Cargando…
A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) with enhanced replication capability emerged in China and has become dominant epidemic strain since 2006. Up to now, the replication-regulated genes of PRRSV have not been fully clarified. Here, by swapping the genes or...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875199/ https://www.ncbi.nlm.nih.gov/pubmed/35215760 http://dx.doi.org/10.3390/v14020166 |
_version_ | 1784657860718755840 |
---|---|
author | Xiong, Junyao Cui, Xingyang Zhao, Kuan Wang, Qian Huang, Xinyi Li, Dongyan Yu, Fang Yang, Yongbo Liu, Di Tian, Zhijun Cai, Xuehui An, Tongqing |
author_facet | Xiong, Junyao Cui, Xingyang Zhao, Kuan Wang, Qian Huang, Xinyi Li, Dongyan Yu, Fang Yang, Yongbo Liu, Di Tian, Zhijun Cai, Xuehui An, Tongqing |
author_sort | Xiong, Junyao |
collection | PubMed |
description | Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) with enhanced replication capability emerged in China and has become dominant epidemic strain since 2006. Up to now, the replication-regulated genes of PRRSV have not been fully clarified. Here, by swapping the genes or elements between HP-PRRSV and classical PRRSV based on infectious clones, NSP1, NSP2, NSP7, NSP9 and 3′-UTR are found to contribute to the high replication efficiency of HP-PRRSV. Further study revealed that mutations at positions 117th or 119th in the 3′-UTR are significantly related to replication efficiency, and the nucleotide at position 120th is critical for viral rescue. The motif composed by 117–120th nucleotides was quite conservative within each lineage of PRRSV; mutations in the motif of HP-PRRSV and currently epidemic lineage 1 (L1) PRRSV showed higher synthesis ability of viral negative genomic RNA, suggesting that those mutations were beneficial for viral replication. RNA structure analysis revealed that this motif maybe involved into a pseudoknot in the 3′-UTR. The results discovered a novel motif, 117–120th nucleotide in the 3′-UTR, that is critical for replication of PRRSV-2, and mutations in the motif contribute to the enhanced replicative ability of HP-PRRSV or L1 PRRSV. Our findings will help to understand the molecular basis of PRRSV replication and find the potential factors resulting in an epidemic strain of PRRSV. |
format | Online Article Text |
id | pubmed-8875199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88751992022-02-26 A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV Xiong, Junyao Cui, Xingyang Zhao, Kuan Wang, Qian Huang, Xinyi Li, Dongyan Yu, Fang Yang, Yongbo Liu, Di Tian, Zhijun Cai, Xuehui An, Tongqing Viruses Article Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) with enhanced replication capability emerged in China and has become dominant epidemic strain since 2006. Up to now, the replication-regulated genes of PRRSV have not been fully clarified. Here, by swapping the genes or elements between HP-PRRSV and classical PRRSV based on infectious clones, NSP1, NSP2, NSP7, NSP9 and 3′-UTR are found to contribute to the high replication efficiency of HP-PRRSV. Further study revealed that mutations at positions 117th or 119th in the 3′-UTR are significantly related to replication efficiency, and the nucleotide at position 120th is critical for viral rescue. The motif composed by 117–120th nucleotides was quite conservative within each lineage of PRRSV; mutations in the motif of HP-PRRSV and currently epidemic lineage 1 (L1) PRRSV showed higher synthesis ability of viral negative genomic RNA, suggesting that those mutations were beneficial for viral replication. RNA structure analysis revealed that this motif maybe involved into a pseudoknot in the 3′-UTR. The results discovered a novel motif, 117–120th nucleotide in the 3′-UTR, that is critical for replication of PRRSV-2, and mutations in the motif contribute to the enhanced replicative ability of HP-PRRSV or L1 PRRSV. Our findings will help to understand the molecular basis of PRRSV replication and find the potential factors resulting in an epidemic strain of PRRSV. MDPI 2022-01-18 /pmc/articles/PMC8875199/ /pubmed/35215760 http://dx.doi.org/10.3390/v14020166 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xiong, Junyao Cui, Xingyang Zhao, Kuan Wang, Qian Huang, Xinyi Li, Dongyan Yu, Fang Yang, Yongbo Liu, Di Tian, Zhijun Cai, Xuehui An, Tongqing A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title | A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title_full | A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title_fullStr | A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title_full_unstemmed | A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title_short | A Novel Motif in the 3′-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV |
title_sort | novel motif in the 3′-utr of prrsv-2 is critical for viral multiplication and contributes to enhanced replication ability of highly pathogenic or l1 prrsv |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875199/ https://www.ncbi.nlm.nih.gov/pubmed/35215760 http://dx.doi.org/10.3390/v14020166 |
work_keys_str_mv | AT xiongjunyao anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT cuixingyang anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT zhaokuan anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT wangqian anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT huangxinyi anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT lidongyan anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT yufang anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT yangyongbo anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT liudi anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT tianzhijun anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT caixuehui anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT antongqing anovelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT xiongjunyao novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT cuixingyang novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT zhaokuan novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT wangqian novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT huangxinyi novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT lidongyan novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT yufang novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT yangyongbo novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT liudi novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT tianzhijun novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT caixuehui novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv AT antongqing novelmotifinthe3utrofprrsv2iscriticalforviralmultiplicationandcontributestoenhancedreplicationabilityofhighlypathogenicorl1prrsv |