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Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides

Galacto-oligosaccharides (GOS) are prebiotic sugars obtained enzymatically from lactose and used in food industry due to their nutritional advantages or technological properties. Selective mass transport and enzymatic synthesis were integrated and followed using a membrane bioreactor, so that select...

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Autores principales: Botelho, Vanessa A., Mateus, Marília, Petrus, José C. C., de Pinho, Maria Norberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876974/
https://www.ncbi.nlm.nih.gov/pubmed/35207092
http://dx.doi.org/10.3390/membranes12020171
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author Botelho, Vanessa A.
Mateus, Marília
Petrus, José C. C.
de Pinho, Maria Norberta
author_facet Botelho, Vanessa A.
Mateus, Marília
Petrus, José C. C.
de Pinho, Maria Norberta
author_sort Botelho, Vanessa A.
collection PubMed
description Galacto-oligosaccharides (GOS) are prebiotic sugars obtained enzymatically from lactose and used in food industry due to their nutritional advantages or technological properties. Selective mass transport and enzymatic synthesis were integrated and followed using a membrane bioreactor, so that selective removal of reaction products may lead to increased conversions of product-inhibited or thermodynamically unfavorable reactions. GOS syntheses were conducted on lactose solutions (150 g·L(−1)) at 40 °C and 10 U(β-galactosidase).mL(−1), and sugar fractionation was performed by cellulose acetate membranes. Effects of pressure (20; 24 bar) and crossflow velocity (1.7; 2.0; 2.4 m·s(−1)) on bioreactor performance were studied. Simultaneous GOS synthesis and production fractionation increased GOS production by 60%, in comparison to the same reactions promoted without permeation. The presence of a high-molecular-weight solute, the enzyme, in association with high total sugar concentration, leads to complex selective mass transfer characteristics. Without the enzyme, the membrane presented tight ultrafiltration characteristics, permeating mono- and disaccharides and retaining just 25% of trisaccharides. During simultaneous synthesis and fractionation, GOS-3 were totally retained, and GOS-2 and monosaccharides were retained at 80% and 40%, respectively. GOS synthesis—hydrolysis evolution was strongly dependent on crossflow velocity at 20 bar but became fairly independent at 24 bar.
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spelling pubmed-88769742022-02-26 Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides Botelho, Vanessa A. Mateus, Marília Petrus, José C. C. de Pinho, Maria Norberta Membranes (Basel) Article Galacto-oligosaccharides (GOS) are prebiotic sugars obtained enzymatically from lactose and used in food industry due to their nutritional advantages or technological properties. Selective mass transport and enzymatic synthesis were integrated and followed using a membrane bioreactor, so that selective removal of reaction products may lead to increased conversions of product-inhibited or thermodynamically unfavorable reactions. GOS syntheses were conducted on lactose solutions (150 g·L(−1)) at 40 °C and 10 U(β-galactosidase).mL(−1), and sugar fractionation was performed by cellulose acetate membranes. Effects of pressure (20; 24 bar) and crossflow velocity (1.7; 2.0; 2.4 m·s(−1)) on bioreactor performance were studied. Simultaneous GOS synthesis and production fractionation increased GOS production by 60%, in comparison to the same reactions promoted without permeation. The presence of a high-molecular-weight solute, the enzyme, in association with high total sugar concentration, leads to complex selective mass transfer characteristics. Without the enzyme, the membrane presented tight ultrafiltration characteristics, permeating mono- and disaccharides and retaining just 25% of trisaccharides. During simultaneous synthesis and fractionation, GOS-3 were totally retained, and GOS-2 and monosaccharides were retained at 80% and 40%, respectively. GOS synthesis—hydrolysis evolution was strongly dependent on crossflow velocity at 20 bar but became fairly independent at 24 bar. MDPI 2022-01-31 /pmc/articles/PMC8876974/ /pubmed/35207092 http://dx.doi.org/10.3390/membranes12020171 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Botelho, Vanessa A.
Mateus, Marília
Petrus, José C. C.
de Pinho, Maria Norberta
Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title_full Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title_fullStr Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title_full_unstemmed Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title_short Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides
title_sort membrane bioreactor for simultaneous synthesis and fractionation of oligosaccharides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876974/
https://www.ncbi.nlm.nih.gov/pubmed/35207092
http://dx.doi.org/10.3390/membranes12020171
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