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Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage

The simultaneous cytological and metabolic investigation of various extravascular body fluids (EBFs) provides clinically relevant information about the type and intensity of the immune response in particular organ systems. The oxidative burst of professional phagocytes with the concomitant productio...

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Autores principales: Kelbich, Petr, Vachata, Petr, Maly, Vilem, Novotny, Tomas, Spicka, Jan, Matuchova, Inka, Radovnicky, Tomas, Stanek, Ivan, Kubalik, Jan, Karpjuk, Ondrej, Smisko, Frantisek, Hanuljakova, Eva, Krejsek, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877237/
https://www.ncbi.nlm.nih.gov/pubmed/35207447
http://dx.doi.org/10.3390/life12020160
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author Kelbich, Petr
Vachata, Petr
Maly, Vilem
Novotny, Tomas
Spicka, Jan
Matuchova, Inka
Radovnicky, Tomas
Stanek, Ivan
Kubalik, Jan
Karpjuk, Ondrej
Smisko, Frantisek
Hanuljakova, Eva
Krejsek, Jan
author_facet Kelbich, Petr
Vachata, Petr
Maly, Vilem
Novotny, Tomas
Spicka, Jan
Matuchova, Inka
Radovnicky, Tomas
Stanek, Ivan
Kubalik, Jan
Karpjuk, Ondrej
Smisko, Frantisek
Hanuljakova, Eva
Krejsek, Jan
author_sort Kelbich, Petr
collection PubMed
description The simultaneous cytological and metabolic investigation of various extravascular body fluids (EBFs) provides clinically relevant information about the type and intensity of the immune response in particular organ systems. The oxidative burst of professional phagocytes with the concomitant production of reactive oxygen species consumes a large amount of oxygen and is the cause of switch to the development of anaerobic metabolism. We assessed the relationships between percentages of neutrophils, aerobic and anaerobic metabolism, and tissue damage via the determination of aspartate aminotransferase catalytic activities (AST) in cerebrospinal fluid (CSF), pleural effusions (PE), abdominal effusions (AE), and synovial fluids (SF). EBFs with 0.0–20.0% neutrophils: 83.0% aerobic and 1.3% strongly anaerobic cases with median of AST = 13.8 IU/L in CSF; 68.0% aerobic and 9.0% strongly anaerobic cases with median of AST = 20.4 IU/L in PE; 77.5% aerobic and 10.5% strongly anaerobic cases with median of AST = 18.0 IU/L in AE; 64.1% aerobic and 7.7% strongly anaerobic cases with median of AST = 13.8 IU/L in SF. EBFs with 80.0–100.0% neutrophils: 4.2% aerobic and 73.7% strongly anaerobic cases with median of AST = 19.2 IU/L in CSF; 7.4% aerobic and 77.3% strongly anaerobic cases with median of AST = 145.2 IU/L in PE; 11.8% aerobic and 73.7% strongly anaerobic cases with median of AST = 61.8 IU/L in AE; 25.5% aerobic and 38.2% strongly anaerobic cases with median of AST = 37.2 IU/L in SF. The significant presence of neutrophils, concomitant strong anaerobic metabolism, and elevated AST in various EBFs are reliable signs of damaging purulent inflammation.
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spelling pubmed-88772372022-02-26 Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage Kelbich, Petr Vachata, Petr Maly, Vilem Novotny, Tomas Spicka, Jan Matuchova, Inka Radovnicky, Tomas Stanek, Ivan Kubalik, Jan Karpjuk, Ondrej Smisko, Frantisek Hanuljakova, Eva Krejsek, Jan Life (Basel) Article The simultaneous cytological and metabolic investigation of various extravascular body fluids (EBFs) provides clinically relevant information about the type and intensity of the immune response in particular organ systems. The oxidative burst of professional phagocytes with the concomitant production of reactive oxygen species consumes a large amount of oxygen and is the cause of switch to the development of anaerobic metabolism. We assessed the relationships between percentages of neutrophils, aerobic and anaerobic metabolism, and tissue damage via the determination of aspartate aminotransferase catalytic activities (AST) in cerebrospinal fluid (CSF), pleural effusions (PE), abdominal effusions (AE), and synovial fluids (SF). EBFs with 0.0–20.0% neutrophils: 83.0% aerobic and 1.3% strongly anaerobic cases with median of AST = 13.8 IU/L in CSF; 68.0% aerobic and 9.0% strongly anaerobic cases with median of AST = 20.4 IU/L in PE; 77.5% aerobic and 10.5% strongly anaerobic cases with median of AST = 18.0 IU/L in AE; 64.1% aerobic and 7.7% strongly anaerobic cases with median of AST = 13.8 IU/L in SF. EBFs with 80.0–100.0% neutrophils: 4.2% aerobic and 73.7% strongly anaerobic cases with median of AST = 19.2 IU/L in CSF; 7.4% aerobic and 77.3% strongly anaerobic cases with median of AST = 145.2 IU/L in PE; 11.8% aerobic and 73.7% strongly anaerobic cases with median of AST = 61.8 IU/L in AE; 25.5% aerobic and 38.2% strongly anaerobic cases with median of AST = 37.2 IU/L in SF. The significant presence of neutrophils, concomitant strong anaerobic metabolism, and elevated AST in various EBFs are reliable signs of damaging purulent inflammation. MDPI 2022-01-21 /pmc/articles/PMC8877237/ /pubmed/35207447 http://dx.doi.org/10.3390/life12020160 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kelbich, Petr
Vachata, Petr
Maly, Vilem
Novotny, Tomas
Spicka, Jan
Matuchova, Inka
Radovnicky, Tomas
Stanek, Ivan
Kubalik, Jan
Karpjuk, Ondrej
Smisko, Frantisek
Hanuljakova, Eva
Krejsek, Jan
Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title_full Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title_fullStr Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title_full_unstemmed Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title_short Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage
title_sort neutrophils in extravascular body fluids: cytological-energy analysis enables rapid, reliable and inexpensive detection of purulent inflammation and tissue damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877237/
https://www.ncbi.nlm.nih.gov/pubmed/35207447
http://dx.doi.org/10.3390/life12020160
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