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Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells
The formation of a protein layer “corona” on the nanoparticle surface upon entry into a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEG...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877401/ https://www.ncbi.nlm.nih.gov/pubmed/35214171 http://dx.doi.org/10.3390/pharmaceutics14020439 |
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author | Meewan, Jitkasem Somani, Sukrut Laskar, Partha Irving, Craig Mullin, Margaret Woods, Stuart Roberts, Craig W. Alzahrani, Abdullah R. Ferro, Valerie A. McGill, Suzanne Weidt, Stefan Burchmore, Richard Dufès, Christine |
author_facet | Meewan, Jitkasem Somani, Sukrut Laskar, Partha Irving, Craig Mullin, Margaret Woods, Stuart Roberts, Craig W. Alzahrani, Abdullah R. Ferro, Valerie A. McGill, Suzanne Weidt, Stefan Burchmore, Richard Dufès, Christine |
author_sort | Meewan, Jitkasem |
collection | PubMed |
description | The formation of a protein layer “corona” on the nanoparticle surface upon entry into a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrate that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy. |
format | Online Article Text |
id | pubmed-8877401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88774012022-02-26 Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells Meewan, Jitkasem Somani, Sukrut Laskar, Partha Irving, Craig Mullin, Margaret Woods, Stuart Roberts, Craig W. Alzahrani, Abdullah R. Ferro, Valerie A. McGill, Suzanne Weidt, Stefan Burchmore, Richard Dufès, Christine Pharmaceutics Article The formation of a protein layer “corona” on the nanoparticle surface upon entry into a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrate that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy. MDPI 2022-02-18 /pmc/articles/PMC8877401/ /pubmed/35214171 http://dx.doi.org/10.3390/pharmaceutics14020439 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meewan, Jitkasem Somani, Sukrut Laskar, Partha Irving, Craig Mullin, Margaret Woods, Stuart Roberts, Craig W. Alzahrani, Abdullah R. Ferro, Valerie A. McGill, Suzanne Weidt, Stefan Burchmore, Richard Dufès, Christine Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title | Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title_full | Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title_fullStr | Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title_full_unstemmed | Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title_short | Limited Impact of the Protein Corona on the Cellular Uptake of PEGylated Zein Micelles by Melanoma Cancer Cells |
title_sort | limited impact of the protein corona on the cellular uptake of pegylated zein micelles by melanoma cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877401/ https://www.ncbi.nlm.nih.gov/pubmed/35214171 http://dx.doi.org/10.3390/pharmaceutics14020439 |
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