Cargando…

Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance

Sweet sorghum is a C4 crop that can be grown for silage forage, fiber, syrup and fuel production. It is generally considered a salt-tolerant plant. However, the salt tolerance ability varies among genotypes, and the mechanism is not well known. To further uncover the salt tolerance mechanism, we per...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Chengxuan, Shang, Xiaoling, Sun, Meiyu, Tang, Sanyuan, Khan, Aimal, Zhang, Dan, Yan, Hongdong, Jiang, Yanxi, Yu, Feifei, Wu, Yaorong, Xie, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877675/
https://www.ncbi.nlm.nih.gov/pubmed/35216389
http://dx.doi.org/10.3390/ijms23042272
_version_ 1784658476474040320
author Chen, Chengxuan
Shang, Xiaoling
Sun, Meiyu
Tang, Sanyuan
Khan, Aimal
Zhang, Dan
Yan, Hongdong
Jiang, Yanxi
Yu, Feifei
Wu, Yaorong
Xie, Qi
author_facet Chen, Chengxuan
Shang, Xiaoling
Sun, Meiyu
Tang, Sanyuan
Khan, Aimal
Zhang, Dan
Yan, Hongdong
Jiang, Yanxi
Yu, Feifei
Wu, Yaorong
Xie, Qi
author_sort Chen, Chengxuan
collection PubMed
description Sweet sorghum is a C4 crop that can be grown for silage forage, fiber, syrup and fuel production. It is generally considered a salt-tolerant plant. However, the salt tolerance ability varies among genotypes, and the mechanism is not well known. To further uncover the salt tolerance mechanism, we performed comparative transcriptome analysis with RNA samples in two sweet sorghum genotypes showing different salt tolerance abilities (salt-tolerant line RIO and salt-sensitive line SN005) upon salt treatment. These response processes mainly focused on secondary metabolism, hormone signaling and stress response. The expression pattern cluster analysis showed that RIO-specific response genes were significantly enriched in the categories related to secondary metabolic pathways. GO enrichment analysis indicated that RIO responded earlier than SN005 in the 2 h after treatment. In addition, we identified more transcription factors (TFs) in RIO than SN005 that were specifically expressed differently in the first 2 h of salt treatment, and the pattern of TF change was obviously different. These results indicate that an early response in secondary metabolism might be essential for salt tolerance in sweet sorghum. In conclusion, we found that an early response, especially in secondary metabolism and hormone signaling, might be essential for salt tolerance in sweet sorghum.
format Online
Article
Text
id pubmed-8877675
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88776752022-02-26 Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance Chen, Chengxuan Shang, Xiaoling Sun, Meiyu Tang, Sanyuan Khan, Aimal Zhang, Dan Yan, Hongdong Jiang, Yanxi Yu, Feifei Wu, Yaorong Xie, Qi Int J Mol Sci Article Sweet sorghum is a C4 crop that can be grown for silage forage, fiber, syrup and fuel production. It is generally considered a salt-tolerant plant. However, the salt tolerance ability varies among genotypes, and the mechanism is not well known. To further uncover the salt tolerance mechanism, we performed comparative transcriptome analysis with RNA samples in two sweet sorghum genotypes showing different salt tolerance abilities (salt-tolerant line RIO and salt-sensitive line SN005) upon salt treatment. These response processes mainly focused on secondary metabolism, hormone signaling and stress response. The expression pattern cluster analysis showed that RIO-specific response genes were significantly enriched in the categories related to secondary metabolic pathways. GO enrichment analysis indicated that RIO responded earlier than SN005 in the 2 h after treatment. In addition, we identified more transcription factors (TFs) in RIO than SN005 that were specifically expressed differently in the first 2 h of salt treatment, and the pattern of TF change was obviously different. These results indicate that an early response in secondary metabolism might be essential for salt tolerance in sweet sorghum. In conclusion, we found that an early response, especially in secondary metabolism and hormone signaling, might be essential for salt tolerance in sweet sorghum. MDPI 2022-02-18 /pmc/articles/PMC8877675/ /pubmed/35216389 http://dx.doi.org/10.3390/ijms23042272 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Chengxuan
Shang, Xiaoling
Sun, Meiyu
Tang, Sanyuan
Khan, Aimal
Zhang, Dan
Yan, Hongdong
Jiang, Yanxi
Yu, Feifei
Wu, Yaorong
Xie, Qi
Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title_full Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title_fullStr Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title_full_unstemmed Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title_short Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance
title_sort comparative transcriptome analysis of two sweet sorghum genotypes with different salt tolerance abilities to reveal the mechanism of salt tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877675/
https://www.ncbi.nlm.nih.gov/pubmed/35216389
http://dx.doi.org/10.3390/ijms23042272
work_keys_str_mv AT chenchengxuan comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT shangxiaoling comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT sunmeiyu comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT tangsanyuan comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT khanaimal comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT zhangdan comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT yanhongdong comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT jiangyanxi comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT yufeifei comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT wuyaorong comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance
AT xieqi comparativetranscriptomeanalysisoftwosweetsorghumgenotypeswithdifferentsalttoleranceabilitiestorevealthemechanismofsalttolerance