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Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein

Aedes aegypti mosquitoes are important vectors of several debilitating and deadly arthropod-borne (arbo) viruses, including Yellow Fever virus, Dengue virus, West Nile virus and Zika virus (ZIKV). Arbovirus transmission occurs when an infected mosquito probes the host’s skin in search of a blood mea...

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Autores principales: Valenzuela-Leon, Paola Carolina, Shrivastava, Gaurav, Martin-Martin, Ines, Cardenas, Jenny C., Londono-Renteria, Berlin, Calvo, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876891/
https://www.ncbi.nlm.nih.gov/pubmed/35215815
http://dx.doi.org/10.3390/v14020221
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author Valenzuela-Leon, Paola Carolina
Shrivastava, Gaurav
Martin-Martin, Ines
Cardenas, Jenny C.
Londono-Renteria, Berlin
Calvo, Eric
author_facet Valenzuela-Leon, Paola Carolina
Shrivastava, Gaurav
Martin-Martin, Ines
Cardenas, Jenny C.
Londono-Renteria, Berlin
Calvo, Eric
author_sort Valenzuela-Leon, Paola Carolina
collection PubMed
description Aedes aegypti mosquitoes are important vectors of several debilitating and deadly arthropod-borne (arbo) viruses, including Yellow Fever virus, Dengue virus, West Nile virus and Zika virus (ZIKV). Arbovirus transmission occurs when an infected mosquito probes the host’s skin in search of a blood meal. Salivary proteins from mosquitoes help to acquire blood and have also been shown to enhance pathogen transmission in vivo and in vitro. Here, we evaluated the interaction of mosquito salivary proteins with ZIKV by surface plasmon resonance and enzyme-linked immunosorbent assay. We found that three salivary proteins AAEL000793, AAEL007420, and AAEL006347 bind to the envelope protein of ZIKV with nanomolar affinities. Similar results were obtained using virus-like particles in binding assays. These interactions have no effect on viral replication in cultured endothelial cells and keratinocytes. Additionally, we found detectable antibody levels in ZIKV and DENV serum samples against the recombinant proteins that interact with ZIKV. These results highlight complex interactions between viruses, salivary proteins and antibodies that could be present during viral transmissions.
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spelling pubmed-88768912022-02-26 Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein Valenzuela-Leon, Paola Carolina Shrivastava, Gaurav Martin-Martin, Ines Cardenas, Jenny C. Londono-Renteria, Berlin Calvo, Eric Viruses Article Aedes aegypti mosquitoes are important vectors of several debilitating and deadly arthropod-borne (arbo) viruses, including Yellow Fever virus, Dengue virus, West Nile virus and Zika virus (ZIKV). Arbovirus transmission occurs when an infected mosquito probes the host’s skin in search of a blood meal. Salivary proteins from mosquitoes help to acquire blood and have also been shown to enhance pathogen transmission in vivo and in vitro. Here, we evaluated the interaction of mosquito salivary proteins with ZIKV by surface plasmon resonance and enzyme-linked immunosorbent assay. We found that three salivary proteins AAEL000793, AAEL007420, and AAEL006347 bind to the envelope protein of ZIKV with nanomolar affinities. Similar results were obtained using virus-like particles in binding assays. These interactions have no effect on viral replication in cultured endothelial cells and keratinocytes. Additionally, we found detectable antibody levels in ZIKV and DENV serum samples against the recombinant proteins that interact with ZIKV. These results highlight complex interactions between viruses, salivary proteins and antibodies that could be present during viral transmissions. MDPI 2022-01-24 /pmc/articles/PMC8876891/ /pubmed/35215815 http://dx.doi.org/10.3390/v14020221 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Valenzuela-Leon, Paola Carolina
Shrivastava, Gaurav
Martin-Martin, Ines
Cardenas, Jenny C.
Londono-Renteria, Berlin
Calvo, Eric
Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title_full Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title_fullStr Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title_full_unstemmed Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title_short Multiple Salivary Proteins from Aedes aegypti Mosquito Bind to the Zika Virus Envelope Protein
title_sort multiple salivary proteins from aedes aegypti mosquito bind to the zika virus envelope protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876891/
https://www.ncbi.nlm.nih.gov/pubmed/35215815
http://dx.doi.org/10.3390/v14020221
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