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The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to ass...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876135/ https://www.ncbi.nlm.nih.gov/pubmed/35216181 http://dx.doi.org/10.3390/ijms23042066 |
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author | Mancino, Davide Kharouf, Naji Scavello, Francesco Hellé, Sophie Salloum-Yared, Fouad Mutschler, Angela Mathieu, Eric Lavalle, Philippe Metz-Boutigue, Marie-Hélène Haïkel, Youssef |
author_facet | Mancino, Davide Kharouf, Naji Scavello, Francesco Hellé, Sophie Salloum-Yared, Fouad Mutschler, Angela Mathieu, Eric Lavalle, Philippe Metz-Boutigue, Marie-Hélène Haïkel, Youssef |
author_sort | Mancino, Davide |
collection | PubMed |
description | Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to assess the effectiveness against Candida species of different Catestatin-derived peptides, as well as the combined effect with serum albumin. Among Catestatin-derived peptides, the most active against sensitive and resistant strains of C. albicans, C. tropicalis and C. glabrata was the D-isomer of Cateslytin (D-bCtl) whereas the efficiency of the L-isomer (L-bCtl) significantly decreases against C. glabrata strains. Images obtained by transmission electron microscopy clearly demonstrated fungal membrane lysis and the leakage of the intracellular material induced by the L-bCtl and D-bCtl peptides. The possible synergistic effect of albumin on Catestatin-derived peptides activity was investigated too. Our finding showed that bovine serum albumin (BSA) when combined with the L- isomer of Catestatin (L-bCts) had a synergistic effect against Candida albicans especially at low concentrations of BSA; however, no synergistic effect was detected when BSA interacted with L-bCtl, suggesting the importance of the C-terminal end of L-bCts (GPGLQL) for the interaction with BSA. In this context in vitro D-bCtl, as well as the combination of BSA with L-bCts are potential candidates for the development of new antifungal drugs for the treatment of oral candidosis due to Candida and non-Candida albicans, without detrimental side effects. |
format | Online Article Text |
id | pubmed-8876135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88761352022-02-26 The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis Mancino, Davide Kharouf, Naji Scavello, Francesco Hellé, Sophie Salloum-Yared, Fouad Mutschler, Angela Mathieu, Eric Lavalle, Philippe Metz-Boutigue, Marie-Hélène Haïkel, Youssef Int J Mol Sci Article Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to assess the effectiveness against Candida species of different Catestatin-derived peptides, as well as the combined effect with serum albumin. Among Catestatin-derived peptides, the most active against sensitive and resistant strains of C. albicans, C. tropicalis and C. glabrata was the D-isomer of Cateslytin (D-bCtl) whereas the efficiency of the L-isomer (L-bCtl) significantly decreases against C. glabrata strains. Images obtained by transmission electron microscopy clearly demonstrated fungal membrane lysis and the leakage of the intracellular material induced by the L-bCtl and D-bCtl peptides. The possible synergistic effect of albumin on Catestatin-derived peptides activity was investigated too. Our finding showed that bovine serum albumin (BSA) when combined with the L- isomer of Catestatin (L-bCts) had a synergistic effect against Candida albicans especially at low concentrations of BSA; however, no synergistic effect was detected when BSA interacted with L-bCtl, suggesting the importance of the C-terminal end of L-bCts (GPGLQL) for the interaction with BSA. In this context in vitro D-bCtl, as well as the combination of BSA with L-bCts are potential candidates for the development of new antifungal drugs for the treatment of oral candidosis due to Candida and non-Candida albicans, without detrimental side effects. MDPI 2022-02-13 /pmc/articles/PMC8876135/ /pubmed/35216181 http://dx.doi.org/10.3390/ijms23042066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mancino, Davide Kharouf, Naji Scavello, Francesco Hellé, Sophie Salloum-Yared, Fouad Mutschler, Angela Mathieu, Eric Lavalle, Philippe Metz-Boutigue, Marie-Hélène Haïkel, Youssef The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title | The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title_full | The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title_fullStr | The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title_full_unstemmed | The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title_short | The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis |
title_sort | catestatin-derived peptides are new actors to fight the development of oral candidosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876135/ https://www.ncbi.nlm.nih.gov/pubmed/35216181 http://dx.doi.org/10.3390/ijms23042066 |
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