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Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses
African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β pola...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876267/ https://www.ncbi.nlm.nih.gov/pubmed/35215110 http://dx.doi.org/10.3390/pathogens11020166 |
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author | Franzoni, Giulia Zinellu, Susanna Carta, Tania De Ciucis, Chiara Grazia Fruscione, Floriana Anfossi, Antonio Ledda, Mauro Graham, Simon P. Dei Giudici, Silvia Razzuoli, Elisabetta Oggiano, Annalisa |
author_facet | Franzoni, Giulia Zinellu, Susanna Carta, Tania De Ciucis, Chiara Grazia Fruscione, Floriana Anfossi, Antonio Ledda, Mauro Graham, Simon P. Dei Giudici, Silvia Razzuoli, Elisabetta Oggiano, Annalisa |
author_sort | Franzoni, Giulia |
collection | PubMed |
description | African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β polarize macrophages toward an anti-inflammatory phenotype, we analyzed their impact on porcine monocyte-derived macrophages’ (moMΦ) susceptibility to infection and their responses to two genotype I ASFV strains, virulent 26544/OG10 and attenuated NH/P68. At a low multiplicity of infection (MOI), NH/P68, but not 26544/OG10, presented a higher ability to infect moM(IL-10) compared to moMΦ and moM(TGF-β), but no differences were appreciated at a higher MOI. Both strains replicated efficiently in all moMΦ subsets, with no differences at later times post-infection. Both strains downregulated CD14 and CD16 expression on moMΦ, irrespective of the activation status. ASFV’s modulation of CD163 and MHC II DR expression and cytokine responses to NH/P68 or 26544/OG10 ASFV were not affected by either IL-10 or TGF-β pre-treatment. Our results revealed little impact of these anti-inflammatory cytokines on moMΦ interaction with ASFV, which likely reflects the ability of the virus to effectively modulate macrophage responses. |
format | Online Article Text |
id | pubmed-8876267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88762672022-02-26 Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses Franzoni, Giulia Zinellu, Susanna Carta, Tania De Ciucis, Chiara Grazia Fruscione, Floriana Anfossi, Antonio Ledda, Mauro Graham, Simon P. Dei Giudici, Silvia Razzuoli, Elisabetta Oggiano, Annalisa Pathogens Article African swine fever viruses (ASFV), currently a serious threat to the global pig industry, primarily target porcine macrophages. Macrophages are characterized by their remarkable plasticity, being able to modify their phenotype and functions in response to diverse stimuli. Since IL-10 and TGF-β polarize macrophages toward an anti-inflammatory phenotype, we analyzed their impact on porcine monocyte-derived macrophages’ (moMΦ) susceptibility to infection and their responses to two genotype I ASFV strains, virulent 26544/OG10 and attenuated NH/P68. At a low multiplicity of infection (MOI), NH/P68, but not 26544/OG10, presented a higher ability to infect moM(IL-10) compared to moMΦ and moM(TGF-β), but no differences were appreciated at a higher MOI. Both strains replicated efficiently in all moMΦ subsets, with no differences at later times post-infection. Both strains downregulated CD14 and CD16 expression on moMΦ, irrespective of the activation status. ASFV’s modulation of CD163 and MHC II DR expression and cytokine responses to NH/P68 or 26544/OG10 ASFV were not affected by either IL-10 or TGF-β pre-treatment. Our results revealed little impact of these anti-inflammatory cytokines on moMΦ interaction with ASFV, which likely reflects the ability of the virus to effectively modulate macrophage responses. MDPI 2022-01-27 /pmc/articles/PMC8876267/ /pubmed/35215110 http://dx.doi.org/10.3390/pathogens11020166 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Franzoni, Giulia Zinellu, Susanna Carta, Tania De Ciucis, Chiara Grazia Fruscione, Floriana Anfossi, Antonio Ledda, Mauro Graham, Simon P. Dei Giudici, Silvia Razzuoli, Elisabetta Oggiano, Annalisa Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title | Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title_full | Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title_fullStr | Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title_full_unstemmed | Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title_short | Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses |
title_sort | analyses of the impact of immunosuppressive cytokines on porcine macrophage responses and susceptibility to infection to african swine fever viruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876267/ https://www.ncbi.nlm.nih.gov/pubmed/35215110 http://dx.doi.org/10.3390/pathogens11020166 |
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