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Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton

Cyclic nucleotide-gated channels (CNGCs) constitute a family of non-selective cation channels that are primarily permeable to Ca(2+) and activated by the direct binding of cyclic nucleotides (i.e., cAMP and cGMP) to mediate cellular signaling, both in animals and plants. Until now, our understanding...

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Autores principales: Zhao, Junheng, Peng, Song, Cui, Hongtu, Li, Panyu, Li, Tianming, Liu, Luole, Zhang, Hanfeng, Tian, Zengyuan, Shang, Haihong, Xu, Ruqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878070/
https://www.ncbi.nlm.nih.gov/pubmed/35216157
http://dx.doi.org/10.3390/ijms23042041
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author Zhao, Junheng
Peng, Song
Cui, Hongtu
Li, Panyu
Li, Tianming
Liu, Luole
Zhang, Hanfeng
Tian, Zengyuan
Shang, Haihong
Xu, Ruqiang
author_facet Zhao, Junheng
Peng, Song
Cui, Hongtu
Li, Panyu
Li, Tianming
Liu, Luole
Zhang, Hanfeng
Tian, Zengyuan
Shang, Haihong
Xu, Ruqiang
author_sort Zhao, Junheng
collection PubMed
description Cyclic nucleotide-gated channels (CNGCs) constitute a family of non-selective cation channels that are primarily permeable to Ca(2+) and activated by the direct binding of cyclic nucleotides (i.e., cAMP and cGMP) to mediate cellular signaling, both in animals and plants. Until now, our understanding of CNGCs in cotton (Gossypium spp.) remains poorly addressed. In the present study, we have identified 40, 41, 20, 20, and 20 CNGC genes in G. hirsutum, G. barbadense, G. herbaceum, G. arboreum, and G. raimondii, respectively, and demonstrated characteristics of the phylogenetic relationships, gene structures, chromosomal localization, gene duplication, and synteny. Further investigation of CNGC genes in G. hirsutum, named GhCNGC1-40, indicated that they are not only extensively expressed in various tissues and at different developmental stages, but also display diverse expression patterns in response to hormones (abscisic acid, salicylic acid, methyl jasmonate, ethylene), abiotic (salt stress) and biotic (Verticillium dahlia infection) stimuli, which conform with a variety of cis-acting regulatory elements residing in the promoter regions; moreover, a set of GhCNGCs are responsive to cAMP signaling during cotton fiber development. Protein–protein interactions supported the functional aspects of GhCNGCs in plant growth, development, and stress responses. Accordingly, the silencing of the homoeologous gene pair GhCNGC1&18 and GhCNGC12&31 impaired plant growth and development; however, GhCNGC1&18-silenced plants enhanced Verticillium wilt resistance and salt tolerance, whereas GhCNGC12&31-silenced plants had opposite effects. Together, these results unveiled the dynamic expression, differential regulation, and functional diversity of the CNGC family genes in cotton. The present work has laid the foundation for further studies and the utilization of CNGCs in cotton genetic improvement.
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spelling pubmed-88780702022-02-26 Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton Zhao, Junheng Peng, Song Cui, Hongtu Li, Panyu Li, Tianming Liu, Luole Zhang, Hanfeng Tian, Zengyuan Shang, Haihong Xu, Ruqiang Int J Mol Sci Article Cyclic nucleotide-gated channels (CNGCs) constitute a family of non-selective cation channels that are primarily permeable to Ca(2+) and activated by the direct binding of cyclic nucleotides (i.e., cAMP and cGMP) to mediate cellular signaling, both in animals and plants. Until now, our understanding of CNGCs in cotton (Gossypium spp.) remains poorly addressed. In the present study, we have identified 40, 41, 20, 20, and 20 CNGC genes in G. hirsutum, G. barbadense, G. herbaceum, G. arboreum, and G. raimondii, respectively, and demonstrated characteristics of the phylogenetic relationships, gene structures, chromosomal localization, gene duplication, and synteny. Further investigation of CNGC genes in G. hirsutum, named GhCNGC1-40, indicated that they are not only extensively expressed in various tissues and at different developmental stages, but also display diverse expression patterns in response to hormones (abscisic acid, salicylic acid, methyl jasmonate, ethylene), abiotic (salt stress) and biotic (Verticillium dahlia infection) stimuli, which conform with a variety of cis-acting regulatory elements residing in the promoter regions; moreover, a set of GhCNGCs are responsive to cAMP signaling during cotton fiber development. Protein–protein interactions supported the functional aspects of GhCNGCs in plant growth, development, and stress responses. Accordingly, the silencing of the homoeologous gene pair GhCNGC1&18 and GhCNGC12&31 impaired plant growth and development; however, GhCNGC1&18-silenced plants enhanced Verticillium wilt resistance and salt tolerance, whereas GhCNGC12&31-silenced plants had opposite effects. Together, these results unveiled the dynamic expression, differential regulation, and functional diversity of the CNGC family genes in cotton. The present work has laid the foundation for further studies and the utilization of CNGCs in cotton genetic improvement. MDPI 2022-02-12 /pmc/articles/PMC8878070/ /pubmed/35216157 http://dx.doi.org/10.3390/ijms23042041 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Junheng
Peng, Song
Cui, Hongtu
Li, Panyu
Li, Tianming
Liu, Luole
Zhang, Hanfeng
Tian, Zengyuan
Shang, Haihong
Xu, Ruqiang
Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title_full Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title_fullStr Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title_full_unstemmed Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title_short Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton
title_sort dynamic expression, differential regulation and functional diversity of the cngc family genes in cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878070/
https://www.ncbi.nlm.nih.gov/pubmed/35216157
http://dx.doi.org/10.3390/ijms23042041
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