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The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis

Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to ass...

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Autores principales: Mancino, Davide, Kharouf, Naji, Scavello, Francesco, Hellé, Sophie, Salloum-Yared, Fouad, Mutschler, Angela, Mathieu, Eric, Lavalle, Philippe, Metz-Boutigue, Marie-Hélène, Haïkel, Youssef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876135/
https://www.ncbi.nlm.nih.gov/pubmed/35216181
http://dx.doi.org/10.3390/ijms23042066
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author Mancino, Davide
Kharouf, Naji
Scavello, Francesco
Hellé, Sophie
Salloum-Yared, Fouad
Mutschler, Angela
Mathieu, Eric
Lavalle, Philippe
Metz-Boutigue, Marie-Hélène
Haïkel, Youssef
author_facet Mancino, Davide
Kharouf, Naji
Scavello, Francesco
Hellé, Sophie
Salloum-Yared, Fouad
Mutschler, Angela
Mathieu, Eric
Lavalle, Philippe
Metz-Boutigue, Marie-Hélène
Haïkel, Youssef
author_sort Mancino, Davide
collection PubMed
description Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to assess the effectiveness against Candida species of different Catestatin-derived peptides, as well as the combined effect with serum albumin. Among Catestatin-derived peptides, the most active against sensitive and resistant strains of C. albicans, C. tropicalis and C. glabrata was the D-isomer of Cateslytin (D-bCtl) whereas the efficiency of the L-isomer (L-bCtl) significantly decreases against C. glabrata strains. Images obtained by transmission electron microscopy clearly demonstrated fungal membrane lysis and the leakage of the intracellular material induced by the L-bCtl and D-bCtl peptides. The possible synergistic effect of albumin on Catestatin-derived peptides activity was investigated too. Our finding showed that bovine serum albumin (BSA) when combined with the L- isomer of Catestatin (L-bCts) had a synergistic effect against Candida albicans especially at low concentrations of BSA; however, no synergistic effect was detected when BSA interacted with L-bCtl, suggesting the importance of the C-terminal end of L-bCts (GPGLQL) for the interaction with BSA. In this context in vitro D-bCtl, as well as the combination of BSA with L-bCts are potential candidates for the development of new antifungal drugs for the treatment of oral candidosis due to Candida and non-Candida albicans, without detrimental side effects.
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spelling pubmed-88761352022-02-26 The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis Mancino, Davide Kharouf, Naji Scavello, Francesco Hellé, Sophie Salloum-Yared, Fouad Mutschler, Angela Mathieu, Eric Lavalle, Philippe Metz-Boutigue, Marie-Hélène Haïkel, Youssef Int J Mol Sci Article Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to assess the effectiveness against Candida species of different Catestatin-derived peptides, as well as the combined effect with serum albumin. Among Catestatin-derived peptides, the most active against sensitive and resistant strains of C. albicans, C. tropicalis and C. glabrata was the D-isomer of Cateslytin (D-bCtl) whereas the efficiency of the L-isomer (L-bCtl) significantly decreases against C. glabrata strains. Images obtained by transmission electron microscopy clearly demonstrated fungal membrane lysis and the leakage of the intracellular material induced by the L-bCtl and D-bCtl peptides. The possible synergistic effect of albumin on Catestatin-derived peptides activity was investigated too. Our finding showed that bovine serum albumin (BSA) when combined with the L- isomer of Catestatin (L-bCts) had a synergistic effect against Candida albicans especially at low concentrations of BSA; however, no synergistic effect was detected when BSA interacted with L-bCtl, suggesting the importance of the C-terminal end of L-bCts (GPGLQL) for the interaction with BSA. In this context in vitro D-bCtl, as well as the combination of BSA with L-bCts are potential candidates for the development of new antifungal drugs for the treatment of oral candidosis due to Candida and non-Candida albicans, without detrimental side effects. MDPI 2022-02-13 /pmc/articles/PMC8876135/ /pubmed/35216181 http://dx.doi.org/10.3390/ijms23042066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mancino, Davide
Kharouf, Naji
Scavello, Francesco
Hellé, Sophie
Salloum-Yared, Fouad
Mutschler, Angela
Mathieu, Eric
Lavalle, Philippe
Metz-Boutigue, Marie-Hélène
Haïkel, Youssef
The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title_full The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title_fullStr The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title_full_unstemmed The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title_short The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis
title_sort catestatin-derived peptides are new actors to fight the development of oral candidosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876135/
https://www.ncbi.nlm.nih.gov/pubmed/35216181
http://dx.doi.org/10.3390/ijms23042066
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