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Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host
The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the vi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877277/ https://www.ncbi.nlm.nih.gov/pubmed/35208900 http://dx.doi.org/10.3390/microorganisms10020444 |
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author | Lengyel, Szabina Rascle, Christine Poussereau, Nathalie Bruel, Christophe Sella, Luca Choquer, Mathias Favaron, Francesco |
author_facet | Lengyel, Szabina Rascle, Christine Poussereau, Nathalie Bruel, Christophe Sella, Luca Choquer, Mathias Favaron, Francesco |
author_sort | Lengyel, Szabina |
collection | PubMed |
description | The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the virulence of the necrotrophic fungus Botrytis cinerea, two independent deletion mutants of the Bcsnf1 gene were obtained and analyzed. Virulence of the Δsnf1 mutants was reduced by 59% on a host with acidic pH (apple fruit) and up to 89% on hosts with neutral pH (cucumber cotyledon and French bean leaf). In vitro, Δsnf1 mutants grew slower than the wild type strain at both pH 5 and 7, with a reduction of 20–80% in simple sugars, polysaccharides, and lipidic carbon sources, and these defects were amplified at pH 7. A two-fold reduction in secretion of xylanase activities was observed consequently to the Bcsnf1 gene deletion. Moreover, Δsnf1 mutants were altered in their ability to control ambient pH. Finally, Δsnf1 mutants were impaired in asexual sporulation and did not produce macroconidia. These results confirm the importance of BcSnf1 in pathogenicity, nutrition, and conidiation, and suggest a role in pH regulation for this global regulator in filamentous fungi. |
format | Online Article Text |
id | pubmed-8877277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88772772022-02-26 Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host Lengyel, Szabina Rascle, Christine Poussereau, Nathalie Bruel, Christophe Sella, Luca Choquer, Mathias Favaron, Francesco Microorganisms Article The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the virulence of the necrotrophic fungus Botrytis cinerea, two independent deletion mutants of the Bcsnf1 gene were obtained and analyzed. Virulence of the Δsnf1 mutants was reduced by 59% on a host with acidic pH (apple fruit) and up to 89% on hosts with neutral pH (cucumber cotyledon and French bean leaf). In vitro, Δsnf1 mutants grew slower than the wild type strain at both pH 5 and 7, with a reduction of 20–80% in simple sugars, polysaccharides, and lipidic carbon sources, and these defects were amplified at pH 7. A two-fold reduction in secretion of xylanase activities was observed consequently to the Bcsnf1 gene deletion. Moreover, Δsnf1 mutants were altered in their ability to control ambient pH. Finally, Δsnf1 mutants were impaired in asexual sporulation and did not produce macroconidia. These results confirm the importance of BcSnf1 in pathogenicity, nutrition, and conidiation, and suggest a role in pH regulation for this global regulator in filamentous fungi. MDPI 2022-02-15 /pmc/articles/PMC8877277/ /pubmed/35208900 http://dx.doi.org/10.3390/microorganisms10020444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lengyel, Szabina Rascle, Christine Poussereau, Nathalie Bruel, Christophe Sella, Luca Choquer, Mathias Favaron, Francesco Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title | Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title_full | Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title_fullStr | Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title_full_unstemmed | Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title_short | Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host |
title_sort | snf1 kinase differentially regulates botrytis cinerea pathogenicity according to the plant host |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877277/ https://www.ncbi.nlm.nih.gov/pubmed/35208900 http://dx.doi.org/10.3390/microorganisms10020444 |
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