Cargando…
Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses
We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878809/ https://www.ncbi.nlm.nih.gov/pubmed/35223559 http://dx.doi.org/10.3389/fcimb.2022.849662 |
_version_ | 1784658748705341440 |
---|---|
author | Feng, Guanrong Zhang, Jinyong Zhang, Ying Li, Chenghui Zhang, Duo Li, Yiquan Zhou, Hongning Li, Nan Xiao, Pengpeng |
author_facet | Feng, Guanrong Zhang, Jinyong Zhang, Ying Li, Chenghui Zhang, Duo Li, Yiquan Zhou, Hongning Li, Nan Xiao, Pengpeng |
author_sort | Feng, Guanrong |
collection | PubMed |
description | We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confirm the results, followed by phylogenetic analysis. Herein, part of mosquito virome was identified, including chikungunya virus (CHIKV), Getah virus (GETV), and Ross river virus (RRV). After metagenomic analysis, seven CHIKV sequences were verified by PCR amplification, among which CHIKV-China/YN2018-1 had the highest homology with the CHIKV isolated in Senegal, 1983, with a nucleotide (nt) identity of at least 81%, belonging to genotype West Africa viral genes. Five GETV sequences were identified, which had a high homology with the GETV sequences isolated from Equus caballus in Japan, 1978, with a (nt) identity of at least 97%. The newly isolated virus CHIKV-China/YN2018-1 became more infectious after passage of the BHK-21 cell line to the Vero cell line. The newly identified RRV gene had the highest homology with the 2006 RRV isolate from Australia, with a (nt) identity of at least 94%. In addition, numerous known and unknown viruses have also been detected in mosquitoes from Yunnan province, China, and propagation tests will be carried out. |
format | Online Article Text |
id | pubmed-8878809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88788092022-02-26 Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses Feng, Guanrong Zhang, Jinyong Zhang, Ying Li, Chenghui Zhang, Duo Li, Yiquan Zhou, Hongning Li, Nan Xiao, Pengpeng Front Cell Infect Microbiol Cellular and Infection Microbiology We collected 5,500 mosquitoes belonging to six species in three locations in China. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The affluent viral sequences that were detected and annotated belong to 22 viral taxonomic families. Then, PCR was performed to confirm the results, followed by phylogenetic analysis. Herein, part of mosquito virome was identified, including chikungunya virus (CHIKV), Getah virus (GETV), and Ross river virus (RRV). After metagenomic analysis, seven CHIKV sequences were verified by PCR amplification, among which CHIKV-China/YN2018-1 had the highest homology with the CHIKV isolated in Senegal, 1983, with a nucleotide (nt) identity of at least 81%, belonging to genotype West Africa viral genes. Five GETV sequences were identified, which had a high homology with the GETV sequences isolated from Equus caballus in Japan, 1978, with a (nt) identity of at least 97%. The newly isolated virus CHIKV-China/YN2018-1 became more infectious after passage of the BHK-21 cell line to the Vero cell line. The newly identified RRV gene had the highest homology with the 2006 RRV isolate from Australia, with a (nt) identity of at least 94%. In addition, numerous known and unknown viruses have also been detected in mosquitoes from Yunnan province, China, and propagation tests will be carried out. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8878809/ /pubmed/35223559 http://dx.doi.org/10.3389/fcimb.2022.849662 Text en Copyright © 2022 Feng, Zhang, Zhang, Li, Zhang, Li, Zhou, Li and Xiao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Feng, Guanrong Zhang, Jinyong Zhang, Ying Li, Chenghui Zhang, Duo Li, Yiquan Zhou, Hongning Li, Nan Xiao, Pengpeng Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title | Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title_full | Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title_fullStr | Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title_full_unstemmed | Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title_short | Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses |
title_sort | metagenomic analysis of togaviridae in mosquito viromes isolated from yunnan province in china reveals genes from chikungunya and ross river viruses |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878809/ https://www.ncbi.nlm.nih.gov/pubmed/35223559 http://dx.doi.org/10.3389/fcimb.2022.849662 |
work_keys_str_mv | AT fengguanrong metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT zhangjinyong metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT zhangying metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT lichenghui metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT zhangduo metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT liyiquan metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT zhouhongning metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT linan metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses AT xiaopengpeng metagenomicanalysisoftogaviridaeinmosquitoviromesisolatedfromyunnanprovinceinchinarevealsgenesfromchikungunyaandrossriverviruses |