Cargando…
Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome
Multiple sclerosis (MS) is a neurological disorder of autoimmune aetiology. Experimental therapies with the use of mesenchymal stem cells (MSCs) have emerged as a response to the unmet need for new treatment options. The unique immunomodulatory features of stem cells obtained from Wharton’s jelly (W...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878514/ https://www.ncbi.nlm.nih.gov/pubmed/35216294 http://dx.doi.org/10.3390/ijms23042177 |
_version_ | 1784658678435020800 |
---|---|
author | Salwierak-Głośna, Karolina Piątek, Paweł Domowicz, Małgorzata Świderek-Matysiak, Mariola |
author_facet | Salwierak-Głośna, Karolina Piątek, Paweł Domowicz, Małgorzata Świderek-Matysiak, Mariola |
author_sort | Salwierak-Głośna, Karolina |
collection | PubMed |
description | Multiple sclerosis (MS) is a neurological disorder of autoimmune aetiology. Experimental therapies with the use of mesenchymal stem cells (MSCs) have emerged as a response to the unmet need for new treatment options. The unique immunomodulatory features of stem cells obtained from Wharton’s jelly (WJ-MSCs) make them an interesting research and therapeutic model. Most WJ-MSCs transplants for multiple sclerosis use intrathecal administration. We studied the effect of cerebrospinal fluid (CSF) obtained from MS patients on the secretory activity of WJ-MSCs and broaden this observation with WJ-MSCs interactions with human oligodendroglia cell line (OLs). Analysis of the WJ-MSCs secretory activity with use of Bio-Plex Pro™ Human Cytokine confirmed significant and diverse immunomodulatory potential. Our data reveal rich WJ-MSCs secretome with markedly increased levels of IL-6, IL-8, IP-10 and MCP-1 synthesis and a favourable profile of growth factors. The addition of MS CSF to the WJ-MSCs culture caused depletion of most proteins measured, only IL-12, RANTES and GM-CSF levels were increased. Most cytokines and chemokines decreased their concentrations in WJ-MSCs co-cultured with OLs, only eotaxin and RANTES levels were slightly increased. These results emphasize the spectrum of the immunomodulatory properties of WJ-MSCs and show how those effects can be modulated depending on the transplantation milieu. |
format | Online Article Text |
id | pubmed-8878514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88785142022-02-26 Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Salwierak-Głośna, Karolina Piątek, Paweł Domowicz, Małgorzata Świderek-Matysiak, Mariola Int J Mol Sci Article Multiple sclerosis (MS) is a neurological disorder of autoimmune aetiology. Experimental therapies with the use of mesenchymal stem cells (MSCs) have emerged as a response to the unmet need for new treatment options. The unique immunomodulatory features of stem cells obtained from Wharton’s jelly (WJ-MSCs) make them an interesting research and therapeutic model. Most WJ-MSCs transplants for multiple sclerosis use intrathecal administration. We studied the effect of cerebrospinal fluid (CSF) obtained from MS patients on the secretory activity of WJ-MSCs and broaden this observation with WJ-MSCs interactions with human oligodendroglia cell line (OLs). Analysis of the WJ-MSCs secretory activity with use of Bio-Plex Pro™ Human Cytokine confirmed significant and diverse immunomodulatory potential. Our data reveal rich WJ-MSCs secretome with markedly increased levels of IL-6, IL-8, IP-10 and MCP-1 synthesis and a favourable profile of growth factors. The addition of MS CSF to the WJ-MSCs culture caused depletion of most proteins measured, only IL-12, RANTES and GM-CSF levels were increased. Most cytokines and chemokines decreased their concentrations in WJ-MSCs co-cultured with OLs, only eotaxin and RANTES levels were slightly increased. These results emphasize the spectrum of the immunomodulatory properties of WJ-MSCs and show how those effects can be modulated depending on the transplantation milieu. MDPI 2022-02-16 /pmc/articles/PMC8878514/ /pubmed/35216294 http://dx.doi.org/10.3390/ijms23042177 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Salwierak-Głośna, Karolina Piątek, Paweł Domowicz, Małgorzata Świderek-Matysiak, Mariola Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title | Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title_full | Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title_fullStr | Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title_full_unstemmed | Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title_short | Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome |
title_sort | effect of multiple sclerosis cerebrospinal fluid and oligodendroglia cell line environment on human wharton’s jelly mesenchymal stem cells secretome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878514/ https://www.ncbi.nlm.nih.gov/pubmed/35216294 http://dx.doi.org/10.3390/ijms23042177 |
work_keys_str_mv | AT salwierakgłosnakarolina effectofmultiplesclerosiscerebrospinalfluidandoligodendrogliacelllineenvironmentonhumanwhartonsjellymesenchymalstemcellssecretome AT piatekpaweł effectofmultiplesclerosiscerebrospinalfluidandoligodendrogliacelllineenvironmentonhumanwhartonsjellymesenchymalstemcellssecretome AT domowiczmałgorzata effectofmultiplesclerosiscerebrospinalfluidandoligodendrogliacelllineenvironmentonhumanwhartonsjellymesenchymalstemcellssecretome AT swiderekmatysiakmariola effectofmultiplesclerosiscerebrospinalfluidandoligodendrogliacelllineenvironmentonhumanwhartonsjellymesenchymalstemcellssecretome |