In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm(2)). A second-harmonic-generation-to-auto-fluorescence aging index of the dermi...

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Detalles Bibliográficos
Autores principales: Zuhayri, Hala, Nikolaev, Viktor V., Knyazkova, Anastasia I., Lepekhina, Tatiana B., Krivova, Natalya A., Tuchin, Valery V., Kistenev, Yury V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877659/
https://www.ncbi.nlm.nih.gov/pubmed/35214020
http://dx.doi.org/10.3390/pharmaceutics14020287
Descripción
Sumario:The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm(2)). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60–80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm(2).

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