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Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma

Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in...

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Detalles Bibliográficos
Autores principales: Shved, Natalia, Egorova, Anna, Osinovskaya, Natalia, Kiselev, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875914/
https://www.ncbi.nlm.nih.gov/pubmed/35200532
http://dx.doi.org/10.3390/mps5010016
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author Shved, Natalia
Egorova, Anna
Osinovskaya, Natalia
Kiselev, Anton
author_facet Shved, Natalia
Egorova, Anna
Osinovskaya, Natalia
Kiselev, Anton
author_sort Shved, Natalia
collection PubMed
description Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested.
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spelling pubmed-88759142022-02-26 Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma Shved, Natalia Egorova, Anna Osinovskaya, Natalia Kiselev, Anton Methods Protoc Protocol Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested. MDPI 2022-02-06 /pmc/articles/PMC8875914/ /pubmed/35200532 http://dx.doi.org/10.3390/mps5010016 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Shved, Natalia
Egorova, Anna
Osinovskaya, Natalia
Kiselev, Anton
Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title_full Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title_fullStr Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title_full_unstemmed Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title_short Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
title_sort development of primary monolayer cell model and organotypic model of uterine leiomyoma
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875914/
https://www.ncbi.nlm.nih.gov/pubmed/35200532
http://dx.doi.org/10.3390/mps5010016
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