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A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway
Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were de...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875476/ https://www.ncbi.nlm.nih.gov/pubmed/35200639 http://dx.doi.org/10.3390/md20020110 |
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author | Li, Chunlei Zhang, Sirui Zhu, Jianhua Huang, Weijuan Luo, Yuanyuan Shi, Hui Yu, Dongbo Chen, Liguo Song, Liyan Yu, Rongmin |
author_facet | Li, Chunlei Zhang, Sirui Zhu, Jianhua Huang, Weijuan Luo, Yuanyuan Shi, Hui Yu, Dongbo Chen, Liguo Song, Liyan Yu, Rongmin |
author_sort | Li, Chunlei |
collection | PubMed |
description | Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were determined by tandem mass de novo sequencing and circular dichroism spectroscopy, respectively. P6 markedly inhibited cell proliferation and colony formation, and induced apoptosis in CRC cells. Mechanistically, transcriptomics analysis and a serial functional evaluation showed that P6 induced colon cancer cell apoptosis through the activation of the p38-MAPK signaling pathway. Moreover, it was demonstrated that P6 exhibited antitumor effects in a tumor xenograft model, and induced cell cycle arrest in CRC cells in a concentration-dependent mode. These findings provide the first line of indication that P6 could be a potential therapeutic agent for CRC treatment. |
format | Online Article Text |
id | pubmed-8875476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88754762022-02-26 A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway Li, Chunlei Zhang, Sirui Zhu, Jianhua Huang, Weijuan Luo, Yuanyuan Shi, Hui Yu, Dongbo Chen, Liguo Song, Liyan Yu, Rongmin Mar Drugs Article Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were determined by tandem mass de novo sequencing and circular dichroism spectroscopy, respectively. P6 markedly inhibited cell proliferation and colony formation, and induced apoptosis in CRC cells. Mechanistically, transcriptomics analysis and a serial functional evaluation showed that P6 induced colon cancer cell apoptosis through the activation of the p38-MAPK signaling pathway. Moreover, it was demonstrated that P6 exhibited antitumor effects in a tumor xenograft model, and induced cell cycle arrest in CRC cells in a concentration-dependent mode. These findings provide the first line of indication that P6 could be a potential therapeutic agent for CRC treatment. MDPI 2022-01-29 /pmc/articles/PMC8875476/ /pubmed/35200639 http://dx.doi.org/10.3390/md20020110 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Chunlei Zhang, Sirui Zhu, Jianhua Huang, Weijuan Luo, Yuanyuan Shi, Hui Yu, Dongbo Chen, Liguo Song, Liyan Yu, Rongmin A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title | A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title_full | A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title_fullStr | A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title_full_unstemmed | A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title_short | A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway |
title_sort | novel peptide derived from arca inflata induces apoptosis in colorectal cancer cells through mitochondria and the p38 mapk pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875476/ https://www.ncbi.nlm.nih.gov/pubmed/35200639 http://dx.doi.org/10.3390/md20020110 |
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