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Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease

The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this pro...

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Autores principales: Summers, Stacie C., Quimby, Jessica, Blake, Amanda, Keys, Deborah, Steiner, Joerg M., Suchodolski, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878831/
https://www.ncbi.nlm.nih.gov/pubmed/35202337
http://dx.doi.org/10.3390/vetsci9020084
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author Summers, Stacie C.
Quimby, Jessica
Blake, Amanda
Keys, Deborah
Steiner, Joerg M.
Suchodolski, Jan
author_facet Summers, Stacie C.
Quimby, Jessica
Blake, Amanda
Keys, Deborah
Steiner, Joerg M.
Suchodolski, Jan
author_sort Summers, Stacie C.
collection PubMed
description The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this prospective cross-sectional study. Sera were analyzed for 25 AA concentrations using an ion exchange chromatography AA analyzer with post column ninhydrin derivatization. Voided fecal samples were analyzed for 22 AA concentrations using liquid chromatography with tandem mass spectrometry. CKD cats had lower serum concentrations of phenylalanine (mean difference ± standard error of the mean: 12.7 ± 4.3 µM; p = 0.03), threonine (29.6 ± 9.2 µM; p = 0.03), tryptophan (18.4 ± 5.4 µM; p = 0.005), serine (29.8 ± 12.6 µM; p = 0.03), and tyrosine (11.6 ± 3.8 µM; p = 0.01) and higher serum concentrations of aspartic acid (4.7 ± 2.0 µM; p = 0.01), β-alanine (3.4 ± 1.2 µM; p = 0.01), citrulline (5.7 ± 1.6 µM; p = 0.01), and taurine (109.9 ± 29.6 µM; p = 0.01) when compared to healthy cats. Fecal AA concentrations did not differ between healthy cats and CKD cats. 3-Methylhistidine-to-creatinine did not differ between healthy cats with and without muscle loss. Cats with CKD IRIS Stages 1–4 have a deranged serum amino acid profile compared to healthy cats.
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spelling pubmed-88788312022-02-26 Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease Summers, Stacie C. Quimby, Jessica Blake, Amanda Keys, Deborah Steiner, Joerg M. Suchodolski, Jan Vet Sci Article The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this prospective cross-sectional study. Sera were analyzed for 25 AA concentrations using an ion exchange chromatography AA analyzer with post column ninhydrin derivatization. Voided fecal samples were analyzed for 22 AA concentrations using liquid chromatography with tandem mass spectrometry. CKD cats had lower serum concentrations of phenylalanine (mean difference ± standard error of the mean: 12.7 ± 4.3 µM; p = 0.03), threonine (29.6 ± 9.2 µM; p = 0.03), tryptophan (18.4 ± 5.4 µM; p = 0.005), serine (29.8 ± 12.6 µM; p = 0.03), and tyrosine (11.6 ± 3.8 µM; p = 0.01) and higher serum concentrations of aspartic acid (4.7 ± 2.0 µM; p = 0.01), β-alanine (3.4 ± 1.2 µM; p = 0.01), citrulline (5.7 ± 1.6 µM; p = 0.01), and taurine (109.9 ± 29.6 µM; p = 0.01) when compared to healthy cats. Fecal AA concentrations did not differ between healthy cats and CKD cats. 3-Methylhistidine-to-creatinine did not differ between healthy cats with and without muscle loss. Cats with CKD IRIS Stages 1–4 have a deranged serum amino acid profile compared to healthy cats. MDPI 2022-02-17 /pmc/articles/PMC8878831/ /pubmed/35202337 http://dx.doi.org/10.3390/vetsci9020084 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Summers, Stacie C.
Quimby, Jessica
Blake, Amanda
Keys, Deborah
Steiner, Joerg M.
Suchodolski, Jan
Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title_full Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title_fullStr Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title_full_unstemmed Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title_short Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
title_sort serum and fecal amino acid profiles in cats with chronic kidney disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878831/
https://www.ncbi.nlm.nih.gov/pubmed/35202337
http://dx.doi.org/10.3390/vetsci9020084
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