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Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease
The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this pro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878831/ https://www.ncbi.nlm.nih.gov/pubmed/35202337 http://dx.doi.org/10.3390/vetsci9020084 |
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author | Summers, Stacie C. Quimby, Jessica Blake, Amanda Keys, Deborah Steiner, Joerg M. Suchodolski, Jan |
author_facet | Summers, Stacie C. Quimby, Jessica Blake, Amanda Keys, Deborah Steiner, Joerg M. Suchodolski, Jan |
author_sort | Summers, Stacie C. |
collection | PubMed |
description | The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this prospective cross-sectional study. Sera were analyzed for 25 AA concentrations using an ion exchange chromatography AA analyzer with post column ninhydrin derivatization. Voided fecal samples were analyzed for 22 AA concentrations using liquid chromatography with tandem mass spectrometry. CKD cats had lower serum concentrations of phenylalanine (mean difference ± standard error of the mean: 12.7 ± 4.3 µM; p = 0.03), threonine (29.6 ± 9.2 µM; p = 0.03), tryptophan (18.4 ± 5.4 µM; p = 0.005), serine (29.8 ± 12.6 µM; p = 0.03), and tyrosine (11.6 ± 3.8 µM; p = 0.01) and higher serum concentrations of aspartic acid (4.7 ± 2.0 µM; p = 0.01), β-alanine (3.4 ± 1.2 µM; p = 0.01), citrulline (5.7 ± 1.6 µM; p = 0.01), and taurine (109.9 ± 29.6 µM; p = 0.01) when compared to healthy cats. Fecal AA concentrations did not differ between healthy cats and CKD cats. 3-Methylhistidine-to-creatinine did not differ between healthy cats with and without muscle loss. Cats with CKD IRIS Stages 1–4 have a deranged serum amino acid profile compared to healthy cats. |
format | Online Article Text |
id | pubmed-8878831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88788312022-02-26 Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease Summers, Stacie C. Quimby, Jessica Blake, Amanda Keys, Deborah Steiner, Joerg M. Suchodolski, Jan Vet Sci Article The purpose of the study was to quantify serum and fecal amino acids (AA) in cats with chronic kidney disease (CKD) and compare to healthy cats. Thirty-five cats with International Renal Interest Society Stage 1–4 CKD and 16 healthy mature adult and senior client-owned cats were included in this prospective cross-sectional study. Sera were analyzed for 25 AA concentrations using an ion exchange chromatography AA analyzer with post column ninhydrin derivatization. Voided fecal samples were analyzed for 22 AA concentrations using liquid chromatography with tandem mass spectrometry. CKD cats had lower serum concentrations of phenylalanine (mean difference ± standard error of the mean: 12.7 ± 4.3 µM; p = 0.03), threonine (29.6 ± 9.2 µM; p = 0.03), tryptophan (18.4 ± 5.4 µM; p = 0.005), serine (29.8 ± 12.6 µM; p = 0.03), and tyrosine (11.6 ± 3.8 µM; p = 0.01) and higher serum concentrations of aspartic acid (4.7 ± 2.0 µM; p = 0.01), β-alanine (3.4 ± 1.2 µM; p = 0.01), citrulline (5.7 ± 1.6 µM; p = 0.01), and taurine (109.9 ± 29.6 µM; p = 0.01) when compared to healthy cats. Fecal AA concentrations did not differ between healthy cats and CKD cats. 3-Methylhistidine-to-creatinine did not differ between healthy cats with and without muscle loss. Cats with CKD IRIS Stages 1–4 have a deranged serum amino acid profile compared to healthy cats. MDPI 2022-02-17 /pmc/articles/PMC8878831/ /pubmed/35202337 http://dx.doi.org/10.3390/vetsci9020084 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Summers, Stacie C. Quimby, Jessica Blake, Amanda Keys, Deborah Steiner, Joerg M. Suchodolski, Jan Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title | Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title_full | Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title_fullStr | Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title_full_unstemmed | Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title_short | Serum and Fecal Amino Acid Profiles in Cats with Chronic Kidney Disease |
title_sort | serum and fecal amino acid profiles in cats with chronic kidney disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878831/ https://www.ncbi.nlm.nih.gov/pubmed/35202337 http://dx.doi.org/10.3390/vetsci9020084 |
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