Cargando…

Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands

Macroalgae-associated bacteria play an important role in their algal hosts’ biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structures and functions between epi- and endo- bacteria, an ef...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiaojie, Zhao, Jin, Jiang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878427/
https://www.ncbi.nlm.nih.gov/pubmed/35208930
http://dx.doi.org/10.3390/microorganisms10020476
_version_ 1784658657891319808
author Liu, Xiaojie
Zhao, Jin
Jiang, Peng
author_facet Liu, Xiaojie
Zhao, Jin
Jiang, Peng
author_sort Liu, Xiaojie
collection PubMed
description Macroalgae-associated bacteria play an important role in their algal hosts’ biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structures and functions between epi- and endo- bacteria, an effective approach for maximizing epiphyte removal from delicate seaweeds while retaining endophyte fidelity must be developed. In this study, a variety of surface sterilization methods for Ulva prolifera were compared, including mechanical, chemical, and enzymatical treatments. According to the results of scanning electron microscope (SEM) and denaturing gradient gel electrophoresis (DGGE) analysis, almost complete removal of epiphytic bacteria on Ulva was obtained simply by co-vortex of seaweeds with silica sands, causing minimal disturbance to endosymbionts when compared to previous published methods. In addition, the adaptability was also confirmed in additional U. prolifera strains and Ulva species with blade-like or narrow tubular thallus shapes. This easy mechanical method would enable the analysis of community composition and host specificity for Ulva-associated epi- and endo-bacteria separately.
format Online
Article
Text
id pubmed-8878427
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88784272022-02-26 Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands Liu, Xiaojie Zhao, Jin Jiang, Peng Microorganisms Technical Note Macroalgae-associated bacteria play an important role in their algal hosts’ biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structures and functions between epi- and endo- bacteria, an effective approach for maximizing epiphyte removal from delicate seaweeds while retaining endophyte fidelity must be developed. In this study, a variety of surface sterilization methods for Ulva prolifera were compared, including mechanical, chemical, and enzymatical treatments. According to the results of scanning electron microscope (SEM) and denaturing gradient gel electrophoresis (DGGE) analysis, almost complete removal of epiphytic bacteria on Ulva was obtained simply by co-vortex of seaweeds with silica sands, causing minimal disturbance to endosymbionts when compared to previous published methods. In addition, the adaptability was also confirmed in additional U. prolifera strains and Ulva species with blade-like or narrow tubular thallus shapes. This easy mechanical method would enable the analysis of community composition and host specificity for Ulva-associated epi- and endo-bacteria separately. MDPI 2022-02-21 /pmc/articles/PMC8878427/ /pubmed/35208930 http://dx.doi.org/10.3390/microorganisms10020476 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Liu, Xiaojie
Zhao, Jin
Jiang, Peng
Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title_full Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title_fullStr Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title_full_unstemmed Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title_short Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
title_sort easy removal of epiphytic bacteria on ulva (ulvophyceae, chlorophyta) by vortex with silica sands
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878427/
https://www.ncbi.nlm.nih.gov/pubmed/35208930
http://dx.doi.org/10.3390/microorganisms10020476
work_keys_str_mv AT liuxiaojie easyremovalofepiphyticbacteriaonulvaulvophyceaechlorophytabyvortexwithsilicasands
AT zhaojin easyremovalofepiphyticbacteriaonulvaulvophyceaechlorophytabyvortexwithsilicasands
AT jiangpeng easyremovalofepiphyticbacteriaonulvaulvophyceaechlorophytabyvortexwithsilicasands