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Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and su...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875563/ https://www.ncbi.nlm.nih.gov/pubmed/35216211 http://dx.doi.org/10.3390/ijms23042100 |
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author | Gabanella, Francesca Barbato, Christian Corbi, Nicoletta Fiore, Marco Petrella, Carla de Vincentiis, Marco Greco, Antonio Ferraguti, Giampiero Corsi, Alessandro Ralli, Massimo Pecorella, Irene Di Gioia, Cira Pecorini, Francesco Brunelli, Roberto Passananti, Claudio Minni, Antonio Di Certo, Maria Grazia |
author_facet | Gabanella, Francesca Barbato, Christian Corbi, Nicoletta Fiore, Marco Petrella, Carla de Vincentiis, Marco Greco, Antonio Ferraguti, Giampiero Corsi, Alessandro Ralli, Massimo Pecorella, Irene Di Gioia, Cira Pecorini, Francesco Brunelli, Roberto Passananti, Claudio Minni, Antonio Di Certo, Maria Grazia |
author_sort | Gabanella, Francesca |
collection | PubMed |
description | The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal–fetal interface. |
format | Online Article Text |
id | pubmed-8875563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88755632022-02-26 Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta Gabanella, Francesca Barbato, Christian Corbi, Nicoletta Fiore, Marco Petrella, Carla de Vincentiis, Marco Greco, Antonio Ferraguti, Giampiero Corsi, Alessandro Ralli, Massimo Pecorella, Irene Di Gioia, Cira Pecorini, Francesco Brunelli, Roberto Passananti, Claudio Minni, Antonio Di Certo, Maria Grazia Int J Mol Sci Communication The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal–fetal interface. MDPI 2022-02-14 /pmc/articles/PMC8875563/ /pubmed/35216211 http://dx.doi.org/10.3390/ijms23042100 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Gabanella, Francesca Barbato, Christian Corbi, Nicoletta Fiore, Marco Petrella, Carla de Vincentiis, Marco Greco, Antonio Ferraguti, Giampiero Corsi, Alessandro Ralli, Massimo Pecorella, Irene Di Gioia, Cira Pecorini, Francesco Brunelli, Roberto Passananti, Claudio Minni, Antonio Di Certo, Maria Grazia Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title | Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title_full | Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title_fullStr | Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title_full_unstemmed | Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title_short | Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta |
title_sort | exploring mitochondrial localization of sars-cov-2 rna by padlock assay: a pilot study in human placenta |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875563/ https://www.ncbi.nlm.nih.gov/pubmed/35216211 http://dx.doi.org/10.3390/ijms23042100 |
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