Cargando…

Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation

Varicocele is a common pathological condition of testis that is related to male fertility problems. A 3-year age Chios ram had an abnormally enlarged scrotal area, was excluded from reproductive duties, and was euthanized with the owners’ permission. The main pathological finding was the presence of...

Descripción completa

Detalles Bibliográficos
Autores principales: Basioura, Athina, Brellou, Georgia D., Tsakmakidis, Ioannis, Vlemmas, Ioannis, Boscos, Constantin M., Giadinis, Nektarios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877391/
https://www.ncbi.nlm.nih.gov/pubmed/35202339
http://dx.doi.org/10.3390/vetsci9020086
_version_ 1784658409739517952
author Basioura, Athina
Brellou, Georgia D.
Tsakmakidis, Ioannis
Vlemmas, Ioannis
Boscos, Constantin M.
Giadinis, Nektarios
author_facet Basioura, Athina
Brellou, Georgia D.
Tsakmakidis, Ioannis
Vlemmas, Ioannis
Boscos, Constantin M.
Giadinis, Nektarios
author_sort Basioura, Athina
collection PubMed
description Varicocele is a common pathological condition of testis that is related to male fertility problems. A 3-year age Chios ram had an abnormally enlarged scrotal area, was excluded from reproductive duties, and was euthanized with the owners’ permission. The main pathological finding was the presence of bilateral multinodular spermatic cord enlargement with laminated vascular thrombi. Histopathological examination revealed commonly mineralized thrombi within the lumen of veins of the pampiniform plexus, inflammation and testicular degeneration. The epididymides were transported to the laboratory and each cauda region was sliced and washed (8 mL water for injection/epididymis), and the epididymal sperm samples were collected. Sperm motility variables (CASA), viability (eosin-nigrosine), morphology (SpermBlue(®)), and DNA integrity (Acridine Orange Test, AOT) were assessed. The total and progressive motility were low in semen samples of both sides (30.00% and 1.00% vs. 42.60% and 2.50% for left and right epididymis, respectively). Low viability values were observed for both sides (26.00% vs. 23.00% for left and right epididymis, respectively), while sperm morphological abnormalities were within normal limits. No sperm with DNA damage were detected. The results of this case report indicate that varicocele is associated with testis dysfunction and degradation of ram semen quality, mainly affecting motility and kinematics.
format Online
Article
Text
id pubmed-8877391
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88773912022-02-26 Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation Basioura, Athina Brellou, Georgia D. Tsakmakidis, Ioannis Vlemmas, Ioannis Boscos, Constantin M. Giadinis, Nektarios Vet Sci Case Report Varicocele is a common pathological condition of testis that is related to male fertility problems. A 3-year age Chios ram had an abnormally enlarged scrotal area, was excluded from reproductive duties, and was euthanized with the owners’ permission. The main pathological finding was the presence of bilateral multinodular spermatic cord enlargement with laminated vascular thrombi. Histopathological examination revealed commonly mineralized thrombi within the lumen of veins of the pampiniform plexus, inflammation and testicular degeneration. The epididymides were transported to the laboratory and each cauda region was sliced and washed (8 mL water for injection/epididymis), and the epididymal sperm samples were collected. Sperm motility variables (CASA), viability (eosin-nigrosine), morphology (SpermBlue(®)), and DNA integrity (Acridine Orange Test, AOT) were assessed. The total and progressive motility were low in semen samples of both sides (30.00% and 1.00% vs. 42.60% and 2.50% for left and right epididymis, respectively). Low viability values were observed for both sides (26.00% vs. 23.00% for left and right epididymis, respectively), while sperm morphological abnormalities were within normal limits. No sperm with DNA damage were detected. The results of this case report indicate that varicocele is associated with testis dysfunction and degradation of ram semen quality, mainly affecting motility and kinematics. MDPI 2022-02-17 /pmc/articles/PMC8877391/ /pubmed/35202339 http://dx.doi.org/10.3390/vetsci9020086 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Basioura, Athina
Brellou, Georgia D.
Tsakmakidis, Ioannis
Vlemmas, Ioannis
Boscos, Constantin M.
Giadinis, Nektarios
Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title_full Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title_fullStr Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title_full_unstemmed Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title_short Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation
title_sort varicocele in an adult ram: histopathological examination and sperm quality evaluation
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877391/
https://www.ncbi.nlm.nih.gov/pubmed/35202339
http://dx.doi.org/10.3390/vetsci9020086
work_keys_str_mv AT basiouraathina varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation
AT brellougeorgiad varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation
AT tsakmakidisioannis varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation
AT vlemmasioannis varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation
AT boscosconstantinm varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation
AT giadinisnektarios varicoceleinanadultramhistopathologicalexaminationandspermqualityevaluation