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Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875914/ https://www.ncbi.nlm.nih.gov/pubmed/35200532 http://dx.doi.org/10.3390/mps5010016 |
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author | Shved, Natalia Egorova, Anna Osinovskaya, Natalia Kiselev, Anton |
author_facet | Shved, Natalia Egorova, Anna Osinovskaya, Natalia Kiselev, Anton |
author_sort | Shved, Natalia |
collection | PubMed |
description | Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested. |
format | Online Article Text |
id | pubmed-8875914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88759142022-02-26 Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma Shved, Natalia Egorova, Anna Osinovskaya, Natalia Kiselev, Anton Methods Protoc Protocol Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested. MDPI 2022-02-06 /pmc/articles/PMC8875914/ /pubmed/35200532 http://dx.doi.org/10.3390/mps5010016 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Shved, Natalia Egorova, Anna Osinovskaya, Natalia Kiselev, Anton Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title_full | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title_fullStr | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title_full_unstemmed | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title_short | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
title_sort | development of primary monolayer cell model and organotypic model of uterine leiomyoma |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875914/ https://www.ncbi.nlm.nih.gov/pubmed/35200532 http://dx.doi.org/10.3390/mps5010016 |
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