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Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland

The BK polyomavirus (BKPyV) is a widespread pathogen in humans. Polymorphism of the region encoding the VP1 protein of BKPyV provides the basis for classifying the virus into types and subtypes, whose frequency varies depending on geographic location. The aim of our study was to determine the freque...

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Autores principales: Furmaga, Jacek, Kowalczyk, Marek, Furmaga, Olga, Rokos, Christos A., Zapolski, Tomasz, Krakowski, Leszek, Jakubczak, Andrzej, Rudzki, Sławomir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878621/
https://www.ncbi.nlm.nih.gov/pubmed/35215804
http://dx.doi.org/10.3390/v14020209
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author Furmaga, Jacek
Kowalczyk, Marek
Furmaga, Olga
Rokos, Christos A.
Zapolski, Tomasz
Krakowski, Leszek
Jakubczak, Andrzej
Rudzki, Sławomir
author_facet Furmaga, Jacek
Kowalczyk, Marek
Furmaga, Olga
Rokos, Christos A.
Zapolski, Tomasz
Krakowski, Leszek
Jakubczak, Andrzej
Rudzki, Sławomir
author_sort Furmaga, Jacek
collection PubMed
description The BK polyomavirus (BKPyV) is a widespread pathogen in humans. Polymorphism of the region encoding the VP1 protein of BKPyV provides the basis for classifying the virus into types and subtypes, whose frequency varies depending on geographic location. The aim of our study was to determine the frequency of BKPyV in the Polish population and to assess its variation by analysing polymorphism in the typing region. The study was conducted on 168 healthy, Polish volunteers, whose blood (plasma) and urine were sampled. The virus was detected using PCR, products, sequenced and subjected to bioinformatic analysis. In addition, viral load was assessed by qPCR. The presence of the genetic material of the BK virus was noted in 61/168 urine samples but in none of the plasma sample. Sequencing and phylogenetic analysis confirmed that the BKPyV isolates were of types I and IV, dominant in Europe (63.93% and 36.07%, respectively). All isolates from genotype I belonged to subtype Ib-2, showing polymorphism at position 1809 with a frequency of 61.54% (G1809A) and 38.46% (G1809C). To the best of our knowledge, this is the first study of this magnitude on the genetic variation of BKPyV among healthy volunteers in Poland.
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spelling pubmed-88786212022-02-26 Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland Furmaga, Jacek Kowalczyk, Marek Furmaga, Olga Rokos, Christos A. Zapolski, Tomasz Krakowski, Leszek Jakubczak, Andrzej Rudzki, Sławomir Viruses Article The BK polyomavirus (BKPyV) is a widespread pathogen in humans. Polymorphism of the region encoding the VP1 protein of BKPyV provides the basis for classifying the virus into types and subtypes, whose frequency varies depending on geographic location. The aim of our study was to determine the frequency of BKPyV in the Polish population and to assess its variation by analysing polymorphism in the typing region. The study was conducted on 168 healthy, Polish volunteers, whose blood (plasma) and urine were sampled. The virus was detected using PCR, products, sequenced and subjected to bioinformatic analysis. In addition, viral load was assessed by qPCR. The presence of the genetic material of the BK virus was noted in 61/168 urine samples but in none of the plasma sample. Sequencing and phylogenetic analysis confirmed that the BKPyV isolates were of types I and IV, dominant in Europe (63.93% and 36.07%, respectively). All isolates from genotype I belonged to subtype Ib-2, showing polymorphism at position 1809 with a frequency of 61.54% (G1809A) and 38.46% (G1809C). To the best of our knowledge, this is the first study of this magnitude on the genetic variation of BKPyV among healthy volunteers in Poland. MDPI 2022-01-21 /pmc/articles/PMC8878621/ /pubmed/35215804 http://dx.doi.org/10.3390/v14020209 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Furmaga, Jacek
Kowalczyk, Marek
Furmaga, Olga
Rokos, Christos A.
Zapolski, Tomasz
Krakowski, Leszek
Jakubczak, Andrzej
Rudzki, Sławomir
Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title_full Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title_fullStr Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title_full_unstemmed Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title_short Molecular Epidemiology and Variation of the BK Polyomavirus in the Population of Central and Eastern Europe Based on the Example of Poland
title_sort molecular epidemiology and variation of the bk polyomavirus in the population of central and eastern europe based on the example of poland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878621/
https://www.ncbi.nlm.nih.gov/pubmed/35215804
http://dx.doi.org/10.3390/v14020209
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