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Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach

Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and sp...

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Autores principales: Pisani, Silvia, Croce, Stefania, Mauramati, Simone, Marmonti, Marta, Cobianchi, Lorenzo, Herman, Irene, Dorati, Rossella, Avanzini, Maria Antonietta, Genta, Ida, Benazzo, Marco, Conti, Bice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876746/
https://www.ncbi.nlm.nih.gov/pubmed/35213985
http://dx.doi.org/10.3390/pharmaceutics14020252
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author Pisani, Silvia
Croce, Stefania
Mauramati, Simone
Marmonti, Marta
Cobianchi, Lorenzo
Herman, Irene
Dorati, Rossella
Avanzini, Maria Antonietta
Genta, Ida
Benazzo, Marco
Conti, Bice
author_facet Pisani, Silvia
Croce, Stefania
Mauramati, Simone
Marmonti, Marta
Cobianchi, Lorenzo
Herman, Irene
Dorati, Rossella
Avanzini, Maria Antonietta
Genta, Ida
Benazzo, Marco
Conti, Bice
author_sort Pisani, Silvia
collection PubMed
description Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture.
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spelling pubmed-88767462022-02-26 Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach Pisani, Silvia Croce, Stefania Mauramati, Simone Marmonti, Marta Cobianchi, Lorenzo Herman, Irene Dorati, Rossella Avanzini, Maria Antonietta Genta, Ida Benazzo, Marco Conti, Bice Pharmaceutics Article Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture. MDPI 2022-01-21 /pmc/articles/PMC8876746/ /pubmed/35213985 http://dx.doi.org/10.3390/pharmaceutics14020252 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pisani, Silvia
Croce, Stefania
Mauramati, Simone
Marmonti, Marta
Cobianchi, Lorenzo
Herman, Irene
Dorati, Rossella
Avanzini, Maria Antonietta
Genta, Ida
Benazzo, Marco
Conti, Bice
Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title_full Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title_fullStr Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title_full_unstemmed Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title_short Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
title_sort engineered full thickness electrospun scaffold for esophageal tissue regeneration: from in vitro to in vivo approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876746/
https://www.ncbi.nlm.nih.gov/pubmed/35213985
http://dx.doi.org/10.3390/pharmaceutics14020252
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