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Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae

Salmonella Typhimurium, a foodborne pathogen, is a major concern for food safety. Its MDR serovars of animal origin pose a serious threat to the human population. Phage therapy can be an alternative for the treatment of such MDR Salmonella serovars. In this study, we report on detailed genome analys...

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Autores principales: Sattar, Sadia, Ullah, Inam, Khanum, Sofia, Bailie, Marc, Shamsi, Bushra, Ahmed, Ibrar, Abbas Shah, Tahir, Javed, Sundus, Ghafoor, Aamir, Pervaiz, Amna, Sohail, Fakiha, Imdad, Kaleem, Tariq, Aamira, Bostan, Nazish, Ali, Ijaz, Altermann, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879037/
https://www.ncbi.nlm.nih.gov/pubmed/35215834
http://dx.doi.org/10.3390/v14020241
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author Sattar, Sadia
Ullah, Inam
Khanum, Sofia
Bailie, Marc
Shamsi, Bushra
Ahmed, Ibrar
Abbas Shah, Tahir
Javed, Sundus
Ghafoor, Aamir
Pervaiz, Amna
Sohail, Fakiha
Imdad, Kaleem
Tariq, Aamira
Bostan, Nazish
Ali, Ijaz
Altermann, Eric
author_facet Sattar, Sadia
Ullah, Inam
Khanum, Sofia
Bailie, Marc
Shamsi, Bushra
Ahmed, Ibrar
Abbas Shah, Tahir
Javed, Sundus
Ghafoor, Aamir
Pervaiz, Amna
Sohail, Fakiha
Imdad, Kaleem
Tariq, Aamira
Bostan, Nazish
Ali, Ijaz
Altermann, Eric
author_sort Sattar, Sadia
collection PubMed
description Salmonella Typhimurium, a foodborne pathogen, is a major concern for food safety. Its MDR serovars of animal origin pose a serious threat to the human population. Phage therapy can be an alternative for the treatment of such MDR Salmonella serovars. In this study, we report on detailed genome analyses of a novel Salmonella phage (Salmonella-Phage-SSBI34) and evaluate its therapeutic potential. The phage was evaluated for latent time, burst size, host range, and bacterial growth reduction in liquid cultures. The phage stability was examined at various pH levels and temperatures. The genome analysis (141.095 Kb) indicated that its nucleotide sequence is novel, as it exhibited only 1–7% DNA coverage. The phage genome features 44% GC content, and 234 putative open reading frames were predicted. The genome was predicted to encode for 28 structural proteins and 40 enzymes related to nucleotide metabolism, DNA modification, and protein synthesis. Further, the genome features 11 tRNA genes for 10 different amino acids, indicating alternate codon usage, and hosts a unique hydrolase for bacterial lysis. This study provides new insights into the subfamily Vequintavirinae, of which SSBI34 may represent a new genus.
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spelling pubmed-88790372022-02-26 Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae Sattar, Sadia Ullah, Inam Khanum, Sofia Bailie, Marc Shamsi, Bushra Ahmed, Ibrar Abbas Shah, Tahir Javed, Sundus Ghafoor, Aamir Pervaiz, Amna Sohail, Fakiha Imdad, Kaleem Tariq, Aamira Bostan, Nazish Ali, Ijaz Altermann, Eric Viruses Article Salmonella Typhimurium, a foodborne pathogen, is a major concern for food safety. Its MDR serovars of animal origin pose a serious threat to the human population. Phage therapy can be an alternative for the treatment of such MDR Salmonella serovars. In this study, we report on detailed genome analyses of a novel Salmonella phage (Salmonella-Phage-SSBI34) and evaluate its therapeutic potential. The phage was evaluated for latent time, burst size, host range, and bacterial growth reduction in liquid cultures. The phage stability was examined at various pH levels and temperatures. The genome analysis (141.095 Kb) indicated that its nucleotide sequence is novel, as it exhibited only 1–7% DNA coverage. The phage genome features 44% GC content, and 234 putative open reading frames were predicted. The genome was predicted to encode for 28 structural proteins and 40 enzymes related to nucleotide metabolism, DNA modification, and protein synthesis. Further, the genome features 11 tRNA genes for 10 different amino acids, indicating alternate codon usage, and hosts a unique hydrolase for bacterial lysis. This study provides new insights into the subfamily Vequintavirinae, of which SSBI34 may represent a new genus. MDPI 2022-01-25 /pmc/articles/PMC8879037/ /pubmed/35215834 http://dx.doi.org/10.3390/v14020241 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sattar, Sadia
Ullah, Inam
Khanum, Sofia
Bailie, Marc
Shamsi, Bushra
Ahmed, Ibrar
Abbas Shah, Tahir
Javed, Sundus
Ghafoor, Aamir
Pervaiz, Amna
Sohail, Fakiha
Imdad, Kaleem
Tariq, Aamira
Bostan, Nazish
Ali, Ijaz
Altermann, Eric
Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title_full Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title_fullStr Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title_full_unstemmed Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title_short Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae
title_sort genome analysis and therapeutic evaluation of a novel lytic bacteriophage of salmonella typhimurium: suggestive of a new genus in the subfamily vequintavirinae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879037/
https://www.ncbi.nlm.nih.gov/pubmed/35215834
http://dx.doi.org/10.3390/v14020241
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