In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm(2)). A second-harmonic-generation-to-auto-fluorescence aging index of the dermi...

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Autores principales: Zuhayri, Hala, Nikolaev, Viktor V., Knyazkova, Anastasia I., Lepekhina, Tatiana B., Krivova, Natalya A., Tuchin, Valery V., Kistenev, Yury V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877659/
https://www.ncbi.nlm.nih.gov/pubmed/35214020
http://dx.doi.org/10.3390/pharmaceutics14020287
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author Zuhayri, Hala
Nikolaev, Viktor V.
Knyazkova, Anastasia I.
Lepekhina, Tatiana B.
Krivova, Natalya A.
Tuchin, Valery V.
Kistenev, Yury V.
author_facet Zuhayri, Hala
Nikolaev, Viktor V.
Knyazkova, Anastasia I.
Lepekhina, Tatiana B.
Krivova, Natalya A.
Tuchin, Valery V.
Kistenev, Yury V.
author_sort Zuhayri, Hala
collection PubMed
description The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm(2)). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60–80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm(2).
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spelling pubmed-88776592022-02-26 In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy Zuhayri, Hala Nikolaev, Viktor V. Knyazkova, Anastasia I. Lepekhina, Tatiana B. Krivova, Natalya A. Tuchin, Valery V. Kistenev, Yury V. Pharmaceutics Article The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm(2)). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60–80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm(2). MDPI 2022-01-26 /pmc/articles/PMC8877659/ /pubmed/35214020 http://dx.doi.org/10.3390/pharmaceutics14020287 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zuhayri, Hala
Nikolaev, Viktor V.
Knyazkova, Anastasia I.
Lepekhina, Tatiana B.
Krivova, Natalya A.
Tuchin, Valery V.
Kistenev, Yury V.
In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title_full In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title_fullStr In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title_full_unstemmed In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title_short In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy
title_sort in vivo quantification of the effectiveness of topical low-dose photodynamic therapy in wound healing using two-photon microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877659/
https://www.ncbi.nlm.nih.gov/pubmed/35214020
http://dx.doi.org/10.3390/pharmaceutics14020287
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