Longer mtDNA Fragments Provide a Better Insight into the Genetic Diversity of the Sycamore Lace Bug, Corythucha ciliata (Say, 1832) (Tingidae, Hemiptera), Both in Its Native and Invaded Areas

SIMPLE SUMMARY: The sycamore lace bug (Corythucha ciliata Say, 1832) is one of the most abundant and widespread pests on plane trees (Platanus spp.) across the globe. The native range of the species is in North America, but it has been introduced to Europe (1964), South America (1985), Asia (1995), Australia (2006), and Africa (2014). To understand the genetic background behind this successful colonisation, we analysed a fragment (1356 bp) of the mitochondrial DNA. The 327 individuals revealed 17 haplotypes forming two separated groups. One group includes North American and Japanese individuals, while the other group comprises North American, European, and Asian individuals. We found a much higher genetic diversity in the native area (North America, 12 haplotypes) than in the invaded areas (Europe, five and Asia, four). The longer DNA fragment provided detailed information about the genetic structure of the species both in its native range and in the invaded areas, but the shorter DNA fragment could not provide a clear link between the genetic variation and the geographic origin. ABSTRACT: The sycamore lace bug (Corythucha ciliata Say, 1832) is of North American origin, but after its introduction to Europe (1964), South America (1985), Asia (1995), Australia (2006), and Africa (2014), it became an abundant and widespread pest on plane (Platanus spp.) trees. We analysed a 1356 bp long fragment of the mtDNA (COI gene) of 327 sycamore lace bug individuals from 38 geographic locations from Europe, Asia, and North America. Seventeen haplotypes (17 HTs) were detected. C. ciliata populations from North America exhibited higher haplotype diversity (12 HTs) than populations from Europe (6 HTs), Asia (4 HTs), or Japan (2 HTs). The haplotypes formed two haplogroups separated by at least seven mutation steps. One of these mutation steps includes HTs from North America and Japan. Another includes HTs from North America, Europe, and Asia. Haplotypes from Asia Minor, the Caucasus, and Central Asia are linked to haplotypes from Europe, while haplotypes found in Japan are linked to haplotypes found in North America only. The incorporation of published data from the GenBank into our dataset (altogether 517 individuals from 57 locations, but only 546 bp long fragment of the mtDNA) did not show any structure according to the geographic origin of the individuals.