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Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity

Pathogenic superbugs are the root cause of untreatable complex infections with limited or no treatment options. These infections are becoming more common as clinical antibiotics have lost their effectiveness over time. Therefore, the development of novel antibacterial agents is urgently needed to co...

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Autores principales: Hazam, Prakash Kishore, Cheng, Chin-Cheng, Hsieh, Chu-Yi, Lin, Wen-Chun, Hsu, Po-Hsien, Chen, Te-Li, Lee, Yi-Tzu, Chen, Jyh-Yih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875074/
https://www.ncbi.nlm.nih.gov/pubmed/35216307
http://dx.doi.org/10.3390/ijms23042191
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author Hazam, Prakash Kishore
Cheng, Chin-Cheng
Hsieh, Chu-Yi
Lin, Wen-Chun
Hsu, Po-Hsien
Chen, Te-Li
Lee, Yi-Tzu
Chen, Jyh-Yih
author_facet Hazam, Prakash Kishore
Cheng, Chin-Cheng
Hsieh, Chu-Yi
Lin, Wen-Chun
Hsu, Po-Hsien
Chen, Te-Li
Lee, Yi-Tzu
Chen, Jyh-Yih
author_sort Hazam, Prakash Kishore
collection PubMed
description Pathogenic superbugs are the root cause of untreatable complex infections with limited or no treatment options. These infections are becoming more common as clinical antibiotics have lost their effectiveness over time. Therefore, the development of novel antibacterial agents is urgently needed to counter these microbes. Antimicrobial peptides (AMPs) are a viable treatment option due to their bactericidal potency against multiple microbial classes. AMPs are naturally selected physiological microbicidal agents that are found in all forms of organisms. In the present study, we developed two tilapia piscidin 2 (TP2)-based AMPs for antimicrobial application. Unlike the parent peptide, the redesigned peptides showed significant antimicrobial activity against multidrug-resistant bacterial species. These peptides also showed minimal cytotoxicity. In addition, they were significantly active in the presence of physiological salts, 50% human serum and elevated temperature. The designed peptides also showed synergistic activity when combined with clinical antibiotics. The current approach demonstrates a fruitful strategy for developing potential AMPs for antimicrobial application. Such AMPs have potential for progression to further trials and drug development investigations.
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spelling pubmed-88750742022-02-26 Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity Hazam, Prakash Kishore Cheng, Chin-Cheng Hsieh, Chu-Yi Lin, Wen-Chun Hsu, Po-Hsien Chen, Te-Li Lee, Yi-Tzu Chen, Jyh-Yih Int J Mol Sci Article Pathogenic superbugs are the root cause of untreatable complex infections with limited or no treatment options. These infections are becoming more common as clinical antibiotics have lost their effectiveness over time. Therefore, the development of novel antibacterial agents is urgently needed to counter these microbes. Antimicrobial peptides (AMPs) are a viable treatment option due to their bactericidal potency against multiple microbial classes. AMPs are naturally selected physiological microbicidal agents that are found in all forms of organisms. In the present study, we developed two tilapia piscidin 2 (TP2)-based AMPs for antimicrobial application. Unlike the parent peptide, the redesigned peptides showed significant antimicrobial activity against multidrug-resistant bacterial species. These peptides also showed minimal cytotoxicity. In addition, they were significantly active in the presence of physiological salts, 50% human serum and elevated temperature. The designed peptides also showed synergistic activity when combined with clinical antibiotics. The current approach demonstrates a fruitful strategy for developing potential AMPs for antimicrobial application. Such AMPs have potential for progression to further trials and drug development investigations. MDPI 2022-02-16 /pmc/articles/PMC8875074/ /pubmed/35216307 http://dx.doi.org/10.3390/ijms23042191 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hazam, Prakash Kishore
Cheng, Chin-Cheng
Hsieh, Chu-Yi
Lin, Wen-Chun
Hsu, Po-Hsien
Chen, Te-Li
Lee, Yi-Tzu
Chen, Jyh-Yih
Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title_full Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title_fullStr Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title_full_unstemmed Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title_short Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
title_sort development of bactericidal peptides against multidrug-resistant acinetobacter baumannii with enhanced stability and low toxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875074/
https://www.ncbi.nlm.nih.gov/pubmed/35216307
http://dx.doi.org/10.3390/ijms23042191
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