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Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation
Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, w...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879298/ https://www.ncbi.nlm.nih.gov/pubmed/35215366 http://dx.doi.org/10.3390/ph15020254 |
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author | Eaknai, Waleewan Bunwatcharaphansakun, Phichaporn Phungbun, Chutikorn Jantimaporn, Angkana Chaisri, Sasikan Boonrungsiman, Suwimon Nimmannit, Ubonthip Khongkow, Mattaka |
author_facet | Eaknai, Waleewan Bunwatcharaphansakun, Phichaporn Phungbun, Chutikorn Jantimaporn, Angkana Chaisri, Sasikan Boonrungsiman, Suwimon Nimmannit, Ubonthip Khongkow, Mattaka |
author_sort | Eaknai, Waleewan |
collection | PubMed |
description | Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, we are the first to define an in vitro collagenase inhibitory activity of fenugreek extract (IC(50) = 0.57 ± 0.02 mg/mL), which is 2.6 times more potent than vitamin C (IC(50) = 1.46 mg/mL). Nanoencapsulation has been applied to improve the extract stability, and subsequently enhanced its bioactivities. Liponiosome encapsulating fenugreek extract (LNF) was prepared using a high-speed homogenizer, resulting in homogeneous spherical nanoparticles with sizes in the range of 174.7 ± 49.2 nm, 0.26 ± 0.04 in PdI, and 46.6 ± 7.4% of entrapment efficiency. LNF formulation significantly facilitated a sustained release and significantly enhanced skin penetration over the extracts, suggesting a potential use of LNF for transdermal delivery. The formulated LNF was highly stable, not toxic to human fibroblast, and was able to enhance cell viability, collagen production, and inhibit MMP1, MMP9, IL-6, and IL-8 secretions compared to the extract in the co-cultured skin model. Therefore, ethanolic fenugreek extract and its developed LNF display molecular mechanisms against skin aging and could potentially be used as an innovative ingredient for the prevention of skin aging. |
format | Online Article Text |
id | pubmed-8879298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88792982022-02-26 Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation Eaknai, Waleewan Bunwatcharaphansakun, Phichaporn Phungbun, Chutikorn Jantimaporn, Angkana Chaisri, Sasikan Boonrungsiman, Suwimon Nimmannit, Ubonthip Khongkow, Mattaka Pharmaceuticals (Basel) Article Fenugreek, or Trigonella foenum-graecum L. (family Leguminosae) seeds, are typically used as food supplements to increase postnatal lactation. Fenugreek extract displays antioxidative and anti-inflammatory properties, but its mechanisms against skin aging have not been exploited. In this research, we are the first to define an in vitro collagenase inhibitory activity of fenugreek extract (IC(50) = 0.57 ± 0.02 mg/mL), which is 2.6 times more potent than vitamin C (IC(50) = 1.46 mg/mL). Nanoencapsulation has been applied to improve the extract stability, and subsequently enhanced its bioactivities. Liponiosome encapsulating fenugreek extract (LNF) was prepared using a high-speed homogenizer, resulting in homogeneous spherical nanoparticles with sizes in the range of 174.7 ± 49.2 nm, 0.26 ± 0.04 in PdI, and 46.6 ± 7.4% of entrapment efficiency. LNF formulation significantly facilitated a sustained release and significantly enhanced skin penetration over the extracts, suggesting a potential use of LNF for transdermal delivery. The formulated LNF was highly stable, not toxic to human fibroblast, and was able to enhance cell viability, collagen production, and inhibit MMP1, MMP9, IL-6, and IL-8 secretions compared to the extract in the co-cultured skin model. Therefore, ethanolic fenugreek extract and its developed LNF display molecular mechanisms against skin aging and could potentially be used as an innovative ingredient for the prevention of skin aging. MDPI 2022-02-20 /pmc/articles/PMC8879298/ /pubmed/35215366 http://dx.doi.org/10.3390/ph15020254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eaknai, Waleewan Bunwatcharaphansakun, Phichaporn Phungbun, Chutikorn Jantimaporn, Angkana Chaisri, Sasikan Boonrungsiman, Suwimon Nimmannit, Ubonthip Khongkow, Mattaka Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title | Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title_full | Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title_fullStr | Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title_full_unstemmed | Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title_short | Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation |
title_sort | ethanolic fenugreek extract: its molecular mechanisms against skin aging and the enhanced functions by nanoencapsulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879298/ https://www.ncbi.nlm.nih.gov/pubmed/35215366 http://dx.doi.org/10.3390/ph15020254 |
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