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Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa
Ovarian tumor domain (OTU)-containing deubiquitinating enzymes (DUBs) are an essential DUB to maintain protein stability in plants and play important roles in plant growth development and stress response. However, there is little genome-wide identification and analysis of the OTU gene family in rice...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878984/ https://www.ncbi.nlm.nih.gov/pubmed/35215984 http://dx.doi.org/10.3390/v14020392 |
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author | Liu, Qiannan Yan, Tingyun Tan, Xiaoxiang Wei, Zhongyan Li, Yanjun Sun, Zongtao Zhang, Hehong Chen, Jianping |
author_facet | Liu, Qiannan Yan, Tingyun Tan, Xiaoxiang Wei, Zhongyan Li, Yanjun Sun, Zongtao Zhang, Hehong Chen, Jianping |
author_sort | Liu, Qiannan |
collection | PubMed |
description | Ovarian tumor domain (OTU)-containing deubiquitinating enzymes (DUBs) are an essential DUB to maintain protein stability in plants and play important roles in plant growth development and stress response. However, there is little genome-wide identification and analysis of the OTU gene family in rice. In this study, we identified 20 genes of the OTU family in rice genome, which were classified into four groups based on the phylogenetic analysis. Their gene structures, conserved motifs and domains, chromosomal distribution, and cis elements in promoters were further studied. In addition, OTU gene expression patterns in response to plant hormone treatments, including SA, MeJA, NAA, BL, and ABA, were investigated by RT-qPCR analysis. The results showed that the expression profile of OsOTU genes exhibited plant hormone-specific expression. Expression levels of most of the rice OTU genes were significantly changed in response to rice stripe virus (RSV), rice black-streaked dwarf virus (RBSDV), Southern rice black-streaked dwarf virus (SRBSDV), and Rice stripe mosaic virus (RSMV). These results suggest that the rice OTU genes are involved in diverse hormone signaling pathways and in varied responses to virus infection, providing new insights for further functional study of OsOTU genes. |
format | Online Article Text |
id | pubmed-8878984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88789842022-02-26 Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa Liu, Qiannan Yan, Tingyun Tan, Xiaoxiang Wei, Zhongyan Li, Yanjun Sun, Zongtao Zhang, Hehong Chen, Jianping Viruses Article Ovarian tumor domain (OTU)-containing deubiquitinating enzymes (DUBs) are an essential DUB to maintain protein stability in plants and play important roles in plant growth development and stress response. However, there is little genome-wide identification and analysis of the OTU gene family in rice. In this study, we identified 20 genes of the OTU family in rice genome, which were classified into four groups based on the phylogenetic analysis. Their gene structures, conserved motifs and domains, chromosomal distribution, and cis elements in promoters were further studied. In addition, OTU gene expression patterns in response to plant hormone treatments, including SA, MeJA, NAA, BL, and ABA, were investigated by RT-qPCR analysis. The results showed that the expression profile of OsOTU genes exhibited plant hormone-specific expression. Expression levels of most of the rice OTU genes were significantly changed in response to rice stripe virus (RSV), rice black-streaked dwarf virus (RBSDV), Southern rice black-streaked dwarf virus (SRBSDV), and Rice stripe mosaic virus (RSMV). These results suggest that the rice OTU genes are involved in diverse hormone signaling pathways and in varied responses to virus infection, providing new insights for further functional study of OsOTU genes. MDPI 2022-02-14 /pmc/articles/PMC8878984/ /pubmed/35215984 http://dx.doi.org/10.3390/v14020392 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Qiannan Yan, Tingyun Tan, Xiaoxiang Wei, Zhongyan Li, Yanjun Sun, Zongtao Zhang, Hehong Chen, Jianping Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title | Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title_full | Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title_fullStr | Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title_full_unstemmed | Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title_short | Genome-Wide Identification and Gene Expression Analysis of the OTU DUB Family in Oryza sativa |
title_sort | genome-wide identification and gene expression analysis of the otu dub family in oryza sativa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878984/ https://www.ncbi.nlm.nih.gov/pubmed/35215984 http://dx.doi.org/10.3390/v14020392 |
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