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Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments

Background Objectives: The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) a...

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Autores principales: Kis, Andreea M., Macasoi, Ioana, Paul, Corina, Radulescu, Matilda, Buzatu, Roxana, Watz, Claudia G., Cheveresan, Adelina, Berceanu, Delia, Pinzaru, Iulia, Dinu, Stefania, Manea, Aniko, Poenaru, Marioara, Borza, Claudia, Dehelean, Cristina A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877801/
https://www.ncbi.nlm.nih.gov/pubmed/35208492
http://dx.doi.org/10.3390/medicina58020167
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author Kis, Andreea M.
Macasoi, Ioana
Paul, Corina
Radulescu, Matilda
Buzatu, Roxana
Watz, Claudia G.
Cheveresan, Adelina
Berceanu, Delia
Pinzaru, Iulia
Dinu, Stefania
Manea, Aniko
Poenaru, Marioara
Borza, Claudia
Dehelean, Cristina A.
author_facet Kis, Andreea M.
Macasoi, Ioana
Paul, Corina
Radulescu, Matilda
Buzatu, Roxana
Watz, Claudia G.
Cheveresan, Adelina
Berceanu, Delia
Pinzaru, Iulia
Dinu, Stefania
Manea, Aniko
Poenaru, Marioara
Borza, Claudia
Dehelean, Cristina A.
author_sort Kis, Andreea M.
collection PubMed
description Background Objectives: The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) are two basic chemotherapeutics used in cancer practice, but notwithstanding despite many years of use, the mechanisms by which the multitude of side-effects occur are not yet fully understood. Thus, the present study focused on the in vitro and in ovo evaluation of the associated toxic mechanisms on keratinocytes, keys cells in the wound healing process. Materials and Methods: The two chemotherapeutics were tested in eight different concentrations to evaluate keratinocytes viability, the anti-migratory effect, and the influence on the expression of markers involved in the production of cell apoptosis. In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM). Results: The results revealed that Met induced decreased cell viability as well as increased expression of pro-apoptotic genes. In the vascular plexus of the chorioallantoic membrane, Met caused vascular irritation accompanied by capillary hemorrhage and vascular stasis. Conclusions: Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques.
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spelling pubmed-88778012022-02-26 Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments Kis, Andreea M. Macasoi, Ioana Paul, Corina Radulescu, Matilda Buzatu, Roxana Watz, Claudia G. Cheveresan, Adelina Berceanu, Delia Pinzaru, Iulia Dinu, Stefania Manea, Aniko Poenaru, Marioara Borza, Claudia Dehelean, Cristina A. Medicina (Kaunas) Article Background Objectives: The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) are two basic chemotherapeutics used in cancer practice, but notwithstanding despite many years of use, the mechanisms by which the multitude of side-effects occur are not yet fully understood. Thus, the present study focused on the in vitro and in ovo evaluation of the associated toxic mechanisms on keratinocytes, keys cells in the wound healing process. Materials and Methods: The two chemotherapeutics were tested in eight different concentrations to evaluate keratinocytes viability, the anti-migratory effect, and the influence on the expression of markers involved in the production of cell apoptosis. In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM). Results: The results revealed that Met induced decreased cell viability as well as increased expression of pro-apoptotic genes. In the vascular plexus of the chorioallantoic membrane, Met caused vascular irritation accompanied by capillary hemorrhage and vascular stasis. Conclusions: Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques. MDPI 2022-01-22 /pmc/articles/PMC8877801/ /pubmed/35208492 http://dx.doi.org/10.3390/medicina58020167 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kis, Andreea M.
Macasoi, Ioana
Paul, Corina
Radulescu, Matilda
Buzatu, Roxana
Watz, Claudia G.
Cheveresan, Adelina
Berceanu, Delia
Pinzaru, Iulia
Dinu, Stefania
Manea, Aniko
Poenaru, Marioara
Borza, Claudia
Dehelean, Cristina A.
Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title_full Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title_fullStr Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title_full_unstemmed Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title_short Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
title_sort methotrexate and cetuximab—biological impact on non-tumorigenic models: in vitro and in ovo assessments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877801/
https://www.ncbi.nlm.nih.gov/pubmed/35208492
http://dx.doi.org/10.3390/medicina58020167
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