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Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers

Pregnancy loss predominantly occurs during the first 3–4 weeks due to fertilization failure or early embryonic losses in cattle. Insufficient biochemical communication between conceptus (embryo plus extraembryonic membranes) and endometrium has been suspected as the primary cause for early embryonic...

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Autores principales: Kusama, Kazuya, Bai, Rulan, Matsuno, Yuta, Ideta, Atsushi, Sakurai, Toshihiro, Nagaoka, Kentaro, Hori, Masatoshi, Imakawa, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877674/
https://www.ncbi.nlm.nih.gov/pubmed/35207596
http://dx.doi.org/10.3390/life12020309
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author Kusama, Kazuya
Bai, Rulan
Matsuno, Yuta
Ideta, Atsushi
Sakurai, Toshihiro
Nagaoka, Kentaro
Hori, Masatoshi
Imakawa, Kazuhiko
author_facet Kusama, Kazuya
Bai, Rulan
Matsuno, Yuta
Ideta, Atsushi
Sakurai, Toshihiro
Nagaoka, Kentaro
Hori, Masatoshi
Imakawa, Kazuhiko
author_sort Kusama, Kazuya
collection PubMed
description Pregnancy loss predominantly occurs during the first 3–4 weeks due to fertilization failure or early embryonic losses in cattle. Insufficient biochemical communication between conceptus (embryo plus extraembryonic membranes) and endometrium has been suspected as the primary cause for early embryonic losses. If molecules regulating this communication were identified, molecular mechanisms associated with early pregnancy losses could be better understood. To identify candidate molecules as detection markers of non-pregnant or females undergoing embryonic loss, peripheral blood from embryo-transferred heifers on day 7 (day 0 = day of estrus) were collected on days 17 (pre-attachment), 20 (during attachment), and 22 (post-attachment), which were subjected to metabolome and global proteome iTRAQ analyses. The metabolome analysis partly divided serum components into pregnant or not. In the iTRAQ analysis, heatmap analysis with top 25 proteins was separated into pregnant or not on day 20 or 22. Furthermore, receiver operating characteristic curve (ROC) analysis identified five candidate proteins detecting non-pregnant heifers, of which SNX5 in day 22 serum had the highest area under the curve (AUC): 0.983. We also detected SNX5 in day 22 serum from non-pregnant heifers using western blotting. These results suggest that high SNX5 in day 22 serum could predict early pregnancy loss in heifers.
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spelling pubmed-88776742022-02-26 Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers Kusama, Kazuya Bai, Rulan Matsuno, Yuta Ideta, Atsushi Sakurai, Toshihiro Nagaoka, Kentaro Hori, Masatoshi Imakawa, Kazuhiko Life (Basel) Article Pregnancy loss predominantly occurs during the first 3–4 weeks due to fertilization failure or early embryonic losses in cattle. Insufficient biochemical communication between conceptus (embryo plus extraembryonic membranes) and endometrium has been suspected as the primary cause for early embryonic losses. If molecules regulating this communication were identified, molecular mechanisms associated with early pregnancy losses could be better understood. To identify candidate molecules as detection markers of non-pregnant or females undergoing embryonic loss, peripheral blood from embryo-transferred heifers on day 7 (day 0 = day of estrus) were collected on days 17 (pre-attachment), 20 (during attachment), and 22 (post-attachment), which were subjected to metabolome and global proteome iTRAQ analyses. The metabolome analysis partly divided serum components into pregnant or not. In the iTRAQ analysis, heatmap analysis with top 25 proteins was separated into pregnant or not on day 20 or 22. Furthermore, receiver operating characteristic curve (ROC) analysis identified five candidate proteins detecting non-pregnant heifers, of which SNX5 in day 22 serum had the highest area under the curve (AUC): 0.983. We also detected SNX5 in day 22 serum from non-pregnant heifers using western blotting. These results suggest that high SNX5 in day 22 serum could predict early pregnancy loss in heifers. MDPI 2022-02-18 /pmc/articles/PMC8877674/ /pubmed/35207596 http://dx.doi.org/10.3390/life12020309 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kusama, Kazuya
Bai, Rulan
Matsuno, Yuta
Ideta, Atsushi
Sakurai, Toshihiro
Nagaoka, Kentaro
Hori, Masatoshi
Imakawa, Kazuhiko
Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title_full Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title_fullStr Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title_full_unstemmed Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title_short Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
title_sort characterization of serum metabolome and proteome profiles identifies snx5 specific for pregnancy failure in holstein heifers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877674/
https://www.ncbi.nlm.nih.gov/pubmed/35207596
http://dx.doi.org/10.3390/life12020309
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