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Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population
Despite the high efficacy of the BNT162b2 vaccine in the general population, data on its immunogenicity among frail elderly individuals are limited. Recently, levels of anti-SARS-CoV-2 spike IgG antibodies and serum neutralization titers were confirmed as good immune markers of protection against th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875521/ https://www.ncbi.nlm.nih.gov/pubmed/35214717 http://dx.doi.org/10.3390/vaccines10020260 |
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author | Witkowski, Wojciech Gerlo, Sarah De Smet, Evelien Wejda, Magdalena Acar, Delphine Callens, Steven Heytens, Stefan Padalko, Elizaveta Vercruysse, Hanne Cools, Piet Vandekerckhove, Linos |
author_facet | Witkowski, Wojciech Gerlo, Sarah De Smet, Evelien Wejda, Magdalena Acar, Delphine Callens, Steven Heytens, Stefan Padalko, Elizaveta Vercruysse, Hanne Cools, Piet Vandekerckhove, Linos |
author_sort | Witkowski, Wojciech |
collection | PubMed |
description | Despite the high efficacy of the BNT162b2 vaccine in the general population, data on its immunogenicity among frail elderly individuals are limited. Recently, levels of anti-SARS-CoV-2 spike IgG antibodies and serum neutralization titers were confirmed as good immune markers of protection against the virus, with evidence showing a reverse correlation between these two parameters and susceptibility to infection. Here we analyzed sera from 138 nursing home residents (median age of 88.9 years) and 312 nursing home staff (median age of 50.7 years) to determine the humoral response to two doses of the BNT162b2 vaccine, and found markedly decreased serum anti-spike antibody levels and neutralization titers in the nursing home resident (NHR) group, with over 11% non-responders compared to only 1.3% among the controls. Moreover, three months post-vaccination, a significant decrease in antibody titers was observed in COVID-19-naive nursing home residents. Subsequent flow cytometry and interferon gamma secretion analyses indicated that antibody non-responders among NHRs also failed to mount cellular responses. The presented data emphasize that additional measures are needed in the population of frail elderly individuals. Given the high proportion of non-responders among NHRs, continued monitoring should be considered in this group. |
format | Online Article Text |
id | pubmed-8875521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88755212022-02-26 Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population Witkowski, Wojciech Gerlo, Sarah De Smet, Evelien Wejda, Magdalena Acar, Delphine Callens, Steven Heytens, Stefan Padalko, Elizaveta Vercruysse, Hanne Cools, Piet Vandekerckhove, Linos Vaccines (Basel) Article Despite the high efficacy of the BNT162b2 vaccine in the general population, data on its immunogenicity among frail elderly individuals are limited. Recently, levels of anti-SARS-CoV-2 spike IgG antibodies and serum neutralization titers were confirmed as good immune markers of protection against the virus, with evidence showing a reverse correlation between these two parameters and susceptibility to infection. Here we analyzed sera from 138 nursing home residents (median age of 88.9 years) and 312 nursing home staff (median age of 50.7 years) to determine the humoral response to two doses of the BNT162b2 vaccine, and found markedly decreased serum anti-spike antibody levels and neutralization titers in the nursing home resident (NHR) group, with over 11% non-responders compared to only 1.3% among the controls. Moreover, three months post-vaccination, a significant decrease in antibody titers was observed in COVID-19-naive nursing home residents. Subsequent flow cytometry and interferon gamma secretion analyses indicated that antibody non-responders among NHRs also failed to mount cellular responses. The presented data emphasize that additional measures are needed in the population of frail elderly individuals. Given the high proportion of non-responders among NHRs, continued monitoring should be considered in this group. MDPI 2022-02-08 /pmc/articles/PMC8875521/ /pubmed/35214717 http://dx.doi.org/10.3390/vaccines10020260 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Witkowski, Wojciech Gerlo, Sarah De Smet, Evelien Wejda, Magdalena Acar, Delphine Callens, Steven Heytens, Stefan Padalko, Elizaveta Vercruysse, Hanne Cools, Piet Vandekerckhove, Linos Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title | Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title_full | Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title_fullStr | Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title_full_unstemmed | Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title_short | Humoral and Cellular Responses to COVID-19 Vaccination Indicate the Need for Post-Vaccination Testing in Frail Population |
title_sort | humoral and cellular responses to covid-19 vaccination indicate the need for post-vaccination testing in frail population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875521/ https://www.ncbi.nlm.nih.gov/pubmed/35214717 http://dx.doi.org/10.3390/vaccines10020260 |
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