Biodiversity of Forensically Relevant Blowflies (Diptera: Calliphoridae) at the Anthropology Research Facility in Knoxville, Tennessee, USA

SIMPLE SUMMARY: This study investigates the biodiversity and seasonal distribution of blowflies at the Anthropology Research Facility (ARF) of the University of Tennessee, which is also known as the “Body Farm”. Blowflies are among the first insects that access dead bodies, and have a significant impact on the rate and pattern of decomposition. Although the ARF has been used for numerous taphonomic and entomological studies over the past 40 years, it is not yet fully known what blowfly species are present in the area. After a 14-month-long blowfly survey at the ARF, we collected a total of 3180 adult blowflies, comprising 13 species from 7 genera. Phormia regina (Meigen) and Lucilia coeruleiviridis (Macquart) were the predominant species collected from this survey, representing 65.9% and 20.6% of collections, respectively. Among the 13 species, Protophormia terraenovae (Robineau-Desvoidy) was collected for the first time in Tennessee. In addition to relative abundance, we also investigated blowfly community composition, species abundance, richness, and diversity by season. This research is expected to provide researchers at the ARF with accurate information about the blowflies so that they can plan and design their research accordingly. ABSTRACT: Understanding the biodiversity and distribution of forensically relevant blowflies (Diptera: Calliphoridae) in a region can aid in legal investigations when insects are associated with remains. For this purpose, we conducted a 14-month-long blowfly survey at the Anthropology Research Facility (ARF) of the University of Tennessee in Knoxville, Tennessee. Traps baited with pork kidney were deployed for 24 h twice a month throughout the study. A total of 3180 adult blowflies were collected, comprising 13 species from 7 genera. Phormia regina (Meigen) and Lucilia coeruleiviridis (Macquart) were the predominant species collected from this survey, with collections representing 65.9% and 20.6%of total flies captured, respectively. In addition to relative abundance, we investigated blowfly community composition, species abundance, richness, and diversity by season. One state record was identified, with adult Protophormia terraenovae (Robineau-Desvoidy) being collected for the first time in Tennessee. Additionally, an earlier record of Chrysomya megacephala (Fabricius) in Tennessee was noted. These findings can be used to aid in legal investigations in the area and surrounding areas where work is limited, as well as to provide information on which forensically relevant species should be the subject of future research in the area.