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A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway

Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were de...

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Detalles Bibliográficos
Autores principales: Li, Chunlei, Zhang, Sirui, Zhu, Jianhua, Huang, Weijuan, Luo, Yuanyuan, Shi, Hui, Yu, Dongbo, Chen, Liguo, Song, Liyan, Yu, Rongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875476/
https://www.ncbi.nlm.nih.gov/pubmed/35200639
http://dx.doi.org/10.3390/md20020110
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author Li, Chunlei
Zhang, Sirui
Zhu, Jianhua
Huang, Weijuan
Luo, Yuanyuan
Shi, Hui
Yu, Dongbo
Chen, Liguo
Song, Liyan
Yu, Rongmin
author_facet Li, Chunlei
Zhang, Sirui
Zhu, Jianhua
Huang, Weijuan
Luo, Yuanyuan
Shi, Hui
Yu, Dongbo
Chen, Liguo
Song, Liyan
Yu, Rongmin
author_sort Li, Chunlei
collection PubMed
description Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were determined by tandem mass de novo sequencing and circular dichroism spectroscopy, respectively. P6 markedly inhibited cell proliferation and colony formation, and induced apoptosis in CRC cells. Mechanistically, transcriptomics analysis and a serial functional evaluation showed that P6 induced colon cancer cell apoptosis through the activation of the p38-MAPK signaling pathway. Moreover, it was demonstrated that P6 exhibited antitumor effects in a tumor xenograft model, and induced cell cycle arrest in CRC cells in a concentration-dependent mode. These findings provide the first line of indication that P6 could be a potential therapeutic agent for CRC treatment.
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spelling pubmed-88754762022-02-26 A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway Li, Chunlei Zhang, Sirui Zhu, Jianhua Huang, Weijuan Luo, Yuanyuan Shi, Hui Yu, Dongbo Chen, Liguo Song, Liyan Yu, Rongmin Mar Drugs Article Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were determined by tandem mass de novo sequencing and circular dichroism spectroscopy, respectively. P6 markedly inhibited cell proliferation and colony formation, and induced apoptosis in CRC cells. Mechanistically, transcriptomics analysis and a serial functional evaluation showed that P6 induced colon cancer cell apoptosis through the activation of the p38-MAPK signaling pathway. Moreover, it was demonstrated that P6 exhibited antitumor effects in a tumor xenograft model, and induced cell cycle arrest in CRC cells in a concentration-dependent mode. These findings provide the first line of indication that P6 could be a potential therapeutic agent for CRC treatment. MDPI 2022-01-29 /pmc/articles/PMC8875476/ /pubmed/35200639 http://dx.doi.org/10.3390/md20020110 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Chunlei
Zhang, Sirui
Zhu, Jianhua
Huang, Weijuan
Luo, Yuanyuan
Shi, Hui
Yu, Dongbo
Chen, Liguo
Song, Liyan
Yu, Rongmin
A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title_full A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title_fullStr A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title_full_unstemmed A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title_short A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
title_sort novel peptide derived from arca inflata induces apoptosis in colorectal cancer cells through mitochondria and the p38 mapk pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875476/
https://www.ncbi.nlm.nih.gov/pubmed/35200639
http://dx.doi.org/10.3390/md20020110
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