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Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses

African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β pola...

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Autores principales: Franzoni, Giulia, Zinellu, Susanna, Carta, Tania, De Ciucis, Chiara Grazia, Fruscione, Floriana, Anfossi, Antonio, Ledda, Mauro, Graham, Simon P., Dei Giudici, Silvia, Razzuoli, Elisabetta, Oggiano, Annalisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876267/
https://www.ncbi.nlm.nih.gov/pubmed/35215110
http://dx.doi.org/10.3390/pathogens11020166
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author Franzoni, Giulia
Zinellu, Susanna
Carta, Tania
De Ciucis, Chiara Grazia
Fruscione, Floriana
Anfossi, Antonio
Ledda, Mauro
Graham, Simon P.
Dei Giudici, Silvia
Razzuoli, Elisabetta
Oggiano, Annalisa
author_facet Franzoni, Giulia
Zinellu, Susanna
Carta, Tania
De Ciucis, Chiara Grazia
Fruscione, Floriana
Anfossi, Antonio
Ledda, Mauro
Graham, Simon P.
Dei Giudici, Silvia
Razzuoli, Elisabetta
Oggiano, Annalisa
author_sort Franzoni, Giulia
collection PubMed
description African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β polarize macrophages toward an anti-inflammatory phenotype, we analyzed their impact on porcine monocyte-derived macrophages’ (moMΦ) susceptibility to infection and their responses to two genotype I ASFV strains, virulent 26544/OG10 and attenuated NH/P68. At a low multiplicity of infection (MOI), NH/P68, but not 26544/OG10, presented a higher ability to infect moM(IL-10) compared to moMΦ and moM(TGF-β), but no differences were appreciated at a higher MOI. Both strains replicated efficiently in all moMΦ subsets, with no differences at later times post-infection. Both strains downregulated CD14 and CD16 expression on moMΦ, irrespective of the activation status. ASFV’s modulation of CD163 and MHC II DR expression and cytokine responses to NH/P68 or 26544/OG10 ASFV were not affected by either IL-10 or TGF-β pre-treatment. Our results revealed little impact of these anti-inflammatory cytokines on moMΦ interaction with ASFV, which likely reflects the ability of the virus to effectively modulate macrophage responses.
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spelling pubmed-88762672022-02-26 Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses Franzoni, Giulia Zinellu, Susanna Carta, Tania De Ciucis, Chiara Grazia Fruscione, Floriana Anfossi, Antonio Ledda, Mauro Graham, Simon P. Dei Giudici, Silvia Razzuoli, Elisabetta Oggiano, Annalisa Pathogens Article African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β polarize macrophages toward an anti-inflammatory phenotype, we analyzed their impact on porcine monocyte-derived macrophages’ (moMΦ) susceptibility to infection and their responses to two genotype I ASFV strains, virulent 26544/OG10 and attenuated NH/P68. At a low multiplicity of infection (MOI), NH/P68, but not 26544/OG10, presented a higher ability to infect moM(IL-10) compared to moMΦ and moM(TGF-β), but no differences were appreciated at a higher MOI. Both strains replicated efficiently in all moMΦ subsets, with no differences at later times post-infection. Both strains downregulated CD14 and CD16 expression on moMΦ, irrespective of the activation status. ASFV’s modulation of CD163 and MHC II DR expression and cytokine responses to NH/P68 or 26544/OG10 ASFV were not affected by either IL-10 or TGF-β pre-treatment. Our results revealed little impact of these anti-inflammatory cytokines on moMΦ interaction with ASFV, which likely reflects the ability of the virus to effectively modulate macrophage responses. MDPI 2022-01-27 /pmc/articles/PMC8876267/ /pubmed/35215110 http://dx.doi.org/10.3390/pathogens11020166 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Franzoni, Giulia
Zinellu, Susanna
Carta, Tania
De Ciucis, Chiara Grazia
Fruscione, Floriana
Anfossi, Antonio
Ledda, Mauro
Graham, Simon P.
Dei Giudici, Silvia
Razzuoli, Elisabetta
Oggiano, Annalisa
Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title_full Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title_fullStr Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title_full_unstemmed Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title_short Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
title_sort analyses of the impact of immunosuppressive cytokines on porcine macrophage responses and susceptibility to infection to african swine fever viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876267/
https://www.ncbi.nlm.nih.gov/pubmed/35215110
http://dx.doi.org/10.3390/pathogens11020166
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