Cargando…

Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)

Mephedrone, a synthetic cathinone, is widely abused by adolescents and young adults. The aim of this study was to determine: (i) whether prior mephedrone exposure would alter ethanol reward and (ii) whether age and matrix metalloproteinase-9 (MMP-9) are important in this regard. In our research, mal...

Descripción completa

Detalles Bibliográficos
Autores principales: Grochecki, Pawel, Smaga, Irena, Marszalek-Grabska, Marta, Lopatynska-Mazurek, Malgorzata, Slowik, Tymoteusz, Gibula-Tarlowska, Ewa, Kedzierska, Ewa, Listos, Joanna, Filip, Malgorzata, Kotlinska, Jolanta H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877998/
https://www.ncbi.nlm.nih.gov/pubmed/35216236
http://dx.doi.org/10.3390/ijms23042122
_version_ 1784658548555251712
author Grochecki, Pawel
Smaga, Irena
Marszalek-Grabska, Marta
Lopatynska-Mazurek, Malgorzata
Slowik, Tymoteusz
Gibula-Tarlowska, Ewa
Kedzierska, Ewa
Listos, Joanna
Filip, Malgorzata
Kotlinska, Jolanta H.
author_facet Grochecki, Pawel
Smaga, Irena
Marszalek-Grabska, Marta
Lopatynska-Mazurek, Malgorzata
Slowik, Tymoteusz
Gibula-Tarlowska, Ewa
Kedzierska, Ewa
Listos, Joanna
Filip, Malgorzata
Kotlinska, Jolanta H.
author_sort Grochecki, Pawel
collection PubMed
description Mephedrone, a synthetic cathinone, is widely abused by adolescents and young adults. The aim of this study was to determine: (i) whether prior mephedrone exposure would alter ethanol reward and (ii) whether age and matrix metalloproteinase-9 (MMP-9) are important in this regard. In our research, male Wistar rats at postnatal day 30 (PND30) received mephedrone at the dose of 10 mg/kg, i.p., 3 times a day for 7 days. To clarify the role of MMP-9 in the mephedrone effects, one mephedrone-treated group received minocycline, as an MMP-9 antagonist. Animals were then assigned to conditioned place preference (CPP) procedure at PND38 (adolescent) or at PND69 (adult). After the CPP test (PND48/79), expression of dopamine D1 receptors (D1R), Cav1.2 (a subtype of L-type calcium channels), and MMP-9 was quantified in the rat ventral striatum (vSTR). The influence of mephedrone administration on the N-methyl-D-aspartate glutamate receptors (NMDAR) subunits (GluN1, GluN2A, and GluN2B) was then assessed in the vSTR of adult rats (only). These results indicate that, in contrast with adolescent rats, adult rats with prior mephedrone administration appear to be more sensitive to the ethanol effect in the CPP test under the drug-free state. The mephedrone effect in adult rats was associated with upregulation of D1R, NMDAR/GluN2B, MMP-9, and Cav1.2 signaling. MMP-9 appears to contribute to these changes in proteins expression because minocycline pretreatment blocked mephedrone-evoked sensitivity to ethanol reward. Thus, our results suggest that prior mephedrone exposure differentially alters ethanol reward in adolescent and adult rats.
format Online
Article
Text
id pubmed-8877998
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88779982022-02-26 Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9) Grochecki, Pawel Smaga, Irena Marszalek-Grabska, Marta Lopatynska-Mazurek, Malgorzata Slowik, Tymoteusz Gibula-Tarlowska, Ewa Kedzierska, Ewa Listos, Joanna Filip, Malgorzata Kotlinska, Jolanta H. Int J Mol Sci Article Mephedrone, a synthetic cathinone, is widely abused by adolescents and young adults. The aim of this study was to determine: (i) whether prior mephedrone exposure would alter ethanol reward and (ii) whether age and matrix metalloproteinase-9 (MMP-9) are important in this regard. In our research, male Wistar rats at postnatal day 30 (PND30) received mephedrone at the dose of 10 mg/kg, i.p., 3 times a day for 7 days. To clarify the role of MMP-9 in the mephedrone effects, one mephedrone-treated group received minocycline, as an MMP-9 antagonist. Animals were then assigned to conditioned place preference (CPP) procedure at PND38 (adolescent) or at PND69 (adult). After the CPP test (PND48/79), expression of dopamine D1 receptors (D1R), Cav1.2 (a subtype of L-type calcium channels), and MMP-9 was quantified in the rat ventral striatum (vSTR). The influence of mephedrone administration on the N-methyl-D-aspartate glutamate receptors (NMDAR) subunits (GluN1, GluN2A, and GluN2B) was then assessed in the vSTR of adult rats (only). These results indicate that, in contrast with adolescent rats, adult rats with prior mephedrone administration appear to be more sensitive to the ethanol effect in the CPP test under the drug-free state. The mephedrone effect in adult rats was associated with upregulation of D1R, NMDAR/GluN2B, MMP-9, and Cav1.2 signaling. MMP-9 appears to contribute to these changes in proteins expression because minocycline pretreatment blocked mephedrone-evoked sensitivity to ethanol reward. Thus, our results suggest that prior mephedrone exposure differentially alters ethanol reward in adolescent and adult rats. MDPI 2022-02-14 /pmc/articles/PMC8877998/ /pubmed/35216236 http://dx.doi.org/10.3390/ijms23042122 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grochecki, Pawel
Smaga, Irena
Marszalek-Grabska, Marta
Lopatynska-Mazurek, Malgorzata
Slowik, Tymoteusz
Gibula-Tarlowska, Ewa
Kedzierska, Ewa
Listos, Joanna
Filip, Malgorzata
Kotlinska, Jolanta H.
Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title_full Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title_fullStr Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title_full_unstemmed Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title_short Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9)
title_sort alteration of ethanol reward by prior mephedrone exposure: the role of age and matrix metalloproteinase-9 (mmp-9)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877998/
https://www.ncbi.nlm.nih.gov/pubmed/35216236
http://dx.doi.org/10.3390/ijms23042122
work_keys_str_mv AT grocheckipawel alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT smagairena alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT marszalekgrabskamarta alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT lopatynskamazurekmalgorzata alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT slowiktymoteusz alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT gibulatarlowskaewa alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT kedzierskaewa alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT listosjoanna alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT filipmalgorzata alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9
AT kotlinskajolantah alterationofethanolrewardbypriormephedroneexposuretheroleofageandmatrixmetalloproteinase9mmp9