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Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus

Porcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epid...

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Autores principales: Ibrahim, Yassein M., Zhang, Wenli, Werid, Gebremeskel Mamu, Zhang, He, Feng, Yawen, Pan, Yu, Zhang, Lin, Li, Changwen, Lin, Huan, Chen, Hongyan, Wang, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877214/
https://www.ncbi.nlm.nih.gov/pubmed/35215935
http://dx.doi.org/10.3390/v14020349
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author Ibrahim, Yassein M.
Zhang, Wenli
Werid, Gebremeskel Mamu
Zhang, He
Feng, Yawen
Pan, Yu
Zhang, Lin
Li, Changwen
Lin, Huan
Chen, Hongyan
Wang, Yue
author_facet Ibrahim, Yassein M.
Zhang, Wenli
Werid, Gebremeskel Mamu
Zhang, He
Feng, Yawen
Pan, Yu
Zhang, Lin
Li, Changwen
Lin, Huan
Chen, Hongyan
Wang, Yue
author_sort Ibrahim, Yassein M.
collection PubMed
description Porcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epidemiological studies are not yet available. Here, four PSV strains were recovered from diarrheic piglets, and electron microscopy revealed virus particles with a diameter of ~32 nm. Analysis of the entire genome sequence revealed that the genomes of PSV isolates ranged 7569–7572 nucleotides in length. Phylogenetic analysis showed that the isolated viruses were classified together with strains from China. Additionally, monoclonal antibodies for the recombinant PSV-VP1 protein were developed to specifically detect PSV infection in cells, and we demonstrated that isolated PSVs could only replicate in cells of porcine origin. Using recombinant PSV-VP1 protein as the coating antigen, we developed an indirect ELISA for the first time for the detection of PSV antibodies in serum. A total of 516 swine serum samples were tested, and PSV positive rate was 79.3%. The virus isolates, monoclonal antibodies and indirect ELISA developed would be useful for further understanding the pathophysiology of PSV, developing new diagnostic assays, and investigating the epidemiology of the PSV.
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spelling pubmed-88772142022-02-26 Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus Ibrahim, Yassein M. Zhang, Wenli Werid, Gebremeskel Mamu Zhang, He Feng, Yawen Pan, Yu Zhang, Lin Li, Changwen Lin, Huan Chen, Hongyan Wang, Yue Viruses Article Porcine sapelovirus (PSV) is an important emerging pathogen associated with a wide variety of diseases in swine, including acute diarrhoea, respiratory distress, skin lesions, severe neurological disorders, and reproductive failure. Although PSV is widespread, serological assays for field-based epidemiological studies are not yet available. Here, four PSV strains were recovered from diarrheic piglets, and electron microscopy revealed virus particles with a diameter of ~32 nm. Analysis of the entire genome sequence revealed that the genomes of PSV isolates ranged 7569–7572 nucleotides in length. Phylogenetic analysis showed that the isolated viruses were classified together with strains from China. Additionally, monoclonal antibodies for the recombinant PSV-VP1 protein were developed to specifically detect PSV infection in cells, and we demonstrated that isolated PSVs could only replicate in cells of porcine origin. Using recombinant PSV-VP1 protein as the coating antigen, we developed an indirect ELISA for the first time for the detection of PSV antibodies in serum. A total of 516 swine serum samples were tested, and PSV positive rate was 79.3%. The virus isolates, monoclonal antibodies and indirect ELISA developed would be useful for further understanding the pathophysiology of PSV, developing new diagnostic assays, and investigating the epidemiology of the PSV. MDPI 2022-02-08 /pmc/articles/PMC8877214/ /pubmed/35215935 http://dx.doi.org/10.3390/v14020349 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ibrahim, Yassein M.
Zhang, Wenli
Werid, Gebremeskel Mamu
Zhang, He
Feng, Yawen
Pan, Yu
Zhang, Lin
Li, Changwen
Lin, Huan
Chen, Hongyan
Wang, Yue
Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title_full Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title_fullStr Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title_full_unstemmed Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title_short Isolation, Characterization, and Molecular Detection of Porcine Sapelovirus
title_sort isolation, characterization, and molecular detection of porcine sapelovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877214/
https://www.ncbi.nlm.nih.gov/pubmed/35215935
http://dx.doi.org/10.3390/v14020349
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