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Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties

Classical treatments of shoulder instability are associated with recurrence. To determine whether the modification of the capsule properties may be an alternative procedure, the effect of crosslinking treatment on the structure and mechanical properties of diseased human shoulder capsules was invest...

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Autores principales: Cornette, Pauline, Jaabar, Ilhem Lilia, Dupres, Vincent, Werthel, Jean-David, Berenbaum, Francis, Houard, Xavier, Landoulsi, Jessem, Nourissat, Geoffroy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877509/
https://www.ncbi.nlm.nih.gov/pubmed/35216412
http://dx.doi.org/10.3390/ijms23042297
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author Cornette, Pauline
Jaabar, Ilhem Lilia
Dupres, Vincent
Werthel, Jean-David
Berenbaum, Francis
Houard, Xavier
Landoulsi, Jessem
Nourissat, Geoffroy
author_facet Cornette, Pauline
Jaabar, Ilhem Lilia
Dupres, Vincent
Werthel, Jean-David
Berenbaum, Francis
Houard, Xavier
Landoulsi, Jessem
Nourissat, Geoffroy
author_sort Cornette, Pauline
collection PubMed
description Classical treatments of shoulder instability are associated with recurrence. To determine whether the modification of the capsule properties may be an alternative procedure, the effect of crosslinking treatment on the structure and mechanical properties of diseased human shoulder capsules was investigated. Joint capsules harvested from patients during shoulder surgery (n = 5) were treated or not with UV and/or riboflavin (0.1%, 1.0% and 2.5%). The structure and the mechanical properties of the capsules were determined by atomic force microscopy. The effect of treatments on cell death was investigated. Collagen fibrils were well-aligned and adjacent to each other with a D-periodicity of 66.9 ± 3.2 nm and a diameter of 71.8 ± 15.4 nm in control untreated capsules. No effect of treatments was observed on the organization of the collagen fibrils nor on their intrinsic characteristics, including D-periodicity or their mean diameter. The treatments also did not induce cell death. In contrast, UV + 2.5% riboflavin induced capsule stiffness, as revealed by the increased Young’s modulus values (p < 0.0001 for each patient). Our results showed that the crosslinking procedure changed the biomechanics of diseased capsules, while keeping their structural organisation unchanged at the single fibril level. The UV/riboflavin crosslinking procedure may be a promising way to preserve the functions of collagen-based tissues and tune their elasticity for clinically relevant treatments.
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spelling pubmed-88775092022-02-26 Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties Cornette, Pauline Jaabar, Ilhem Lilia Dupres, Vincent Werthel, Jean-David Berenbaum, Francis Houard, Xavier Landoulsi, Jessem Nourissat, Geoffroy Int J Mol Sci Article Classical treatments of shoulder instability are associated with recurrence. To determine whether the modification of the capsule properties may be an alternative procedure, the effect of crosslinking treatment on the structure and mechanical properties of diseased human shoulder capsules was investigated. Joint capsules harvested from patients during shoulder surgery (n = 5) were treated or not with UV and/or riboflavin (0.1%, 1.0% and 2.5%). The structure and the mechanical properties of the capsules were determined by atomic force microscopy. The effect of treatments on cell death was investigated. Collagen fibrils were well-aligned and adjacent to each other with a D-periodicity of 66.9 ± 3.2 nm and a diameter of 71.8 ± 15.4 nm in control untreated capsules. No effect of treatments was observed on the organization of the collagen fibrils nor on their intrinsic characteristics, including D-periodicity or their mean diameter. The treatments also did not induce cell death. In contrast, UV + 2.5% riboflavin induced capsule stiffness, as revealed by the increased Young’s modulus values (p < 0.0001 for each patient). Our results showed that the crosslinking procedure changed the biomechanics of diseased capsules, while keeping their structural organisation unchanged at the single fibril level. The UV/riboflavin crosslinking procedure may be a promising way to preserve the functions of collagen-based tissues and tune their elasticity for clinically relevant treatments. MDPI 2022-02-18 /pmc/articles/PMC8877509/ /pubmed/35216412 http://dx.doi.org/10.3390/ijms23042297 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cornette, Pauline
Jaabar, Ilhem Lilia
Dupres, Vincent
Werthel, Jean-David
Berenbaum, Francis
Houard, Xavier
Landoulsi, Jessem
Nourissat, Geoffroy
Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title_full Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title_fullStr Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title_full_unstemmed Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title_short Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules—Effect on Structural and Mechanical Properties
title_sort impact of collagen crosslinking on dislocated human shoulder capsules—effect on structural and mechanical properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877509/
https://www.ncbi.nlm.nih.gov/pubmed/35216412
http://dx.doi.org/10.3390/ijms23042297
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