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Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles
The role of uropathogenic Escherichia coli (UPEC) in colonization and infection of female patients with anatomical and functional abnormalities of the urinary system is elusive. In this study, the phenotype, genotype and the phylogeny of UPEC strains isolated from the urine of pediatric female patie...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876236/ https://www.ncbi.nlm.nih.gov/pubmed/35215173 http://dx.doi.org/10.3390/pathogens11020231 |
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author | Domingos, Marta de Oliveira da Silva Junior, Silvio Marciano Milanello, Wagner Nakano, Shirley Sizue Nakamura Franzolin, Marcia Regina dos Santos, Luis Fernando Nunes, Kamila Oliveira Marques, Vaniky Duarte Elias, Waldir P. Silva, Herbert Guimarães de Sousa Caetano, Bruna De Lucca Piazza, Roxane Maria Fontes |
author_facet | Domingos, Marta de Oliveira da Silva Junior, Silvio Marciano Milanello, Wagner Nakano, Shirley Sizue Nakamura Franzolin, Marcia Regina dos Santos, Luis Fernando Nunes, Kamila Oliveira Marques, Vaniky Duarte Elias, Waldir P. Silva, Herbert Guimarães de Sousa Caetano, Bruna De Lucca Piazza, Roxane Maria Fontes |
author_sort | Domingos, Marta de Oliveira |
collection | PubMed |
description | The role of uropathogenic Escherichia coli (UPEC) in colonization and infection of female patients with anatomical and functional abnormalities of the urinary system is elusive. In this study, the phenotype, genotype and the phylogeny of UPEC strains isolated from the urine of pediatric female patients with cystitis of normal and abnormal urinary tract were determined. Multiplex PCR results demonstrated that 86% of the strains isolated from female patients with normal urinary tract (NUT), belonged to the phylo-groups B2 and D. Their prevalence decreased to 23% in strains isolated from patients with abnormal urinary tract (AUT). More of the isolates from AUT patients produced a biofilm on polystyrene and polyvinyl chloride (PVC), adhered to epithelial cells, and encoded pap and sfa genes than strains isolated from female patients with NUT. In contrast, a higher number of hemolysin-producing strains with serogroups associated with UPEC were isolated from patients with NUT. In summary, the results suggest that cystitis in female patients with NUT is associated with ExPEC, whereas cystitis in female patients with AUT is associated with pathogenic intestinal E. coli strains that have acquired the ability to colonize the bladder. |
format | Online Article Text |
id | pubmed-8876236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88762362022-02-26 Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles Domingos, Marta de Oliveira da Silva Junior, Silvio Marciano Milanello, Wagner Nakano, Shirley Sizue Nakamura Franzolin, Marcia Regina dos Santos, Luis Fernando Nunes, Kamila Oliveira Marques, Vaniky Duarte Elias, Waldir P. Silva, Herbert Guimarães de Sousa Caetano, Bruna De Lucca Piazza, Roxane Maria Fontes Pathogens Article The role of uropathogenic Escherichia coli (UPEC) in colonization and infection of female patients with anatomical and functional abnormalities of the urinary system is elusive. In this study, the phenotype, genotype and the phylogeny of UPEC strains isolated from the urine of pediatric female patients with cystitis of normal and abnormal urinary tract were determined. Multiplex PCR results demonstrated that 86% of the strains isolated from female patients with normal urinary tract (NUT), belonged to the phylo-groups B2 and D. Their prevalence decreased to 23% in strains isolated from patients with abnormal urinary tract (AUT). More of the isolates from AUT patients produced a biofilm on polystyrene and polyvinyl chloride (PVC), adhered to epithelial cells, and encoded pap and sfa genes than strains isolated from female patients with NUT. In contrast, a higher number of hemolysin-producing strains with serogroups associated with UPEC were isolated from patients with NUT. In summary, the results suggest that cystitis in female patients with NUT is associated with ExPEC, whereas cystitis in female patients with AUT is associated with pathogenic intestinal E. coli strains that have acquired the ability to colonize the bladder. MDPI 2022-02-10 /pmc/articles/PMC8876236/ /pubmed/35215173 http://dx.doi.org/10.3390/pathogens11020231 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Domingos, Marta de Oliveira da Silva Junior, Silvio Marciano Milanello, Wagner Nakano, Shirley Sizue Nakamura Franzolin, Marcia Regina dos Santos, Luis Fernando Nunes, Kamila Oliveira Marques, Vaniky Duarte Elias, Waldir P. Silva, Herbert Guimarães de Sousa Caetano, Bruna De Lucca Piazza, Roxane Maria Fontes Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title | Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title_full | Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title_fullStr | Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title_full_unstemmed | Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title_short | Escherichia coli Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles |
title_sort | escherichia coli strains responsible for cystitis in female pediatric patients with normal and abnormal urinary tracts have different virulence profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876236/ https://www.ncbi.nlm.nih.gov/pubmed/35215173 http://dx.doi.org/10.3390/pathogens11020231 |
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