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Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease

Leaf spot is one of the most important cassava diseases. Nanotechnology can be applied to control diseases and improve plant growth. This study was performed to prepare chitosan (CS) nanoparticle (NP)-loaded salicylic acid (SA) or silver (Ag) by the ionic gelation method, and to evaluate their effec...

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Autores principales: Hoang, Nguyen Huy, Le Thanh, Toan, Thepbandit, Wannaporn, Treekoon, Jongjit, Saengchan, Chanon, Sangpueak, Rungthip, Papathoti, Narendra Kumar, Kamkaew, Anyanee, Buensanteai, Natthiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877689/
https://www.ncbi.nlm.nih.gov/pubmed/35215572
http://dx.doi.org/10.3390/polym14040660
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author Hoang, Nguyen Huy
Le Thanh, Toan
Thepbandit, Wannaporn
Treekoon, Jongjit
Saengchan, Chanon
Sangpueak, Rungthip
Papathoti, Narendra Kumar
Kamkaew, Anyanee
Buensanteai, Natthiya
author_facet Hoang, Nguyen Huy
Le Thanh, Toan
Thepbandit, Wannaporn
Treekoon, Jongjit
Saengchan, Chanon
Sangpueak, Rungthip
Papathoti, Narendra Kumar
Kamkaew, Anyanee
Buensanteai, Natthiya
author_sort Hoang, Nguyen Huy
collection PubMed
description Leaf spot is one of the most important cassava diseases. Nanotechnology can be applied to control diseases and improve plant growth. This study was performed to prepare chitosan (CS) nanoparticle (NP)-loaded salicylic acid (SA) or silver (Ag) by the ionic gelation method, and to evaluate their effectiveness on reducing leaf spot disease and enhancing the growth of cassava plants. The CS (0.4 or 0.5%) and Pentasodium triphosphate (0.2 or 0.5%) were mixed with SA varying at 0.05, 0.1, or 0.2% or silver nitrate varying at 1, 2, or 3 mM to prepare three formulations of CS-NP-loaded SA named N1, N2, and N3 or CS-NP-loaded Ag named N4, N5, and N6. The results showed that the six formulations were not toxic to cassava leaves up to 800 ppm. The CS-NP-loaded SA (N3) and CS-NP-loaded Ag (N6) were more effective than the remaining formulations in reducing the disease severity and the disease index of leaf spot. Furthermore, N3 at 400 ppm and N6 at 200, 400, and 800 ppm could reduce disease severity (68.9–73.6% or 37.0–37.7%, depending on the time of treatment and the pathogen density) and enhance plant growth more than or equal to commercial fungicide or nano-fungicide products under net-house conditions. The study indicates the potential to use CS-NP-loaded SA or Ag as elicitors to manage cassava leaf spot disease.
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spelling pubmed-88776892022-02-26 Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease Hoang, Nguyen Huy Le Thanh, Toan Thepbandit, Wannaporn Treekoon, Jongjit Saengchan, Chanon Sangpueak, Rungthip Papathoti, Narendra Kumar Kamkaew, Anyanee Buensanteai, Natthiya Polymers (Basel) Article Leaf spot is one of the most important cassava diseases. Nanotechnology can be applied to control diseases and improve plant growth. This study was performed to prepare chitosan (CS) nanoparticle (NP)-loaded salicylic acid (SA) or silver (Ag) by the ionic gelation method, and to evaluate their effectiveness on reducing leaf spot disease and enhancing the growth of cassava plants. The CS (0.4 or 0.5%) and Pentasodium triphosphate (0.2 or 0.5%) were mixed with SA varying at 0.05, 0.1, or 0.2% or silver nitrate varying at 1, 2, or 3 mM to prepare three formulations of CS-NP-loaded SA named N1, N2, and N3 or CS-NP-loaded Ag named N4, N5, and N6. The results showed that the six formulations were not toxic to cassava leaves up to 800 ppm. The CS-NP-loaded SA (N3) and CS-NP-loaded Ag (N6) were more effective than the remaining formulations in reducing the disease severity and the disease index of leaf spot. Furthermore, N3 at 400 ppm and N6 at 200, 400, and 800 ppm could reduce disease severity (68.9–73.6% or 37.0–37.7%, depending on the time of treatment and the pathogen density) and enhance plant growth more than or equal to commercial fungicide or nano-fungicide products under net-house conditions. The study indicates the potential to use CS-NP-loaded SA or Ag as elicitors to manage cassava leaf spot disease. MDPI 2022-02-09 /pmc/articles/PMC8877689/ /pubmed/35215572 http://dx.doi.org/10.3390/polym14040660 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hoang, Nguyen Huy
Le Thanh, Toan
Thepbandit, Wannaporn
Treekoon, Jongjit
Saengchan, Chanon
Sangpueak, Rungthip
Papathoti, Narendra Kumar
Kamkaew, Anyanee
Buensanteai, Natthiya
Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title_full Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title_fullStr Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title_full_unstemmed Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title_short Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
title_sort efficacy of chitosan nanoparticle loaded-salicylic acid and -silver on management of cassava leaf spot disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877689/
https://www.ncbi.nlm.nih.gov/pubmed/35215572
http://dx.doi.org/10.3390/polym14040660
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