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Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI;...

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Autores principales: Fernandes, Mileni S., Sabino-Arias, Isabela T., Dionizio, Aline, Fabricio, Mayara F., Trevizol, Juliana S., Martini, Tatiana, Azevedo, Liane B., Valentine, Ruth A., Maguire, Anne, Zohoori, Fatemeh V., L. Amaral, Sandra, Buzalaf, Marília A. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878675/
https://www.ncbi.nlm.nih.gov/pubmed/35208192
http://dx.doi.org/10.3390/metabo12020117
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author Fernandes, Mileni S.
Sabino-Arias, Isabela T.
Dionizio, Aline
Fabricio, Mayara F.
Trevizol, Juliana S.
Martini, Tatiana
Azevedo, Liane B.
Valentine, Ruth A.
Maguire, Anne
Zohoori, Fatemeh V.
L. Amaral, Sandra
Buzalaf, Marília A. R.
author_facet Fernandes, Mileni S.
Sabino-Arias, Isabela T.
Dionizio, Aline
Fabricio, Mayara F.
Trevizol, Juliana S.
Martini, Tatiana
Azevedo, Liane B.
Valentine, Ruth A.
Maguire, Anne
Zohoori, Fatemeh V.
L. Amaral, Sandra
Buzalaf, Marília A. R.
author_sort Fernandes, Mileni S.
collection PubMed
description We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI; RI) No-F, No-Exercise, (SII; RII) 50 ppm F, No-Exercise, (SIII; RIII) 50 ppm F, Exercise. Overall, mean F concentrations in the plasma and femur were significantly higher in R-mice compared with S-mice. In R-mice, exercise resulted in an increase in F accumulation in the femur. In S-mice, the mean plasma glucose level was significantly higher in Group II compared with Groups I and III. There was an increase in liver proteins involved in energy flux and antioxidant enzymes in non-exercise groups (I, II) of S-mice in comparison with the corresponding groups of R-mice. The results also showed a decrease in muscle protein expression in Group I S-mice compared with their R-mice counterparts. In conclusion, the findings suggest an increased state of oxidative stress in fluorosis-susceptible mice that might be exacerbated by the treatment with F. In addition, fluorosis-susceptible mice have plasma glucose levels higher than fluorosis-resistant mice on exposure to F, and this is not affected by exercise.
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spelling pubmed-88786752022-02-26 Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice Fernandes, Mileni S. Sabino-Arias, Isabela T. Dionizio, Aline Fabricio, Mayara F. Trevizol, Juliana S. Martini, Tatiana Azevedo, Liane B. Valentine, Ruth A. Maguire, Anne Zohoori, Fatemeh V. L. Amaral, Sandra Buzalaf, Marília A. R. Metabolites Article We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI; RI) No-F, No-Exercise, (SII; RII) 50 ppm F, No-Exercise, (SIII; RIII) 50 ppm F, Exercise. Overall, mean F concentrations in the plasma and femur were significantly higher in R-mice compared with S-mice. In R-mice, exercise resulted in an increase in F accumulation in the femur. In S-mice, the mean plasma glucose level was significantly higher in Group II compared with Groups I and III. There was an increase in liver proteins involved in energy flux and antioxidant enzymes in non-exercise groups (I, II) of S-mice in comparison with the corresponding groups of R-mice. The results also showed a decrease in muscle protein expression in Group I S-mice compared with their R-mice counterparts. In conclusion, the findings suggest an increased state of oxidative stress in fluorosis-susceptible mice that might be exacerbated by the treatment with F. In addition, fluorosis-susceptible mice have plasma glucose levels higher than fluorosis-resistant mice on exposure to F, and this is not affected by exercise. MDPI 2022-01-25 /pmc/articles/PMC8878675/ /pubmed/35208192 http://dx.doi.org/10.3390/metabo12020117 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandes, Mileni S.
Sabino-Arias, Isabela T.
Dionizio, Aline
Fabricio, Mayara F.
Trevizol, Juliana S.
Martini, Tatiana
Azevedo, Liane B.
Valentine, Ruth A.
Maguire, Anne
Zohoori, Fatemeh V.
L. Amaral, Sandra
Buzalaf, Marília A. R.
Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title_full Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title_fullStr Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title_full_unstemmed Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title_short Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice
title_sort effect of physical exercise and genetic background on glucose homeostasis and liver/muscle proteomes in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878675/
https://www.ncbi.nlm.nih.gov/pubmed/35208192
http://dx.doi.org/10.3390/metabo12020117
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