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Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, w...

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Autores principales: Eaknai, Waleewan, Bunwatcharaphansakun, Phichaporn, Phungbun, Chutikorn, Jantimaporn, Angkana, Chaisri, Sasikan, Boonrungsiman, Suwimon, Nimmannit, Ubonthip, Khongkow, Mattaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879298/
https://www.ncbi.nlm.nih.gov/pubmed/35215366
http://dx.doi.org/10.3390/ph15020254
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author Eaknai, Waleewan
Bunwatcharaphansakun, Phichaporn
Phungbun, Chutikorn
Jantimaporn, Angkana
Chaisri, Sasikan
Boonrungsiman, Suwimon
Nimmannit, Ubonthip
Khongkow, Mattaka
author_facet Eaknai, Waleewan
Bunwatcharaphansakun, Phichaporn
Phungbun, Chutikorn
Jantimaporn, Angkana
Chaisri, Sasikan
Boonrungsiman, Suwimon
Nimmannit, Ubonthip
Khongkow, Mattaka
author_sort Eaknai, Waleewan
collection PubMed
description Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, we are the first to define an in vitro collagenase inhibitory activity of fenugreek extract (IC(50) = 0.57 ± 0.02 mg/mL), which is 2.6 times more potent than vitamin C (IC(50) = 1.46 mg/mL). Nanoencapsulation has been applied to improve the extract stability, and subsequently enhanced its bioactivities. Liponiosome encapsulating fenugreek extract (LNF) was prepared using a high-speed homogenizer, resulting in homogeneous spherical nanoparticles with sizes in the range of 174.7 ± 49.2 nm, 0.26 ± 0.04 in PdI, and 46.6 ± 7.4% of entrapment efficiency. LNF formulation significantly facilitated a sustained release and significantly enhanced skin penetration over the extracts, suggesting a potential use of LNF for transdermal delivery. The formulated LNF was highly stable, not toxic to human fibroblast, and was able to enhance cell viability, collagen production, and inhibit MMP1, MMP9, IL-6, and IL-8 secretions compared to the extract in the co-cultured skin model. Therefore, ethanolic fenugreek extract and its developed LNF display molecular mechanisms against skin aging and could potentially be used as an innovative ingredient for the prevention of skin aging.
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spelling pubmed-88792982022-02-26 Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation Eaknai, Waleewan Bunwatcharaphansakun, Phichaporn Phungbun, Chutikorn Jantimaporn, Angkana Chaisri, Sasikan Boonrungsiman, Suwimon Nimmannit, Ubonthip Khongkow, Mattaka Pharmaceuticals (Basel) Article Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, we are the first to define an in vitro collagenase inhibitory activity of fenugreek extract (IC(50) = 0.57 ± 0.02 mg/mL), which is 2.6 times more potent than vitamin C (IC(50) = 1.46 mg/mL). Nanoencapsulation has been applied to improve the extract stability, and subsequently enhanced its bioactivities. Liponiosome encapsulating fenugreek extract (LNF) was prepared using a high-speed homogenizer, resulting in homogeneous spherical nanoparticles with sizes in the range of 174.7 ± 49.2 nm, 0.26 ± 0.04 in PdI, and 46.6 ± 7.4% of entrapment efficiency. LNF formulation significantly facilitated a sustained release and significantly enhanced skin penetration over the extracts, suggesting a potential use of LNF for transdermal delivery. The formulated LNF was highly stable, not toxic to human fibroblast, and was able to enhance cell viability, collagen production, and inhibit MMP1, MMP9, IL-6, and IL-8 secretions compared to the extract in the co-cultured skin model. Therefore, ethanolic fenugreek extract and its developed LNF display molecular mechanisms against skin aging and could potentially be used as an innovative ingredient for the prevention of skin aging. MDPI 2022-02-20 /pmc/articles/PMC8879298/ /pubmed/35215366 http://dx.doi.org/10.3390/ph15020254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eaknai, Waleewan
Bunwatcharaphansakun, Phichaporn
Phungbun, Chutikorn
Jantimaporn, Angkana
Chaisri, Sasikan
Boonrungsiman, Suwimon
Nimmannit, Ubonthip
Khongkow, Mattaka
Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title_full Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title_fullStr Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title_full_unstemmed Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title_short Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
title_sort ethanolic fenugreek extract: its molecular mechanisms against skin aging and the enhanced functions by nanoencapsulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879298/
https://www.ncbi.nlm.nih.gov/pubmed/35215366
http://dx.doi.org/10.3390/ph15020254
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