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Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and sp...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876746/ https://www.ncbi.nlm.nih.gov/pubmed/35213985 http://dx.doi.org/10.3390/pharmaceutics14020252 |
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author | Pisani, Silvia Croce, Stefania Mauramati, Simone Marmonti, Marta Cobianchi, Lorenzo Herman, Irene Dorati, Rossella Avanzini, Maria Antonietta Genta, Ida Benazzo, Marco Conti, Bice |
author_facet | Pisani, Silvia Croce, Stefania Mauramati, Simone Marmonti, Marta Cobianchi, Lorenzo Herman, Irene Dorati, Rossella Avanzini, Maria Antonietta Genta, Ida Benazzo, Marco Conti, Bice |
author_sort | Pisani, Silvia |
collection | PubMed |
description | Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture. |
format | Online Article Text |
id | pubmed-8876746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88767462022-02-26 Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach Pisani, Silvia Croce, Stefania Mauramati, Simone Marmonti, Marta Cobianchi, Lorenzo Herman, Irene Dorati, Rossella Avanzini, Maria Antonietta Genta, Ida Benazzo, Marco Conti, Bice Pharmaceutics Article Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture. MDPI 2022-01-21 /pmc/articles/PMC8876746/ /pubmed/35213985 http://dx.doi.org/10.3390/pharmaceutics14020252 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pisani, Silvia Croce, Stefania Mauramati, Simone Marmonti, Marta Cobianchi, Lorenzo Herman, Irene Dorati, Rossella Avanzini, Maria Antonietta Genta, Ida Benazzo, Marco Conti, Bice Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title_full | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title_fullStr | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title_full_unstemmed | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title_short | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
title_sort | engineered full thickness electrospun scaffold for esophageal tissue regeneration: from in vitro to in vivo approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876746/ https://www.ncbi.nlm.nih.gov/pubmed/35213985 http://dx.doi.org/10.3390/pharmaceutics14020252 |
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