Cargando…

Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses

We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confi...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Guanrong, Zhang, Jinyong, Zhang, Ying, Li, Chenghui, Zhang, Duo, Li, Yiquan, Zhou, Hongning, Li, Nan, Xiao, Pengpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878809/
https://www.ncbi.nlm.nih.gov/pubmed/35223559
http://dx.doi.org/10.3389/fcimb.2022.849662
_version_ 1784658748705341440
author Feng, Guanrong
Zhang, Jinyong
Zhang, Ying
Li, Chenghui
Zhang, Duo
Li, Yiquan
Zhou, Hongning
Li, Nan
Xiao, Pengpeng
author_facet Feng, Guanrong
Zhang, Jinyong
Zhang, Ying
Li, Chenghui
Zhang, Duo
Li, Yiquan
Zhou, Hongning
Li, Nan
Xiao, Pengpeng
author_sort Feng, Guanrong
collection PubMed
description We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confirm the results, followed by phylogenetic analysis. Herein, part of mosquito virome was identified, including chikungunya virus (CHIKV), Getah virus (GETV), and Ross river virus (RRV). After metagenomic analysis, seven CHIKV sequences were verified by PCR amplification, among which CHIKV-China/YN2018-1 had the highest homology with the CHIKV isolated in Senegal, 1983, with a nucleotide (nt) identity of at least 81%, belonging to genotype West Africa viral genes. Five GETV sequences were identified, which had a high homology with the GETV sequences isolated from Equus caballus in Japan, 1978, with a (nt) identity of at least 97%. The newly isolated virus CHIKV-China/YN2018-1 became more infectious after passage of the BHK-21 cell line to the Vero cell line. The newly identified RRV gene had the highest homology with the 2006 RRV isolate from Australia, with a (nt) identity of at least 94%. In addition, numerous known and unknown viruses have also been detected in mosquitoes from Yunnan province, China, and propagation tests will be carried out.
format Online
Article
Text
id pubmed-8878809
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88788092022-02-26 Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses Feng, Guanrong Zhang, Jinyong Zhang, Ying Li, Chenghui Zhang, Duo Li, Yiquan Zhou, Hongning Li, Nan Xiao, Pengpeng Front Cell Infect Microbiol Cellular and Infection Microbiology We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confirm the results, followed by phylogenetic analysis. Herein, part of mosquito virome was identified, including chikungunya virus (CHIKV), Getah virus (GETV), and Ross river virus (RRV). After metagenomic analysis, seven CHIKV sequences were verified by PCR amplification, among which CHIKV-China/YN2018-1 had the highest homology with the CHIKV isolated in Senegal, 1983, with a nucleotide (nt) identity of at least 81%, belonging to genotype West Africa viral genes. Five GETV sequences were identified, which had a high homology with the GETV sequences isolated from Equus caballus in Japan, 1978, with a (nt) identity of at least 97%. The newly isolated virus CHIKV-China/YN2018-1 became more infectious after passage of the BHK-21 cell line to the Vero cell line. The newly identified RRV gene had the highest homology with the 2006 RRV isolate from Australia, with a (nt) identity of at least 94%. In addition, numerous known and unknown viruses have also been detected in mosquitoes from Yunnan province, China, and propagation tests will be carried out. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8878809/ /pubmed/35223559 http://dx.doi.org/10.3389/fcimb.2022.849662 Text en Copyright © 2022 Feng, Zhang, Zhang, Li, Zhang, Li, Zhou, Li and Xiao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Feng, Guanrong
Zhang, Jinyong
Zhang, Ying
Li, Chenghui
Zhang, Duo
Li, Yiquan
Zhou, Hongning
Li, Nan
Xiao, Pengpeng
Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title_full Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title_fullStr Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title_full_unstemmed Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title_short Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
title_sort metagenomic analysis of togaviridae in mosquito viromes isolated from yunnan province in china reveals genes from chikungunya and ross river viruses
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878809/
https://www.ncbi.nlm.nih.gov/pubmed/35223559
http://dx.doi.org/10.3389/fcimb.2022.849662
work_keys_str_mv AT fengguanrong metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT zhangjinyong metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT zhangying metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT lichenghui metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT zhangduo metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT liyiquan metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT zhouhongning metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT linan metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses
AT xiaopengpeng metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses