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Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)

This study aimed to test the biocompatibility and antibacterial properties of Salvadora persica (S. persica) extract, a natural product, as an intracanal medication in comparison with calcium hydroxide (Metapaste, META BIOMED, Cheongju, Korea). The pH values of both materials were tested. The biocom...

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Autores principales: Abdeltawab, Samah Samir, Abu Haimed, Tariq S., Bahammam, Hammam Ahmed, Arab, Wafaa Talat, Abou Neel, Ensanya A., Bahammam, Laila Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878108/
https://www.ncbi.nlm.nih.gov/pubmed/35207914
http://dx.doi.org/10.3390/ma15041373
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author Abdeltawab, Samah Samir
Abu Haimed, Tariq S.
Bahammam, Hammam Ahmed
Arab, Wafaa Talat
Abou Neel, Ensanya A.
Bahammam, Laila Ahmed
author_facet Abdeltawab, Samah Samir
Abu Haimed, Tariq S.
Bahammam, Hammam Ahmed
Arab, Wafaa Talat
Abou Neel, Ensanya A.
Bahammam, Laila Ahmed
author_sort Abdeltawab, Samah Samir
collection PubMed
description This study aimed to test the biocompatibility and antibacterial properties of Salvadora persica (S. persica) extract, a natural product, as an intracanal medication in comparison with calcium hydroxide (Metapaste, META BIOMED, Cheongju, Korea). The pH values of both materials were tested. The biocompatibility of S. persica extract and Metapaste was determined using light microscopy and MTT assays. The antibacterial action was tested using the zone of bacterial inhibition on four common bacterial species. In addition, intracanal medication was administered using 68 extracted single-rooted teeth contaminated with Enterococcus faecalis (E. faecalis), and the percentage reduction in colony count (% RCC) at 1, 3, and 7 days was measured. The extension of activity for both materials was assessed using histological sections and scanning electron microscopy. S. persica was found to be acidic in nature. Both materials showed significantly lower cell viability than the positive control cells on days 1 and 3 but not on day 7. S. persica showed better antibacterial effects against E. faecalis and S. mutans. S. persica extract showed 97.6%, 98.9%, and 99.3% RCC values at 1, 3, and 7 days, respectively, which are comparable to those of Metapaste. S. persica herbal extract is a promising material that can be utilized as an intracanal medication, but its use requires further research.
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spelling pubmed-88781082022-02-26 Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment) Abdeltawab, Samah Samir Abu Haimed, Tariq S. Bahammam, Hammam Ahmed Arab, Wafaa Talat Abou Neel, Ensanya A. Bahammam, Laila Ahmed Materials (Basel) Article This study aimed to test the biocompatibility and antibacterial properties of Salvadora persica (S. persica) extract, a natural product, as an intracanal medication in comparison with calcium hydroxide (Metapaste, META BIOMED, Cheongju, Korea). The pH values of both materials were tested. The biocompatibility of S. persica extract and Metapaste was determined using light microscopy and MTT assays. The antibacterial action was tested using the zone of bacterial inhibition on four common bacterial species. In addition, intracanal medication was administered using 68 extracted single-rooted teeth contaminated with Enterococcus faecalis (E. faecalis), and the percentage reduction in colony count (% RCC) at 1, 3, and 7 days was measured. The extension of activity for both materials was assessed using histological sections and scanning electron microscopy. S. persica was found to be acidic in nature. Both materials showed significantly lower cell viability than the positive control cells on days 1 and 3 but not on day 7. S. persica showed better antibacterial effects against E. faecalis and S. mutans. S. persica extract showed 97.6%, 98.9%, and 99.3% RCC values at 1, 3, and 7 days, respectively, which are comparable to those of Metapaste. S. persica herbal extract is a promising material that can be utilized as an intracanal medication, but its use requires further research. MDPI 2022-02-12 /pmc/articles/PMC8878108/ /pubmed/35207914 http://dx.doi.org/10.3390/ma15041373 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdeltawab, Samah Samir
Abu Haimed, Tariq S.
Bahammam, Hammam Ahmed
Arab, Wafaa Talat
Abou Neel, Ensanya A.
Bahammam, Laila Ahmed
Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title_full Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title_fullStr Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title_full_unstemmed Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title_short Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)
title_sort biocompatibility and antibacterial action of salvadora persica extract as intracanal medication (in vitro and ex vivo experiment)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878108/
https://www.ncbi.nlm.nih.gov/pubmed/35207914
http://dx.doi.org/10.3390/ma15041373
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