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Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug...

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Autores principales: Pliego-Cortés, Hugo, Hardouin, Kévin, Bedoux, Gilles, Marty, Christel, Cérantola, Stéphane, Freile-Pelegrín, Yolanda, Robledo, Daniel, Bourgougnon, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878361/
https://www.ncbi.nlm.nih.gov/pubmed/35200645
http://dx.doi.org/10.3390/md20020116
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author Pliego-Cortés, Hugo
Hardouin, Kévin
Bedoux, Gilles
Marty, Christel
Cérantola, Stéphane
Freile-Pelegrín, Yolanda
Robledo, Daniel
Bourgougnon, Nathalie
author_facet Pliego-Cortés, Hugo
Hardouin, Kévin
Bedoux, Gilles
Marty, Christel
Cérantola, Stéphane
Freile-Pelegrín, Yolanda
Robledo, Daniel
Bourgougnon, Nathalie
author_sort Pliego-Cortés, Hugo
collection PubMed
description Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC(50) 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC(50) of 0.38 μg/mL during the viral adsorption assays when the polysaccharide and the virus were added simultaneously, whilst the protection on Vero cell during the post-infection assay was effective up to 1 h. The chemical composition, FTIR and (1)H NMR spectroscopic, and molecular weights of the sr-SPs from H. floresii were determined and discussed based on the anti-herpetic activity. The potential utilization of seaweed stranding as a source of antiviral compounds is addressed.
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spelling pubmed-88783612022-02-26 Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1 Pliego-Cortés, Hugo Hardouin, Kévin Bedoux, Gilles Marty, Christel Cérantola, Stéphane Freile-Pelegrín, Yolanda Robledo, Daniel Bourgougnon, Nathalie Mar Drugs Article Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC(50) 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC(50) of 0.38 μg/mL during the viral adsorption assays when the polysaccharide and the virus were added simultaneously, whilst the protection on Vero cell during the post-infection assay was effective up to 1 h. The chemical composition, FTIR and (1)H NMR spectroscopic, and molecular weights of the sr-SPs from H. floresii were determined and discussed based on the anti-herpetic activity. The potential utilization of seaweed stranding as a source of antiviral compounds is addressed. MDPI 2022-02-01 /pmc/articles/PMC8878361/ /pubmed/35200645 http://dx.doi.org/10.3390/md20020116 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pliego-Cortés, Hugo
Hardouin, Kévin
Bedoux, Gilles
Marty, Christel
Cérantola, Stéphane
Freile-Pelegrín, Yolanda
Robledo, Daniel
Bourgougnon, Nathalie
Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title_full Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title_fullStr Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title_full_unstemmed Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title_short Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1
title_sort sulfated polysaccharides from seaweed strandings as renewable source for potential antivirals against herpes simplex virus 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878361/
https://www.ncbi.nlm.nih.gov/pubmed/35200645
http://dx.doi.org/10.3390/md20020116
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