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Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta

The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and su...

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Autores principales: Gabanella, Francesca, Barbato, Christian, Corbi, Nicoletta, Fiore, Marco, Petrella, Carla, de Vincentiis, Marco, Greco, Antonio, Ferraguti, Giampiero, Corsi, Alessandro, Ralli, Massimo, Pecorella, Irene, Di Gioia, Cira, Pecorini, Francesco, Brunelli, Roberto, Passananti, Claudio, Minni, Antonio, Di Certo, Maria Grazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875563/
https://www.ncbi.nlm.nih.gov/pubmed/35216211
http://dx.doi.org/10.3390/ijms23042100
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author Gabanella, Francesca
Barbato, Christian
Corbi, Nicoletta
Fiore, Marco
Petrella, Carla
de Vincentiis, Marco
Greco, Antonio
Ferraguti, Giampiero
Corsi, Alessandro
Ralli, Massimo
Pecorella, Irene
Di Gioia, Cira
Pecorini, Francesco
Brunelli, Roberto
Passananti, Claudio
Minni, Antonio
Di Certo, Maria Grazia
author_facet Gabanella, Francesca
Barbato, Christian
Corbi, Nicoletta
Fiore, Marco
Petrella, Carla
de Vincentiis, Marco
Greco, Antonio
Ferraguti, Giampiero
Corsi, Alessandro
Ralli, Massimo
Pecorella, Irene
Di Gioia, Cira
Pecorini, Francesco
Brunelli, Roberto
Passananti, Claudio
Minni, Antonio
Di Certo, Maria Grazia
author_sort Gabanella, Francesca
collection PubMed
description The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal–fetal interface.
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spelling pubmed-88755632022-02-26 Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta Gabanella, Francesca Barbato, Christian Corbi, Nicoletta Fiore, Marco Petrella, Carla de Vincentiis, Marco Greco, Antonio Ferraguti, Giampiero Corsi, Alessandro Ralli, Massimo Pecorella, Irene Di Gioia, Cira Pecorini, Francesco Brunelli, Roberto Passananti, Claudio Minni, Antonio Di Certo, Maria Grazia Int J Mol Sci Communication The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal–fetal interface. MDPI 2022-02-14 /pmc/articles/PMC8875563/ /pubmed/35216211 http://dx.doi.org/10.3390/ijms23042100 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gabanella, Francesca
Barbato, Christian
Corbi, Nicoletta
Fiore, Marco
Petrella, Carla
de Vincentiis, Marco
Greco, Antonio
Ferraguti, Giampiero
Corsi, Alessandro
Ralli, Massimo
Pecorella, Irene
Di Gioia, Cira
Pecorini, Francesco
Brunelli, Roberto
Passananti, Claudio
Minni, Antonio
Di Certo, Maria Grazia
Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title_full Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title_fullStr Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title_full_unstemmed Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title_short Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta
title_sort exploring mitochondrial localization of sars-cov-2 rna by padlock assay: a pilot study in human placenta
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875563/
https://www.ncbi.nlm.nih.gov/pubmed/35216211
http://dx.doi.org/10.3390/ijms23042100
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