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Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears

Loss of active synapses and alterations in membrane lipids are crucial events in physiological aging as well as in neurodegenerative disorders. Both are related to the abnormal aggregation of amyloid-beta (Aβ) species, generally known as amyloidosis. There are two major known human Aβ species: Aβ((1...

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Autores principales: Van Bulck, Michiel, Brandt, Nicola, Claus, Ralf A., Gräler, Markus, Bräuer, Anja U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877175/
https://www.ncbi.nlm.nih.gov/pubmed/35216416
http://dx.doi.org/10.3390/ijms23042300
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author Van Bulck, Michiel
Brandt, Nicola
Claus, Ralf A.
Gräler, Markus
Bräuer, Anja U.
author_facet Van Bulck, Michiel
Brandt, Nicola
Claus, Ralf A.
Gräler, Markus
Bräuer, Anja U.
author_sort Van Bulck, Michiel
collection PubMed
description Loss of active synapses and alterations in membrane lipids are crucial events in physiological aging as well as in neurodegenerative disorders. Both are related to the abnormal aggregation of amyloid-beta (Aβ) species, generally known as amyloidosis. There are two major known human Aβ species: Aβ((1–40)) and Aβ((1–42)). However, which of these species have more influence on active synapses and membrane lipids is still poorly understood. Additionally, the time-dependent effect of Aβ species on alterations in membrane lipids of hippocampal neurones and glial cells remains unknown. Therefore, our study contributes to a better understanding of the role of Aβ species in the loss of active synapses and the dysregulation of membrane lipids in vitro. We showed that Aβ((1–40)) or Aβ((1–42)) treatment influences membrane lipids before synaptic loss appears and that the loss of active synapses is not dependent on the Aβ species. Our lipidomic data analysis showed early changes in specific lipid classes such as sphingolipid and glycerophospholipid neurones. Our results underscore the potential role of lipids as a possible early diagnostic biomarker in amyloidosis-related disorders.
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spelling pubmed-88771752022-02-26 Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears Van Bulck, Michiel Brandt, Nicola Claus, Ralf A. Gräler, Markus Bräuer, Anja U. Int J Mol Sci Article Loss of active synapses and alterations in membrane lipids are crucial events in physiological aging as well as in neurodegenerative disorders. Both are related to the abnormal aggregation of amyloid-beta (Aβ) species, generally known as amyloidosis. There are two major known human Aβ species: Aβ((1–40)) and Aβ((1–42)). However, which of these species have more influence on active synapses and membrane lipids is still poorly understood. Additionally, the time-dependent effect of Aβ species on alterations in membrane lipids of hippocampal neurones and glial cells remains unknown. Therefore, our study contributes to a better understanding of the role of Aβ species in the loss of active synapses and the dysregulation of membrane lipids in vitro. We showed that Aβ((1–40)) or Aβ((1–42)) treatment influences membrane lipids before synaptic loss appears and that the loss of active synapses is not dependent on the Aβ species. Our lipidomic data analysis showed early changes in specific lipid classes such as sphingolipid and glycerophospholipid neurones. Our results underscore the potential role of lipids as a possible early diagnostic biomarker in amyloidosis-related disorders. MDPI 2022-02-19 /pmc/articles/PMC8877175/ /pubmed/35216416 http://dx.doi.org/10.3390/ijms23042300 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Van Bulck, Michiel
Brandt, Nicola
Claus, Ralf A.
Gräler, Markus
Bräuer, Anja U.
Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title_full Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title_fullStr Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title_full_unstemmed Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title_short Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears
title_sort aβ-induced alterations in membrane lipids occur before synaptic loss appears
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877175/
https://www.ncbi.nlm.nih.gov/pubmed/35216416
http://dx.doi.org/10.3390/ijms23042300
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