Cargando…
Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos
Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered as potential therapeutic factors in several diseases, however, only l...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878891/ https://www.ncbi.nlm.nih.gov/pubmed/35202269 http://dx.doi.org/10.3390/toxics10020083 |
_version_ | 1784658768472047616 |
---|---|
author | Chiarelli, Roberto Martino, Chiara Roccheri, Maria Carmela Geraci, Fabiana |
author_facet | Chiarelli, Roberto Martino, Chiara Roccheri, Maria Carmela Geraci, Fabiana |
author_sort | Chiarelli, Roberto |
collection | PubMed |
description | Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered as potential therapeutic factors in several diseases, however, only limited information is available about their impact on aquatic environments. This study used sea urchin embryos (Paracentrotus lividus) to test V toxicity, as it is known they are sensitive to V doses from environmentally relevant to very cytotoxic levels (50 nM; 100 nM; 500 nM; 1 µM; 50 µM; 100 µM; 500 µM; and 1 mM). We used two approaches: The fertilization test (FT) and a protease detection assay after 36 h of exposure. V affected the fertilization percentage and increased morphological abnormalities of both egg and fertilization envelope, in a dose-dependent manner. Moreover, a total of nine gelatinases (with apparent molecular masses ranging from 309 to 22 kDa) were detected, and their proteolytic activity depended on the V concentration. Biochemical characterization shows that some of them could be aspartate proteases, whereas substrate specificity and the Ca(2+)/Zn(2+) requirement suggest that others are similar to mammalian matrix metalloproteinases (MMPs). |
format | Online Article Text |
id | pubmed-8878891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88788912022-02-26 Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos Chiarelli, Roberto Martino, Chiara Roccheri, Maria Carmela Geraci, Fabiana Toxics Article Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered as potential therapeutic factors in several diseases, however, only limited information is available about their impact on aquatic environments. This study used sea urchin embryos (Paracentrotus lividus) to test V toxicity, as it is known they are sensitive to V doses from environmentally relevant to very cytotoxic levels (50 nM; 100 nM; 500 nM; 1 µM; 50 µM; 100 µM; 500 µM; and 1 mM). We used two approaches: The fertilization test (FT) and a protease detection assay after 36 h of exposure. V affected the fertilization percentage and increased morphological abnormalities of both egg and fertilization envelope, in a dose-dependent manner. Moreover, a total of nine gelatinases (with apparent molecular masses ranging from 309 to 22 kDa) were detected, and their proteolytic activity depended on the V concentration. Biochemical characterization shows that some of them could be aspartate proteases, whereas substrate specificity and the Ca(2+)/Zn(2+) requirement suggest that others are similar to mammalian matrix metalloproteinases (MMPs). MDPI 2022-02-10 /pmc/articles/PMC8878891/ /pubmed/35202269 http://dx.doi.org/10.3390/toxics10020083 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chiarelli, Roberto Martino, Chiara Roccheri, Maria Carmela Geraci, Fabiana Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title | Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title_full | Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title_fullStr | Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title_full_unstemmed | Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title_short | Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos |
title_sort | vanadium toxicity monitored by fertilization outcomes and metal related proteolytic activities in paracentrotus lividus embryos |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878891/ https://www.ncbi.nlm.nih.gov/pubmed/35202269 http://dx.doi.org/10.3390/toxics10020083 |
work_keys_str_mv | AT chiarelliroberto vanadiumtoxicitymonitoredbyfertilizationoutcomesandmetalrelatedproteolyticactivitiesinparacentrotuslividusembryos AT martinochiara vanadiumtoxicitymonitoredbyfertilizationoutcomesandmetalrelatedproteolyticactivitiesinparacentrotuslividusembryos AT roccherimariacarmela vanadiumtoxicitymonitoredbyfertilizationoutcomesandmetalrelatedproteolyticactivitiesinparacentrotuslividusembryos AT geracifabiana vanadiumtoxicitymonitoredbyfertilizationoutcomesandmetalrelatedproteolyticactivitiesinparacentrotuslividusembryos |