Cargando…

Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences

Imperatorin, a naturally derived furanocoumarin, exerts promising neuropharmacological properties. Therefore, it might be applicable in the treatment of brain diseases such as depression. In the present project, we aimed to investigate the sex-dependent effects of imperatorin (1, 5, and 10 mg/kg) on...

Descripción completa

Detalles Bibliográficos
Autores principales: Kowalczyk, Joanna, Nakos-Bimpos, Modestos, Polissidis, Alexia, Dalla, Christina, Kokras, Nikolaos, Skalicka-Woźniak, Krystyna, Budzyńska, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875245/
https://www.ncbi.nlm.nih.gov/pubmed/35208969
http://dx.doi.org/10.3390/molecules27041179
_version_ 1784657871802204160
author Kowalczyk, Joanna
Nakos-Bimpos, Modestos
Polissidis, Alexia
Dalla, Christina
Kokras, Nikolaos
Skalicka-Woźniak, Krystyna
Budzyńska, Barbara
author_facet Kowalczyk, Joanna
Nakos-Bimpos, Modestos
Polissidis, Alexia
Dalla, Christina
Kokras, Nikolaos
Skalicka-Woźniak, Krystyna
Budzyńska, Barbara
author_sort Kowalczyk, Joanna
collection PubMed
description Imperatorin, a naturally derived furanocoumarin, exerts promising neuropharmacological properties. Therefore, it might be applicable in the treatment of brain diseases such as depression. In the present project, we aimed to investigate the sex-dependent effects of imperatorin (1, 5, and 10 mg/kg) on behavior and neurochemistry associated with antidepressant effects. The depressive-like behaviors of male and female Swiss mice were investigated in a forced swim test (FST). Subsequently, High-Performance Liquid Chromatography (HPLC) was used to evaluate the level of serotonin, its metabolite, 5-HIAA, and noradrenaline, in mouse brains. The study revealed that only males responded to imperatorin (1 and 5 mg/kg) treatment and caused an antidepressant effect, such as with respect to depressive-like behaviors, lowering immobility time and increasing immobility latency. The HPLC analysis demonstrated that serotonin levels in the prefrontal cortex of females decreased with the middle dose of imperatorin (5 mg/kg), while in the male prefrontal cortex, the lower dose (1 mg/kg) boosted serotonin levels. There were no evident changes observed with respect to noradrenaline and serotonin metabolite levels in the male hippocampus. To conclude, we propose that imperatorin has antidepressant potential, seemingly only in males, influencing brain serotonin level, but the direct mechanism of action requires further investigation.
format Online
Article
Text
id pubmed-8875245
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88752452022-02-26 Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences Kowalczyk, Joanna Nakos-Bimpos, Modestos Polissidis, Alexia Dalla, Christina Kokras, Nikolaos Skalicka-Woźniak, Krystyna Budzyńska, Barbara Molecules Article Imperatorin, a naturally derived furanocoumarin, exerts promising neuropharmacological properties. Therefore, it might be applicable in the treatment of brain diseases such as depression. In the present project, we aimed to investigate the sex-dependent effects of imperatorin (1, 5, and 10 mg/kg) on behavior and neurochemistry associated with antidepressant effects. The depressive-like behaviors of male and female Swiss mice were investigated in a forced swim test (FST). Subsequently, High-Performance Liquid Chromatography (HPLC) was used to evaluate the level of serotonin, its metabolite, 5-HIAA, and noradrenaline, in mouse brains. The study revealed that only males responded to imperatorin (1 and 5 mg/kg) treatment and caused an antidepressant effect, such as with respect to depressive-like behaviors, lowering immobility time and increasing immobility latency. The HPLC analysis demonstrated that serotonin levels in the prefrontal cortex of females decreased with the middle dose of imperatorin (5 mg/kg), while in the male prefrontal cortex, the lower dose (1 mg/kg) boosted serotonin levels. There were no evident changes observed with respect to noradrenaline and serotonin metabolite levels in the male hippocampus. To conclude, we propose that imperatorin has antidepressant potential, seemingly only in males, influencing brain serotonin level, but the direct mechanism of action requires further investigation. MDPI 2022-02-10 /pmc/articles/PMC8875245/ /pubmed/35208969 http://dx.doi.org/10.3390/molecules27041179 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kowalczyk, Joanna
Nakos-Bimpos, Modestos
Polissidis, Alexia
Dalla, Christina
Kokras, Nikolaos
Skalicka-Woźniak, Krystyna
Budzyńska, Barbara
Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title_full Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title_fullStr Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title_full_unstemmed Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title_short Imperatorin Influences Depressive-like Behaviors: A Preclinical Study on Behavioral and Neurochemical Sex Differences
title_sort imperatorin influences depressive-like behaviors: a preclinical study on behavioral and neurochemical sex differences
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875245/
https://www.ncbi.nlm.nih.gov/pubmed/35208969
http://dx.doi.org/10.3390/molecules27041179
work_keys_str_mv AT kowalczykjoanna imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT nakosbimposmodestos imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT polissidisalexia imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT dallachristina imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT kokrasnikolaos imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT skalickawozniakkrystyna imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences
AT budzynskabarbara imperatorininfluencesdepressivelikebehaviorsapreclinicalstudyonbehavioralandneurochemicalsexdifferences