments modulated the bacterial proteome upon adherence. The findings suggested a potential use of our engineered peptide DR9-DR9 to control S. mutans biofilm development by reducing bacterial colonization. MDPI 2022-01-20 /pmc/articles/PMC8875007/ /pubmed/35208678 http://dx.doi.org/10.3390/microorganisms10020223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marin, Lina Maria Xiao, Yizhi Cury, Jaime Aparecido Siqueira, Walter Luiz Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title | Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title_full | Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title_fullStr | Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title_full_unstemmed | Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title_short | Modulation of Streptococcus mutans Adherence to Hydroxyapatite by Engineered Salivary Peptides |
title_sort | modulation of streptococcus mutans adherence to hydroxyapatite by engineered salivary peptides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875007/ https://www.ncbi.nlm.nih.gov/pubmed/35208678 http://dx.doi.org/10.3390/microorganisms10020223 |
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