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Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host

The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the vi...

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Autores principales: Lengyel, Szabina, Rascle, Christine, Poussereau, Nathalie, Bruel, Christophe, Sella, Luca, Choquer, Mathias, Favaron, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877277/
https://www.ncbi.nlm.nih.gov/pubmed/35208900
http://dx.doi.org/10.3390/microorganisms10020444
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author Lengyel, Szabina
Rascle, Christine
Poussereau, Nathalie
Bruel, Christophe
Sella, Luca
Choquer, Mathias
Favaron, Francesco
author_facet Lengyel, Szabina
Rascle, Christine
Poussereau, Nathalie
Bruel, Christophe
Sella, Luca
Choquer, Mathias
Favaron, Francesco
author_sort Lengyel, Szabina
collection PubMed
description The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the virulence of the necrotrophic fungus Botrytis cinerea, two independent deletion mutants of the Bcsnf1 gene were obtained and analyzed. Virulence of the Δsnf1 mutants was reduced by 59% on a host with acidic pH (apple fruit) and up to 89% on hosts with neutral pH (cucumber cotyledon and French bean leaf). In vitro, Δsnf1 mutants grew slower than the wild type strain at both pH 5 and 7, with a reduction of 20–80% in simple sugars, polysaccharides, and lipidic carbon sources, and these defects were amplified at pH 7. A two-fold reduction in secretion of xylanase activities was observed consequently to the Bcsnf1 gene deletion. Moreover, Δsnf1 mutants were altered in their ability to control ambient pH. Finally, Δsnf1 mutants were impaired in asexual sporulation and did not produce macroconidia. These results confirm the importance of BcSnf1 in pathogenicity, nutrition, and conidiation, and suggest a role in pH regulation for this global regulator in filamentous fungi.
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spelling pubmed-88772772022-02-26 Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host Lengyel, Szabina Rascle, Christine Poussereau, Nathalie Bruel, Christophe Sella, Luca Choquer, Mathias Favaron, Francesco Microorganisms Article The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the virulence of the necrotrophic fungus Botrytis cinerea, two independent deletion mutants of the Bcsnf1 gene were obtained and analyzed. Virulence of the Δsnf1 mutants was reduced by 59% on a host with acidic pH (apple fruit) and up to 89% on hosts with neutral pH (cucumber cotyledon and French bean leaf). In vitro, Δsnf1 mutants grew slower than the wild type strain at both pH 5 and 7, with a reduction of 20–80% in simple sugars, polysaccharides, and lipidic carbon sources, and these defects were amplified at pH 7. A two-fold reduction in secretion of xylanase activities was observed consequently to the Bcsnf1 gene deletion. Moreover, Δsnf1 mutants were altered in their ability to control ambient pH. Finally, Δsnf1 mutants were impaired in asexual sporulation and did not produce macroconidia. These results confirm the importance of BcSnf1 in pathogenicity, nutrition, and conidiation, and suggest a role in pH regulation for this global regulator in filamentous fungi. MDPI 2022-02-15 /pmc/articles/PMC8877277/ /pubmed/35208900 http://dx.doi.org/10.3390/microorganisms10020444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lengyel, Szabina
Rascle, Christine
Poussereau, Nathalie
Bruel, Christophe
Sella, Luca
Choquer, Mathias
Favaron, Francesco
Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title_full Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title_fullStr Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title_full_unstemmed Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title_short Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
title_sort snf1 kinase differentially regulates botrytis cinerea pathogenicity according to the plant host
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877277/
https://www.ncbi.nlm.nih.gov/pubmed/35208900
http://dx.doi.org/10.3390/microorganisms10020444
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